KEGG   ENZYME: 1.1.99.36Help
Entry
EC 1.1.99.36                Enzyme                                 

Name
alcohol dehydrogenase (nicotinoprotein);
NDMA-dependent alcohol dehydrogenase;
nicotinoprotein alcohol dehydrogenase;
np-ADH;
ethanol:N,N-dimethyl-4-nitrosoaniline oxidoreductase
Class
Oxidoreductases;
Acting on the CH-OH group of donors;
With unknown physiological acceptors
BRITE hierarchy
Sysname
ethanol:acceptor oxidoreductase
Reaction(IUBMB)
ethanol + acceptor = acetaldehyde + reduced acceptor [RN:R09552]
Reaction(KEGG)
Substrate
ethanol [CPD:C00469];
acceptor [CPD:C00028]
Product
acetaldehyde [CPD:C00084];
reduced acceptor [CPD:C00030]
Comment
Contains Zn2+. Nicotinoprotein alcohol dehydrogenases are unique medium-chain dehydrogenases/reductases (MDR) alcohol dehydrogenases that have a tightly bound NAD+/NADH cofactor that does not dissociate during the catalytic process. Instead, the cofactor is regenerated by a second substrate or electron carrier. While the in vivo electron acceptor is not known, N,N-dimethyl-4-nitrosoaniline (NDMA), which is reduced to 4-(hydroxylamino)-N,N-dimethylaniline, can serve this function in vitro.
The enzyme from the Gram-positive bacterium Amycolatopsis methanolica can accept many primary alcohols as substrates, including benzylalcohol [1].
History
EC 1.1.99.36 created 2010
Orthology
K00119  alcohol dehydrogenase (nicotinoprotein)
Genes
RPX: Rpdx1_4112
MTU: Rv3086(adhD)
MTV: RVBD_3086
MTC: MT3171
MRA: MRA_3118(adhD)
MTF: TBFG_13103
MTB: TBMG_00881
MTK: TBSG_00887
MTZ: TBXG_000873
MTG: MRGA327_18975
MTUR: CFBS_3254(adhD)
MTD: UDA_3086(adhD)
MTN: ERDMAN_3377(adhD)
MTUC: J113_21505
MTUE: J114_16510
MTUH: I917_21670
MTUL: TBHG_03016
MTUT: HKBT1_3241(adhD)
MTUU: HKBT2_3246(adhD)
MTQ: HKBS1_3252(adhD)
MBO: BQ2027_MB3113(adhD)
MBB: BCG_3111(adhD)
MBT: JTY_3106(adhD)
MBM: BCGMEX_3108(adhD)
MBX: BCGT_2936
MAF: MAF_30930(adhD)
MMIC: RN08_3398
MCE: MCAN_31121(adhD)
MCQ: BN44_60602(adhD)
MCV: BN43_60078(adhD)
MCX: BN42_41103(adhD)
MCZ: BN45_60074(adhD)
MPA: MAP_3502(adhD)
MAO: MAP4_0274
MAVI: RC58_01295
MAVU: RE97_01305
MAV: MAV_5214
MLP: MLM_0258
MMI: MMAR_0127(adhD_1) MMAR_3150
MMAE: MMARE11_01140(adhD_1) MMARE11_02250(adhD) MMARE11_38040(adhB_1)
MLI: MULP_00112(adhD_1)
MSG: MSMEI_3308(adhD) MSMEI_4069(adhD)
MVQ: MYVA_5185
MTHN: 4412656_00417(flhA_2) 4412656_01307(frmA)
MAB: MAB_2084
MABB: MASS_4764
MCHE: BB28_22745
MSTE: MSTE_04614(adhB)
MJD: JDM601_2351(adhD_1)
MTER: 4434518_02283(adhD_1) 4434518_02706(adhD) 4434518_03690(flhA_1) 4434518_03868(flhA_2)
NFA: NFA_11970
NFR: ERS450000_02912(flhA_2)
NNO: NONO_c26650(adhB1) NONO_c26770(adhB2) NONO_c26870 NONO_c27570(adhB3)
RRT: 4535765_04510(flhA)
GBR: Gbro_3823
GRU: GCWB2_12080(adhD2) GCWB2_19310(adhD3)
GOM: D7316_02154(adhB_3) D7316_02611(adhB_4) D7316_02982(adhB_5) D7316_03763(adhB_6) D7316_04892(adhD)
TCU: Tcur_4959
FAL: FRAAL0199
GOB: Gobs_4948
BSD: BLASA_0307(adhB) BLASA_4819(adhB2)
MMAR: MODMU_0391(adhB) MODMU_2922(adhB) MODMU_2924(adhB) MODMU_2938(adh) MODMU_5408(adh)
ALO: CRK56109
RXY: Rxyl_3153
STI: Sthe_2859
 » show all
Taxonomy
Reference
1  [PMID:8385013]
  Authors
Van Ophem PW, Van Beeumen J, Duine JA
  Title
Nicotinoprotein [NAD(P)-containing] alcohol/aldehyde oxidoreductases. Purification and characterization of a novel type from Amycolatopsis methanolica.
  Journal
Eur J Biochem 212:819-26 (1993)
DOI:10.1111/j.1432-1033.1993.tb17723.x
  Sequence
Reference
2  [PMID:9485460]
  Authors
Piersma SR, Visser AJ, de Vries S, Duine JA
  Title
Optical spectroscopy of nicotinoprotein alcohol dehydrogenase from Amycolatopsis  methanolica: a comparison with horse liver alcohol dehydrogenase and UDP-galactose epimerase.
  Journal
Biochemistry 37:3068-77 (1998)
DOI:10.1021/bi972115u
Reference
3  [PMID:10784035]
  Authors
Schenkels P, Duine JA
  Title
Nicotinoprotein (NADH-containing) alcohol dehydrogenase from Rhodococcus erythropolis DSM 1069: an efficient catalyst for coenzyme-independent oxidation of a broad spectrum of alcohols and the interconversion of alcohols and aldehydes.
  Journal
Microbiology 146 ( Pt 4):775-85 (2000)
DOI:10.1099/00221287-146-4-775
Reference
4  [PMID:14690248]
  Authors
Piersma SR, Norin A, de Vries S, Jornvall H, Duine JA
  Title
Inhibition of nicotinoprotein (NAD+-containing) alcohol dehydrogenase by trans-4-(N,N-dimethylamino)-cinnamaldehyde binding to the active site.
  Journal
J Protein Chem 22:457-61 (2003)
Reference
5  [PMID:12827287]
  Authors
Norin A, Piersma SR, Duine JA, Jornvall H
  Title
Nicotinoprotein (NAD+ -containing) alcohol dehydrogenase: structural relationships and functional interpretations.
  Journal
Cell Mol Life Sci 60:999-1006 (2003)
DOI:10.1007/s00018-003-3105-9
Other DBs
ExplorEnz - The Enzyme Database: 1.1.99.36
IUBMB Enzyme Nomenclature: 1.1.99.36
ExPASy - ENZYME nomenclature database: 1.1.99.36
BRENDA, the Enzyme Database: 1.1.99.36

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