KEGG   ENZYME: 1.1.99.38Help
Entry
EC 1.1.99.38                Enzyme                                 

Name
2-deoxy-scyllo-inosamine dehydrogenase (AdoMet-dependent);
btrN (gene name);
2-deoxy-scyllo-inosamine dehydrogenase (SAM-dependent)
Class
Oxidoreductases;
Acting on the CH-OH group of donors;
With unknown physiological acceptors
BRITE hierarchy
Sysname
2-deoxy-scyllo-inosamine:S-adenosyl-L-methionine 1-oxidoreductase
Reaction(IUBMB)
2-deoxy-scyllo-inosamine + S-adenosyl-L-methionine = 3-amino-2,3-dideoxy-scyllo-inosose + 5'-deoxyadenosine + L-methionine [RN:R08893]
Reaction(KEGG)
Substrate
2-deoxy-scyllo-inosamine [CPD:C17580];
S-adenosyl-L-methionine [CPD:C00019]
Product
3-amino-2,3-dideoxy-scyllo-inosose [CPD:C17581];
5'-deoxyadenosine [CPD:C05198];
L-methionine [CPD:C00073]
Comment
Involved in the biosynthetic pathway of the aminoglycoside antibiotics of the butirosin family. The enzyme from Bacillus circulans was shown to be a radical S-adenosyl-L-methionine (SAM) enzyme. cf. EC 1.1.1.329, 2-deoxy-scyllo-inosamine dehydrogenase.
History
EC 1.1.99.38 created 2012, modified 2013
Pathway
ec00524  Neomycin, kanamycin and gentamicin biosynthesis
ec01130  Biosynthesis of antibiotics
Orthology
K13549  2-deoxy-scyllo-inosamine dehydrogenase (SAM-dependent)
Reference
1  [PMID:18001019]
  Authors
Yokoyama K, Numakura M, Kudo F, Ohmori D, Eguchi T.
  Title
Characterization and mechanistic study of a radical SAM dehydrogenase in the biosynthesis of butirosin.
  Journal
J Am Chem Soc 129:15147-55 (2007)
DOI:10.1021/ja072481t
  Sequence
Reference
2  [PMID:18672902]
  Authors
Yokoyama K, Ohmori D, Kudo F, Eguchi T.
  Title
Mechanistic study on the reaction of a radical SAM dehydrogenase BtrN by electron paramagnetic resonance spectroscopy.
  Journal
Biochemistry 47:8950-60 (2008)
DOI:10.1021/bi800509x
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 1.1.99.38
IUBMB Enzyme Nomenclature: 1.1.99.38
ExPASy - ENZYME nomenclature database: 1.1.99.38
BRENDA, the Enzyme Database: 1.1.99.38

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