enzyme:1.1.1.n12 : No such data.

KEGG   ENZYME: 4.2.1.107Help
Entry
EC 4.2.1.107                Enzyme                                 

Name
3alpha,7alpha,12alpha-trihydroxy-5beta-cholest-24-enoyl-CoA hydratase;
46 kDa hydratase 2;
(24R,25R)-3alpha,7alpha,12alpha,24-tetrahydroxy-5beta-cholestanoyl-CoA hydro-lyase
Class
Lyases;
Carbon-oxygen lyases;
Hydro-lyases
BRITE hierarchy
Sysname
(24R,25R)-3alpha,7alpha,12alpha,24-tetrahydroxy-5beta-cholestanoyl-CoA hydro-lyase [(24E)-3alpha,7alpha,12alpha-trihydroxy-5beta-cholest-24-enoyl-CoA-forming]
Reaction(IUBMB)
(24R,25R)-3alpha,7alpha,12alpha,24-tetrahydroxy-5beta-cholestanoyl-CoA = (24E)-3alpha,7alpha,12alpha-trihydroxy-5beta-cholest-24-enoyl-CoA + H2O [RN:R04813]
Reaction(KEGG)
R04813;
(other) R04809
Show
Substrate
(24R,25R)-3alpha,7alpha,12alpha,24-tetrahydroxy-5beta-cholestanoyl-CoA [CPD:C15614]
Product
(24E)-3alpha,7alpha,12alpha-trihydroxy-5beta-cholest-24-enoyl-CoA [CPD:C05460];
H2O [CPD:C00001]
Comment
This enzyme forms part of the rat peroxisomal multifunctional enzyme perMFE-2, which also exhibits a dehydrogenase activity. The enzyme is involved in the beta-oxidation of the cholesterol side chain in the cholic-acid-biosynthesis pathway.
History
EC 4.2.1.107 created 2005
Pathway
ec00120  Primary bile acid biosynthesis
ec01100  Metabolic pathways
Orthology
K12405  (3R)-3-hydroxyacyl-CoA dehydrogenase / 3a,7a,12a-trihydroxy-5b-cholest-24-enoyl-CoA hydratase / enoyl-CoA hydratase 2
Genes
HSA: 3295(HSD17B4)
PTR: 462019(HSD17B4)
PPS: 100981028(HSD17B4)
GGO: 101129538(HSD17B4)
PON: 100448539(HSD17B4)
NLE: 100590035(HSD17B4)
MCC: 698369(HSD17B4)
MCF: 102123675(HSD17B4)
CSAB: 103244395(HSD17B4)
RRO: 104654581(HSD17B4)
RBB: 108517761 108529825(HSD17B4)
CJC: 100390100(HSD17B4)
SBQ: 101036776(HSD17B4)
MMU: 15488(Hsd17b4)
RNO: 79244(Hsd17b4)
CGE: 103161104(Hsd17b4) 107980163
NGI: 103745113(Hsd17b4)
HGL: 101713520(Hsd17b4)
OCU: 100346781(HSD17B4)
TUP: 102468828(HSD17B4)
CFA: 474630(HSD17B4)
AML: 100471749(HSD17B4)
UMR: 103670554(HSD17B4)
ORO: 101385731(HSD17B4)
FCA: 101091840(HSD17B4)
PTG: 102968873(HSD17B4)
AJU: 106975089(HSD17B4)
BTA: 493643(HSD17B4)
BOM: 102268517(HSD17B4)
PHD: 102329357(HSD17B4)
CHX: 102172142(HSD17B4)
OAS: 101114519(HSD17B4)
SSC: 397574(HSD17B4)
CFR: 102508317(HSD17B4)
CDK: 105104293(HSD17B4)
LVE: 103084787(HSD17B4)
OOR: 101282963(HSD17B4)
ECB: 100034046(HSD17B4)
EPZ: 103548315(HSD17B4)
EAI: 106833039(HSD17B4)
MYB: 102240475(HSD17B4)
MYD: 102767159(HSD17B4)
HAI: 109374101(HSD17B4)
RSS: 109455167(HSD17B4)
PALE: 102887000(HSD17B4)
LAV: 100659299(HSD17B4)
TMU: 101356123
MDO: 100028098(HSD17B4)
SHR: 100932771(HSD17B4)
OAA: 100081754(HSD17B4)
GGA: 395785(HSD17B4)
MGP: 100545396(HSD17B4)
CJO: 107306670(HSD17B4)
APLA: 101791118(HSD17B4)
ACYG: 106048362(HSD17B4)
TGU: 100218226(HSD17B4)
GFR: 102036502(HSD17B4)
FAB: 101807233(HSD17B4)
PHI: 102109547(HSD17B4)
PMAJ: 107216347(HSD17B4)
CCAE: 111941198(HSD17B4)
CCW: 104690497(HSD17B4)
FPG: 101917400(HSD17B4)
FCH: 102058406(HSD17B4)
CLV: 102095771(HSD17B4)
EGZ: 104122491(HSD17B4)
ASN: 102371766(HSD17B4)
AMJ: 102571005(HSD17B4)
PSS: 102451304(HSD17B4)
CMY: 102933522(HSD17B4)
CPIC: 101945395(HSD17B4)
ACS: 100558484(hsd17b4)
PVT: 110077461(SCP2D1) 110084344(HSD17B4)
PBI: 103059748(SCP2D1) 103063870(HSD17B4)
GJA: 107106522(SCP2D1) 107116225(HSD17B4)
XLA: 444492(hsd17b4.L)
XTR: 613082(hsd17b4)
NPR: 108787696(HSD17B4)
DRE: 393105(hsd17b4)
SRX: 107713552(hsd17b4) 107713572
SANH: 107703629(hsd17b4)
SGH: 107571774(hsd17b4)
IPU: 108265438(hsd17b4)
AMEX: 103046836(hsd17b4)
TRU: 101062498(hsd17b4)
LCO: 104920820(hsd17b4)
NCC: 104966339(hsd17b4)
MZE: 101482787(hsd17b4)
OLA: 101159921(hsd17b4)
XMA: 102223774(hsd17b4)
PRET: 103470095(hsd17b4)
NFU: 107394149(hsd17b4)
KMR: 108233704(hsd17b4)
CSEM: 103397642(hsd17b4)
LCF: 108882142(hsd17b4)
SDU: 111230866(hsd17b4)
HCQ: 109526033(hsd17b4)
BPEC: 110167024(hsd17b4)
MALB: 109956607(hsd17b4)
SASA: 106585849(hsd17b4)
OTW: 112247069(hsd17b4)
ELS: 105014609(hsd17b4)
SFM: 108938133(hsd17b4)
LCM: 102356548(HSD17B4)
CMK: 103181119(hsd17b4)
CIN: 100187421(hsd17b4)
SPU: 581580(hsd17b4)
APLC: 110987677
DME: Dmel_CG3415(Mfe2)
DSI: Dsimw501_GD29376(Dsim_GD29376)
MDE: 101897498
AAG: 5568633
AME: 409986
BIM: 100745646
BTER: 100648641
SOC: 105192979
AEC: 105149946
ACEP: 105621202
HST: 105188161
DQU: 106744052
CFO: 105248091
LHU: 105679879
PGC: 109854791
PCF: 106793023
NVI: 100123018
MDL: 103579980
TCA: 663655(17-beta-HD4)
DPA: 109536257
NVL: 108562871
BMOR: 101745499
PMAC: 106711197
PRAP: 110991438
HAW: 110370579
PXY: 105391367
API: 100167575
DNX: 107163428
CLEC: 106670504
ZNE: 110835121
TUT: 107364381
CEL: CELE_E04F6.3(maoc-1) CELE_M03A8.1(dhs-28)
CBR: CBG10899(Cbr-dhs-28) CBG13089(Cbr-maoc-1)
CRG: 105326295
MYI: 110453744
OBI: 106871734
EPA: 110252238
ADF: 107337999
AQU: 100640903
DDI: DDB_G0291247(mfeA)
DFA: DFA_02002(mfeA)
TGO: TGME49_234570(HAD2SCP2)
SMIN: v1.2.022875.t1(symbB.v1.2.022875.t1)
CARE: LT85_3600
 » show all
Taxonomy
Reference
1  [PMID:9371691]
  Authors
Qin YM, Haapalainen AM, Conry D, Cuebas DA, Hiltunen JK, Novikov DK.
  Title
Recombinant 2-enoyl-CoA hydratase derived from rat peroxisomal multifunctional enzyme 2: role of the hydratase reaction in bile acid synthesis.
  Journal
Biochem J 328 ( Pt 2):377-82 (1997)
Reference
2  [PMID:8619845]
  Authors
Xu R, Cuebas DA.
  Title
The reactions catalyzed by the inducible bifunctional enzyme of rat liver peroxisomes cannot lead to the formation of bile acids.
  Journal
Biochem Biophys Res Commun 221:271-8 (1996)
DOI:10.1006/bbrc.1996.0585
Reference
3
  Authors
Kinoshita, T., Miyata, M., Ismail, S.M., Fujimoto, Y., Kakinuma, K., Kokawa, N.I. and Morisaki, M.
  Title
Synthesis and determination of stereochemistry of four diastereoisomers at the C-24 and C-25 positions of 3alpha,7alpha,12alpha,24-tetrahydroxy-5beta-cholestan-26-oic acid and cholic acid.
  Journal
Chem Pharm Bull 36:134-141 (1988)
Reference
4
  Authors
Fujimoto, Y., Kinoshita, T., Oya, I., Kakinuma, K., Ismail, S.M., Sonoda, Y., Sato, Y. and Morisaki, M.
  Title
Non-stereoselective conversion of the four diastereoisomers at the C-24 and C-25 positions of 3alpha,7alpha,12alpha,24-tetrahydroxy-5beta-cholestan-26-oic acid and cholic acid.
  Journal
Chem Pharm Bull 36:142-145 (1988)
Reference
5  [PMID:11146090]
  Authors
Kurosawa T, Sato M, Nakano H, Fujiwara M, Murai T, Yoshimura T, Hashimoto T.
  Title
Conjugation reactions catalyzed by bifunctional proteins related to beta-oxidation in bile acid biosynthesis.
  Journal
Steroids 66:107-14 (2001)
DOI:10.1016/S0039-128X(00)00217-8
Reference
6  [PMID:12543708]
  Authors
Russell DW.
  Title
The enzymes, regulation, and genetics of bile acid synthesis.
  Journal
Annu Rev Biochem 72:137-74 (2003)
DOI:10.1146/annurev.biochem.72.121801.161712
Other DBs
ExplorEnz - The Enzyme Database: 4.2.1.107
IUBMB Enzyme Nomenclature: 4.2.1.107
ExPASy - ENZYME nomenclature database: 4.2.1.107
BRENDA, the Enzyme Database: 4.2.1.107
CAS: 152787-68-3

KEGG   ENZYME: 4.2.1.119Help
Entry
EC 4.2.1.119                Enzyme                                 

Name
enoyl-CoA hydratase 2;
2-enoyl-CoA hydratase 2;
AtECH2;
ECH2;
MaoC;
MFE-2;
PhaJAc;
D-3-hydroxyacyl-CoA hydro-lyase;
D-specific 2-trans-enoyl-CoA hydratase
Class
Lyases;
Carbon-oxygen lyases;
Hydro-lyases
BRITE hierarchy
Sysname
(3R)-3-hydroxyacyl-CoA hydro-lyase
Reaction(IUBMB)
(3R)-3-hydroxyacyl-CoA = (2E)-2-enoyl-CoA + H2O [RN:R09698]
Reaction(KEGG)
Substrate
(3R)-3-hydroxyacyl-CoA [CPD:C01086]
Product
(2E)-2-enoyl-CoA [CPD:C00658];
H2O [CPD:C00001]
Comment
This enzyme catalyses a hydration step in peroxisomal beta-oxidation. The human multifunctional enzyme type 2 (MFE-2) is a 79000 Da enzyme composed of three functional units: (3R)-hydroxyacyl-CoA dehydrogenase, 2-enoyl-CoA hydratase 2 and sterol carrier protein 2-like units [1]. The enzymes from Aeromonas caviae [4] and Arabidopsis thaliana [5] are monofunctional enzymes. 2-Enoyl-CoA hydratase 3 from Candida tropicalis is a part from multifunctional enzyme type 2 [3].
History
EC 4.2.1.119 created 2009
Pathway
ec01040  Biosynthesis of unsaturated fatty acids
Orthology
K12405  (3R)-3-hydroxyacyl-CoA dehydrogenase / 3a,7a,12a-trihydroxy-5b-cholest-24-enoyl-CoA hydratase / enoyl-CoA hydratase 2
K19658  peroxisomal enoyl-CoA hydratase 2
K19659  enoyl-CoA hydratase
Genes
HSA: 3295(HSD17B4)
PTR: 462019(HSD17B4)
PPS: 100981028(HSD17B4)
GGO: 101129538(HSD17B4)
PON: 100448539(HSD17B4)
NLE: 100590035(HSD17B4)
MCC: 698369(HSD17B4)
MCF: 102123675(HSD17B4)
CSAB: 103244395(HSD17B4)
RRO: 104654581(HSD17B4)
RBB: 108517761 108529825(HSD17B4)
CJC: 100390100(HSD17B4)
SBQ: 101036776(HSD17B4)
MMU: 15488(Hsd17b4)
RNO: 79244(Hsd17b4)
CGE: 103161104(Hsd17b4) 107980163
NGI: 103745113(Hsd17b4)
HGL: 101713520(Hsd17b4)
OCU: 100346781(HSD17B4)
TUP: 102468828(HSD17B4)
CFA: 474630(HSD17B4)
AML: 100471749(HSD17B4)
UMR: 103670554(HSD17B4)
ORO: 101385731(HSD17B4)
FCA: 101091840(HSD17B4)
PTG: 102968873(HSD17B4)
AJU: 106975089(HSD17B4)
BTA: 493643(HSD17B4)
BOM: 102268517(HSD17B4)
PHD: 102329357(HSD17B4)
CHX: 102172142(HSD17B4)
OAS: 101114519(HSD17B4)
SSC: 397574(HSD17B4)
CFR: 102508317(HSD17B4)
CDK: 105104293(HSD17B4)
LVE: 103084787(HSD17B4)
OOR: 101282963(HSD17B4)
ECB: 100034046(HSD17B4)
EPZ: 103548315(HSD17B4)
EAI: 106833039(HSD17B4)
MYB: 102240475(HSD17B4)
MYD: 102767159(HSD17B4)
HAI: 109374101(HSD17B4)
RSS: 109455167(HSD17B4)
PALE: 102887000(HSD17B4)
LAV: 100659299(HSD17B4)
TMU: 101356123
MDO: 100028098(HSD17B4)
SHR: 100932771(HSD17B4)
OAA: 100081754(HSD17B4)
GGA: 395785(HSD17B4)
MGP: 100545396(HSD17B4)
CJO: 107306670(HSD17B4)
APLA: 101791118(HSD17B4)
ACYG: 106048362(HSD17B4)
TGU: 100218226(HSD17B4)
GFR: 102036502(HSD17B4)
FAB: 101807233(HSD17B4)
PHI: 102109547(HSD17B4)
PMAJ: 107216347(HSD17B4)
CCAE: 111941198(HSD17B4)
CCW: 104690497(HSD17B4)
FPG: 101917400(HSD17B4)
FCH: 102058406(HSD17B4)
CLV: 102095771(HSD17B4)
EGZ: 104122491(HSD17B4)
ASN: 102371766(HSD17B4)
AMJ: 102571005(HSD17B4)
PSS: 102451304(HSD17B4)
CMY: 102933522(HSD17B4)
CPIC: 101945395(HSD17B4)
ACS: 100558484(hsd17b4)
PVT: 110077461(SCP2D1) 110084344(HSD17B4)
PBI: 103059748(SCP2D1) 103063870(HSD17B4)
GJA: 107106522(SCP2D1) 107116225(HSD17B4)
XLA: 444492(hsd17b4.L)
XTR: 613082(hsd17b4)
NPR: 108787696(HSD17B4)
DRE: 393105(hsd17b4)
SRX: 107713552(hsd17b4) 107713572
SANH: 107703629(hsd17b4)
SGH: 107571774(hsd17b4)
IPU: 108265438(hsd17b4)
AMEX: 103046836(hsd17b4)
TRU: 101062498(hsd17b4)
LCO: 104920820(hsd17b4)
NCC: 104966339(hsd17b4)
MZE: 101482787(hsd17b4)
OLA: 101159921(hsd17b4)
XMA: 102223774(hsd17b4)
PRET: 103470095(hsd17b4)
NFU: 107394149(hsd17b4)
KMR: 108233704(hsd17b4)
CSEM: 103397642(hsd17b4)
LCF: 108882142(hsd17b4)
SDU: 111230866(hsd17b4)
HCQ: 109526033(hsd17b4)
BPEC: 110167024(hsd17b4)
MALB: 109956607(hsd17b4)
SASA: 106585849(hsd17b4)
OTW: 112247069(hsd17b4)
ELS: 105014609(hsd17b4)
SFM: 108938133(hsd17b4)
LCM: 102356548(HSD17B4)
CMK: 103181119(hsd17b4)
CIN: 100187421(hsd17b4)
SPU: 581580(hsd17b4)
APLC: 110987677
DME: Dmel_CG3415(Mfe2)
DSI: Dsimw501_GD29376(Dsim_GD29376)
MDE: 101897498
AAG: 5568633
AME: 409986
BIM: 100745646
BTER: 100648641
SOC: 105192979
AEC: 105149946
ACEP: 105621202
HST: 105188161
DQU: 106744052
CFO: 105248091
LHU: 105679879
PGC: 109854791
PCF: 106793023
NVI: 100123018
MDL: 103579980
TCA: 663655(17-beta-HD4)
DPA: 109536257
NVL: 108562871
BMOR: 101745499
PMAC: 106711197
PRAP: 110991438
HAW: 110370579
PXY: 105391367
API: 100167575
DNX: 107163428
CLEC: 106670504
ZNE: 110835121
TUT: 107364381
CEL: CELE_E04F6.3(maoc-1) CELE_M03A8.1(dhs-28)
CBR: CBG10899(Cbr-dhs-28) CBG13089(Cbr-maoc-1)
CRG: 105326295
MYI: 110453744
OBI: 106871734
EPA: 110252238
ADF: 107337999
AQU: 100640903
ATH: AT1G76150(ECH2)
CRB: 17896162
BRP: 103832064
BOE: 106300265
THJ: 104813765
CPAP: 110818108
CIT: 102628415
TCC: 18591652
GRA: 105770003
GHI: 107904979
EGR: 104448895
VRA: 106762546
VAR: 108343556
CCAJ: 109802448
CAM: 101492992
LJA: Lj1g3v2268360.1(Lj1g3v2268360.1) Lj1g3v2268360.2(Lj1g3v2268360.2) Lj1g3v2953590.1(Lj1g3v2953590.1) Lj1g3v2953590.2(Lj1g3v2953590.2)
ADU: 107460260
AIP: 107609751
LANG: 109343989
FVE: 101293547
PPER: 18792035
PMUM: 103321173
PAVI: 110746650
MDM: 103440214
ZJU: 107422728
CSV: 101212798
CMO: 103488374
MCHA: 111014062
CMAX: 111492280
CMOS: 111462566
CPEP: 111806066
RCU: 8267862
JCU: 105635268
HBR: 110636119
POP: 7494669
JRE: 109018894
VVI: 100262653
SLY: 101253171
SPEN: 107005416
SOT: 102584477
CANN: 107850442
INI: 109184502
SIND: 105178076
OEU: 111379149
HAN: 110908076
LSV: 111889535
CCAV: 112513425
BVG: 104884406
SOE: 110790608
NNU: 104603702
OSA: 4347752
DOSA: Os09t0544900-01(Os09g0544900)
BDI: 100844320
ATS: 109758253(LOC109758253)
PDA: 103701068
EGU: 105055161
MUS: 103970614
PEQ: 110023970
AOF: 109848560
ATR: 18424521
PPP: 112281828
DDI: DDB_G0291247(mfeA)
DFA: DFA_02002(mfeA)
TGO: TGME49_234570(HAD2SCP2)
SMIN: v1.2.022875.t1(symbB.v1.2.022875.t1)
VTA: B0498(phaJ)
SPL: Spea_1753
SHL: Shal_1988
FBL: Fbal_0386
AHA: AHA_2422
ASA: ASA_2281(phaJ)
AMED: B224_2076
ACAV: VI35_11530
CARE: LT85_3600
 » show all
Taxonomy
Reference
1  [PMID:15644212]
  Authors
Koski KM, Haapalainen AM, Hiltunen JK, Glumoff T
  Title
Crystal structure of 2-enoyl-CoA hydratase 2 from human peroxisomal multifunctional enzyme type 2.
  Journal
J Mol Biol 345:1157-69 (2005)
DOI:10.1016/j.jmb.2004.11.009
  Sequence
[hsa:3295]
Reference
2  [PMID:9457873]
  Authors
Fukui T, Shiomi N, Doi Y
  Title
Expression and characterization of (R)-specific enoyl coenzyme A hydratase involved in polyhydroxyalkanoate biosynthesis by Aeromonas caviae.
  Journal
J Bacteriol 180:667-73 (1998)
  Sequence
Reference
3  [PMID:12832794]
  Authors
Koski MK, Haapalainen AM, Hiltunen JK, Glumoff T
  Title
Crystallization and preliminary crystallographic data of 2-enoyl-CoA hydratase 2  domain of Candida tropicalis peroxisomal multifunctional enzyme type 2.
  Journal
Acta Crystallogr D Biol Crystallogr 59:1302-5 (2003)
Reference
4  [PMID:11134939]
  Authors
Hisano T, Fukui T, Iwata T, Doi Y
  Title
Crystallization and preliminary X-ray analysis of (R)-specific enoyl-CoA hydratase from Aeromonas caviae involved in polyhydroxyalkanoate biosynthesis.
  Journal
Acta Crystallogr D Biol Crystallogr 57:145-7 (2001)
Reference
5  [PMID:16982622]
  Authors
Goepfert S, Hiltunen JK, Poirier Y
  Title
Identification and functional characterization of a monofunctional peroxisomal enoyl-CoA hydratase 2 that participates in the degradation of even cis-unsaturated fatty acids in Arabidopsis thaliana.
  Journal
J Biol Chem 281:35894-903 (2006)
DOI:10.1074/jbc.M606383200
  Sequence
[ath:AT1G76150]
Reference
6  [PMID:1879422]
  Authors
Engeland K, Kindl H
  Title
Evidence for a peroxisomal fatty acid beta-oxidation involving D-3-hydroxyacyl-CoAs. Characterization of two forms of hydro-lyase that convert D-(-)-3-hydroxyacyl-CoA into 2-trans-enoyl-CoA.
  Journal
Eur J Biochem 200:171-8 (1991)
DOI:10.1111/j.1432-1033.1991.tb21064.x
Other DBs
ExplorEnz - The Enzyme Database: 4.2.1.119
IUBMB Enzyme Nomenclature: 4.2.1.119
ExPASy - ENZYME nomenclature database: 4.2.1.119
BRENDA, the Enzyme Database: 4.2.1.119

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