KEGG   ENZYME: 1.14.11.20Help
Entry
EC 1.14.11.20               Enzyme                                 

Name
deacetoxyvindoline 4-hydroxylase;
desacetoxyvindoline 4-hydroxylase;
desacetyoxyvindoline-17-hydroxylase;
D17H;
desacetoxyvindoline,2-oxoglutarate:oxygen oxidoreductase (4beta-hydroxylating)
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With 2-oxoglutarate as one donor, and incorporation of one atom of oxygen into each donor
BRITE hierarchy
Sysname
deacetoxyvindoline,2-oxoglutarate:oxygen oxidoreductase (4beta-hydroxylating)
Reaction(IUBMB)
deacetoxyvindoline + 2-oxoglutarate + O2 = deacetylvindoline + succinate + CO2 [RN:R05857]
Reaction(KEGG)
Substrate
deacetoxyvindoline [CPD:C02673];
2-oxoglutarate [CPD:C00026];
O2 [CPD:C00007]
Product
deacetylvindoline [CPD:C01091];
succinate [CPD:C00042];
CO2 [CPD:C00011]
Comment
Requires Fe2+ and ascorbate. Also acts on 3-hydroxy-16-methoxy-2,3-dihydrotabersonine and to a lesser extent on 16-methoxy-2,3-dihydrotabersonine.
History
EC 1.14.11.20 created 2002, modified 2005
Pathway
ec00901  Indole alkaloid biosynthesis
ec01110  Biosynthesis of secondary metabolites
Orthology
K12697  deacetoxyvindoline 4-hydroxylase
Reference
1  [PMID:16667836]
  Authors
De Carolis E, Chan F, Balsevich J, De Luca V
  Title
Isolation and Characterization of a 2-Oxoglutarate Dependent Dioxygenase Involved in the Second-to-Last Step in Vindoline Biosynthesis.
  Journal
Plant Physiol 94:1323-1329 (1990)
  Sequence
Reference
2  [PMID:8449913]
  Authors
De Carolis E, De Luca V.
  Title
Purification, characterization, and kinetic analysis of a 2-oxoglutarate-dependent dioxygenase involved in vindoline biosynthesis from Catharanthus roseus.
  Journal
J Biol Chem 268:5504-11 (1993)
  Sequence
Reference
3  [PMID:9701591]
  Authors
Vazquez-Flota FA, De Luca V.
  Title
Developmental and light regulation of desacetoxyvindoline 4-hydroxylase in catharanthus roseus (L.) G. Don. . Evidence Of a multilevel regulatory mechanism
  Journal
Plant Physiol 117:1351-61 (1998)
Other DBs
ExplorEnz - The Enzyme Database: 1.14.11.20
IUBMB Enzyme Nomenclature: 1.14.11.20
ExPASy - ENZYME nomenclature database: 1.14.11.20
BRENDA, the Enzyme Database: 1.14.11.20
CAS: 132084-83-4

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