KEGG   ENZYME: 1.12.1.3Help
Entry
EC 1.12.1.3                 Enzyme                                 

Name
hydrogen dehydrogenase (NADP+);
NADP+-linked hydrogenase;
NADP+-reducing hydrogenase;
hydrogenase (ambiguous);
hydrogenase I (ambiguous)
Class
Oxidoreductases;
Acting on hydrogen as donor;
With NAD+ or NADP+ as acceptor
BRITE hierarchy
Sysname
hydrogen:NADP+ oxidoreductase
Reaction(IUBMB)
H2 + NADP+ = H+ + NADPH [RN:R07181]
Reaction(KEGG)
Substrate
H2 [CPD:C00282];
NADP+ [CPD:C00006]
Product
H+ [CPD:C00080];
NADPH [CPD:C00005]
Comment
The protein from the bacterium Desulfovibrio fructosovorans is an iron-sulfur protein that exclusively functions as a hydrogen dehydrogenase [1], while the enzyme from the archaeon Pyrococcus furiosus is a nickel, iron, iron-sulfur protein, that is part of a heterotetrameric complex where the alpha and delta subunits function as a hydrogenase while the beta and gamma subunits function as sulfur reductase (EC 1.12.98.4, sulfhydrogenase). Different from EC 1.12.1.5, hydrogen dehydrogenase [NAD(P)+].
History
EC 1.12.1.3 created 2002, modified 2013
Orthology
K17992  NADP-reducing hydrogenase subunit HndB
K17993  sulfhydrogenase subunit alpha
K17994  sulfhydrogenase subunit delta
K18330  NADP-reducing hydrogenase subunit HndA
K18331  NADP-reducing hydrogenase subunit HndC
K18332  NADP-reducing hydrogenase subunit HndD
Genes
GEB: GM18_1856 GM18_1857 GM18_1858 GM18_1859
PCA: Pcar_1602(hndA-3) Pcar_1603(hndB-3) Pcar_1604(hndC-3) Pcar_1605(hndD-3) Pcar_1633(hndD-2) Pcar_1634(hndC-2) Pcar_1635(hndB-2) Pcar_1636(hndA-2) Pcar_1844(hndC-1) Pcar_1845(hndB-1) Pcar_1846(hndA-1) Pcar_2708(hndC-5)
PPD: Ppro_3516 Ppro_3517 Ppro_3518
PACE: A6070_07085 A6070_07090 A6070_07095 A6070_07100 A6070_07285 A6070_07955 A6070_07960 A6070_07965
PEF: A7E78_02215 A7E78_02220 A7E78_02225 A7E78_02230 A7E78_06775
DMA: DMR_02450 DMR_02460 DMR_02470(nuoF) DMR_02480
DML: Dmul_28600(nuoF3)
DAT: HRM2_16520
DTO: TOL2_C28870 TOL2_C28900
DTI: Desti_2752 Desti_2753
CBEI: LF65_03652
HSC: HVS_11610(hndB1) HVS_12575(hndD2) HVS_12580(hndC2) HVS_12585(hndB2)
CSD: Clst_0900(fhbA) Clst_0902(fdx1) Clst_0903(fhbB) Clst_0904(fhbC)
CCEL: CCDG5_0742 CCDG5_0743(hndC) CCDG5_0744(hndD)
HSD: SD1D_2323 SD1D_2324(hndC) SD1D_2325(hndD)
DRM: Dred_1651
PTH: PTH_2010
AWO: Awo_c26970(hydA1) Awo_c26980(hydB) Awo_c26990(hydD)
TTE: TTE0892 TTE0893(NuoF) TTE0894(NuoG)
TKI: TKV_c19580(hydA1)
TACI: TDSAC_1040
MHG: MHY_20970
PUF: UFO1_1386
LPIL: LIP_0871
MBJ: KQ51_00322(hndB_1) KQ51_00323(hndC_1) KQ51_00324 KQ51_00791(hndD_1) KQ51_01818(hndB_2) KQ51_01835(nuoG)
ATM: ANT_17790
KST: KSMBR1_0596(nuoF_1) KSMBR1_1330(nuoF_2)
PBU: L21SP3_00034(hndD_1) L21SP3_00309(hndD_2) L21SP3_00310(hndC_3)
PBP: STSP1_00009(hndD_1) STSP1_00010(hndC_1) STSP1_01250(hndD_2)
PBAS: SMSP2_01580(hndB) SMSP2_01581(hndC_2) SMSP2_01582(hndD_2) SMSP2_01657(hndD_3)
TPED: TPE_0917
BIP: Bint_0727(nuoF)
EPO: Epro_0734(hydA) Epro_0735(hydB) Epro_0736
FVA: FV113G1_03860(nuoF) FV113G1_03870(nuoG)
TLI: Tlie_1619
BVU: BVU_0396
BOA: Bovatus_04980(hndA) Bovatus_04981(hndD) Bovatus_04982(hndC)
PMUC: ING2E5A_0853(hndB) ING2E5A_0854(hndC) ING2E5A_0855(hndD) ING2E5A_0856(hndA)
BLQ: L21SP5_00769(hndB) L21SP5_00770(hndC_2) L21SP5_00771(hndD_1) L21SP5_00772(hndA_2) L21SP5_00830(hndD_2)
CACI: CLOAM1183(hymB) CLOAM1184
TMA: TM0011
TMW: THMA_0007
TMQ: THMB_0007
TMX: THMC_0007
TPT: Tpet_0912
TRQ: TRQ2_0934
TNA: CTN_0687
TNP: Tnap_0642
TLE: Tlet_1521
TME: Tmel_1171
TAF: THA_1558
THER: Y592_06740
FNO: Fnod_0994
DTN: DTL3_0214
KOL: Kole_0181
DTU: Dtur_0918
PHO: PH1292(PH1292) PH1294(PH1294)
PAB: PAB0640(hydD-2) PAB0641(hydA-2) PAB1786 PAB1787
HTI: HTIA_1871
 » show all
Taxonomy
Reference
1  [PMID:9703971]
  Authors
de Luca G, de Philip P, Rousset M, Belaich JP, Dermoun Z.
  Title
The NADP-reducing hydrogenase of Desulfovibrio fructosovorans: evidence for a native complex with hydrogen-dependent methyl-viologen-reducing activity.
  Journal
Biochem Biophys Res Commun 248:591-6 (1998)
DOI:10.1006/bbrc.1998.9022
  Sequence
Reference
2  [PMID:2538471]
  Authors
Bryant FO, Adams MW
  Title
Characterization of hydrogenase from the hyperthermophilic archaebacterium, Pyrococcus furiosus.
  Journal
J Biol Chem 264:5070-9 (1989)
  Sequence
Reference
3  [PMID:8389482]
  Authors
Ma K, Schicho RN, Kelly RM, Adams MW
  Title
Hydrogenase of the hyperthermophile Pyrococcus furiosus is an elemental sulfur reductase or sulfhydrogenase: evidence for a sulfur-reducing hydrogenase ancestor.
  Journal
Proc Natl Acad Sci U S A 90:5341-4 (1993)
DOI:10.1073/pnas.90.11.5341
Reference
4
  Authors
Ma, K., Zhou, Z.H. and Adams, M.W.
  Title
Hydrogen production from pyruvate by enzymes purified from the hyperthermophilic archaeon, Pyrococcus furiosus: A key role for NADPH.
  Journal
FEMS Microbiol Lett 122:245-250 (1994)
Reference
5  [PMID:18156274]
  Authors
van Haaster DJ, Silva PJ, Hagedoorn PL, Jongejan JA, Hagen WR
  Title
Reinvestigation of the steady-state kinetics and physiological function of the soluble NiFe-hydrogenase I of Pyrococcus furiosus.
  Journal
J Bacteriol 190:1584-7 (2008)
DOI:10.1128/JB.01562-07
Other DBs
ExplorEnz - The Enzyme Database: 1.12.1.3
IUBMB Enzyme Nomenclature: 1.12.1.3
ExPASy - ENZYME nomenclature database: 1.12.1.3
BRENDA, the Enzyme Database: 1.12.1.3
CAS: 9027-05-8

DBGET integrated database retrieval system