KEGG   ENZYME: 1.1.1.283Help
Entry
EC 1.1.1.283                Enzyme                                 

Name
methylglyoxal reductase (NADPH);
lactaldehyde dehydrogenase (NADP+);
GRE2 (gene name);
methylglyoxal reductase (NADPH-dependent);
lactaldehyde:NADP+ oxidoreductase
Class
Oxidoreductases;
Acting on the CH-OH group of donors;
With NAD+ or NADP+ as acceptor
BRITE hierarchy
Sysname
(S)-lactaldehyde:NADP+ oxidoreductase
Reaction(IUBMB)
(S)-lactaldehyde + NADP+ = 2-oxopropanal + NADPH + H+ [RN:R02260]
Reaction(KEGG)
Substrate
(S)-lactaldehyde [CPD:C00424];
NADP+ [CPD:C00006]
Product
2-oxopropanal [CPD:C00546];
NADPH [CPD:C00005];
H+ [CPD:C00080]
Comment
The enzyme from the yeast Saccharomyces cerevisiae catalyses the reduction of a keto group in a number of compounds, forming enantiopure products. Among the substrates are methylglyoxal (which is reduced to (S)-lactaldehyde) [1,2], 3-methylbutanal [3], hexane-2,5-dione [4] and 3-chloro-1-phenylpropan-1-one [5]. The enzyme differs from EC 1.1.1.78, methylglyoxal reductase (NADH), which is found in mammals, by its coenzyme requirement, reaction direction, and enantiomeric preference.
History
EC 1.1.1.283 created 2005, modified 2013
Pathway
ec00620  Pyruvate metabolism
ec00640  Propanoate metabolism
Orthology
K17741  NADPH-dependent methylglyoxal reductase
Genes
SCE: YOL151W(GRE2)
LTH: KLTH0E09680g KLTH0F04026g
VPO: Kpol_1057p21 Kpol_416p7
ZRO: ZYRO0A13574g ZYRO0B00330g ZYRO0B16742g ZYRO0C18546g ZYRO0E10362g ZYRO0F05214g ZYRO0F18656g ZYRO0G00242g ZYRO0G15400g ZYRO0G22506g
CGR: CAGL0E05170g
NCS: NCAS_0A03090(NCAS0A03090) NCAS_0A07210(NCAS0A07210) NCAS_0C05270(NCAS0C05270) NCAS_0C05980(NCAS0C05980) NCAS_0D02420(NCAS0D02420) NCAS_0D04850(NCAS0D04850) NCAS_0G03820(NCAS0G03820) NCAS_0H00790(NCAS0H00790)
NDI: NDAI_0D00910(NDAI0D00910) NDAI_0D05090(NDAI0D05090) NDAI_0F01560(NDAI0F01560) NDAI_0I02450(NDAI0I02450)
TPF: TPHA_0A00590(TPHA0A00590) TPHA_0F03690(TPHA0F03690) TPHA_0M01160(TPHA0M01160) TPHA_0N01940(TPHA0N01940) TPHA_0O00640(TPHA0O00640)
TBL: TBLA_0A01590(TBLA0A01590) TBLA_0C00270(TBLA0C00270) TBLA_0C00280(TBLA0C00280) TBLA_0C03420(TBLA0C03420) TBLA_0C03430(TBLA0C03430) TBLA_0D00750(TBLA0D00750)
TDL: TDEL_0C00230(TDEL0C00230) TDEL_0C02800(TDEL0C02800) TDEL_0D00270(TDEL0D00270)
KAF: KAFR_0A08710(KAFR0A08710) KAFR_0A08720(KAFR0A08720) KAFR_0C00150(KAFR0C00150)
PIC: PICST_72153(GRE2)
CAL: CAALFM_C206720WA(GRE2) CAALFM_C502860CA(GRP2) CAALFM_C603240WA(CaO19.5611)
 » show all
Taxonomy
Reference
1  [PMID:3896793]
  Authors
Murata K, Fukuda Y, Simosaka M, Watanabe K, Saikusa T, Kimura A.
  Title
Metabolism of 2-oxoaldehyde in yeasts. Purification and characterization of NADPH-dependent methylglyoxal-reducing enzyme from Saccharomyces cerevisiae.
  Journal
Eur J Biochem 151:631-6 (1985)
DOI:10.1111/j.1432-1033.1985.tb09151.x
  Sequence
[sce:YOL151W]
Reference
2  [PMID:12722185]
  Authors
Chen CN, Porubleva L, Shearer G, Svrakic M, Holden LG, Dover JL, Johnston M, Chitnis PR, Kohl DH.
  Title
Associating protein activities with their genes: rapid identification of a gene encoding a methylglyoxal reductase in the yeast Saccharomyces cerevisiae.
  Journal
Yeast 20:545-54 (2003)
DOI:10.1002/yea.979
  Sequence
[sce:YOL151W]
Reference
3  [PMID:16999827]
  Authors
Hauser M, Horn P, Tournu H, Hauser NC, Hoheisel JD, Brown AJ, Dickinson JR
  Title
A transcriptome analysis of isoamyl alcohol-induced filamentation in yeast reveals a novel role for Gre2p as isovaleraldehyde reductase.
  Journal
FEMS Yeast Res 7:84-92 (2007)
DOI:10.1111/j.1567-1364.2006.00151.x
  Sequence
[sce:YOL151W]
Reference
4  [PMID:20237665]
  Authors
Muller M, Katzberg M, Bertau M, Hummel W
  Title
Highly efficient and stereoselective biosynthesis of (2S,5S)-hexanediol with a dehydrogenase from Saccharomyces cerevisiae.
  Journal
Org Biomol Chem 8:1540-50 (2010)
DOI:10.1039/b920869k
  Sequence
[sce:YOL151W]
Reference
5  [PMID:20111861]
  Authors
Choi YH, Choi HJ, Kim D, Uhm KN, Kim HK
  Title
Asymmetric synthesis of (S)-3-chloro-1-phenyl-1-propanol using Saccharomyces cerevisiae reductase with high enantioselectivity.
  Journal
Appl Microbiol Biotechnol 87:185-93 (2010)
DOI:10.1007/s00253-010-2442-5
  Sequence
[sce:YOL151W]
Reference
6  [PMID:20606287]
  Authors
Breicha K, Muller M, Hummel W, Niefind K
  Title
Crystallization and preliminary crystallographic analysis of Gre2p, an NADP(+)-dependent alcohol dehydrogenase from Saccharomyces cerevisiae.
  Journal
Acta Crystallogr Sect F Struct Biol Cryst Commun 66:838-41 (2010)
DOI:10.1107/S1744309110018889
  Sequence
[sce:YOL151W]
Other DBs
ExplorEnz - The Enzyme Database: 1.1.1.283
IUBMB Enzyme Nomenclature: 1.1.1.283
ExPASy - ENZYME nomenclature database: 1.1.1.283
BRENDA, the Enzyme Database: 1.1.1.283
CAS: 78310-66-4

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