KEGG   ENZYME: 1.1.1.319Help
Entry
EC 1.1.1.319                Enzyme                                 

Name
isoeugenol synthase;
IGS1;
t-anol/isoeugenol synthase 1
Class
Oxidoreductases;
Acting on the CH-OH group of donors;
With NAD+ or NADP+ as acceptor
BRITE hierarchy
Sysname
eugenol:NADP+ oxidoreductase (coniferyl acetate reducing)
Reaction(IUBMB)
isoeugenol + acetate + NADP+ = coniferyl acetate + NADPH + H+ [RN:R09931]
Reaction(KEGG)
R09931;
(other) R10289
Show
Substrate
isoeugenol [CPD:C10469];
acetate [CPD:C00033];
NADP+ [CPD:C00006]
Product
coniferyl acetate [CPD:C20225];
NADPH [CPD:C00005];
H+ [CPD:C00080]
Comment
The enzyme acts in the opposite direction. In Ocimum basilicum (sweet basil), Clarkia breweri and Petunia hybrida only isoeugenol is formed [1,2]. However in Pimpinella anisum (anise) only anol is formed in vivo, although the cloned enzyme does produce isoeugenol [3].
History
EC 1.1.1.319 created 2012
Pathway
ec00940  Phenylpropanoid biosynthesis
Orthology
K17056  isoeugenol synthase
Reference
1  [PMID:16782809]
  Authors
Koeduka T, Fridman E, Gang DR, Vassao DG, Jackson BL, Kish CM, Orlova I, Spassova SM, Lewis NG, Noel JP, Baiga TJ, Dudareva N, Pichersky E
  Title
Eugenol and isoeugenol, characteristic aromatic constituents of spices, are biosynthesized via reduction of a coniferyl alcohol ester.
  Journal
Proc Natl Acad Sci U S A 103:10128-33 (2006)
DOI:10.1073/pnas.0603732103
  Sequence
Reference
2  [PMID:18208524]
  Authors
Koeduka T, Louie GV, Orlova I, Kish CM, Ibdah M, Wilkerson CG, Bowman ME, Baiga TJ, Noel JP, Dudareva N, Pichersky E
  Title
The multiple phenylpropene synthases in both Clarkia breweri and Petunia hybrida  represent two distinct protein lineages.
  Journal
Plant J 54:362-74 (2008)
DOI:10.1111/j.1365-313X.2008.03412.x
Reference
3  [PMID:18987218]
  Authors
Koeduka T, Baiga TJ, Noel JP, Pichersky E
  Title
Biosynthesis of t-anethole in anise: characterization of t-anol/isoeugenol synthase and an O-methyltransferase specific for a C7-C8 propenyl side chain.
  Journal
Plant Physiol 149:384-94 (2009)
DOI:10.1104/pp.108.128066
Other DBs
ExplorEnz - The Enzyme Database: 1.1.1.319
IUBMB Enzyme Nomenclature: 1.1.1.319
ExPASy - ENZYME nomenclature database: 1.1.1.319
BRENDA, the Enzyme Database: 1.1.1.319

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