KEGG   ENZYME: 1.12.1.4Help
Entry
EC 1.12.1.4                 Enzyme                                 

Name
hydrogenase (NAD+, ferredoxin);
bifurcating [FeFe] hydrogenase
Class
Oxidoreductases;
Acting on hydrogen as donor;
With NAD+ or NADP+ as acceptor
BRITE hierarchy
Sysname
hydrogen:NAD+, ferredoxin oxidoreductase
Reaction(IUBMB)
2 H2 + NAD+ + 2 oxidized ferredoxin = 5 H+ + NADH + 2 reduced ferredoxin [RN:R09508]
Reaction(KEGG)
Substrate
H2 [CPD:C00282];
NAD+ [CPD:C00003];
oxidized ferredoxin [CPD:C00139]
Product
H+ [CPD:C00080];
NADH [CPD:C00004];
reduced ferredoxin [CPD:C00138]
Comment
The enzyme from Thermotoga maritima contains a [FeFe] cluster (H-cluster) and iron-sulfur clusters. It works in the direction evolving hydrogen as a means of eliminating excess reducing equivalents.
History
EC 1.12.1.4 created 2011
Orthology
K17997  iron-hydrogenase subunit alpha
K17998  iron-hydrogenase subunit beta
K17999  iron-hydrogenase subunit gamma
Genes
TMA: TM1424 TM1425 TM1426
TMM: Tmari_1430 Tmari_1431 Tmari_1432
TMI: THEMA_07185 THEMA_07190 THEMA_07195
TMW: THMA_1454 THMA_1455 THMA_1456
TMQ: THMB_1454 THMB_1455 THMB_1456
TMX: THMC_1454 THMC_1455 THMC_1456
TPT: Tpet_1367 Tpet_1368 Tpet_1369
TRQ: TRQ2_1317 TRQ2_1318 TRQ2_1319
TNA: CTN_1067 CTN_1068 CTN_1069
TNP: Tnap_1385 Tnap_1386 Tnap_1387
 » show all
Taxonomy
Reference
1  [PMID:10482784]
  Authors
Verhagen MF, O'Rourke T, Adams MW
  Title
The hyperthermophilic bacterium, Thermotoga maritima, contains an unusually complex iron-hydrogenase: amino acid sequence analyses versus biochemical characterization.
  Journal
Biochim Biophys Acta 1412:212-29 (1999)
DOI:10.1016/S0005-2728(99)00062-6
  Sequence
Reference
2  [PMID:19411328]
  Authors
Schut GJ, Adams MW
  Title
The iron-hydrogenase of Thermotoga maritima utilizes ferredoxin and NADH synergistically: a new perspective on anaerobic hydrogen production.
  Journal
J Bacteriol 191:4451-7 (2009)
DOI:10.1128/JB.01582-08
Other DBs
ExplorEnz - The Enzyme Database: 1.12.1.4
IUBMB Enzyme Nomenclature: 1.12.1.4
ExPASy - ENZYME nomenclature database: 1.12.1.4
BRENDA, the Enzyme Database: 1.12.1.4

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