KEGG   ENZYME: 1.14.11.6Help
Entry
EC 1.14.11.6                Enzyme                                 

Name
thymine dioxygenase;
thymine 7-hydroxylase;
5-hydroxy-methyluracil dioxygenase;
5-hydroxymethyluracil oxygenase
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With 2-oxoglutarate as one donor, and incorporation of one atom of oxygen into each donor
BRITE hierarchy
Sysname
thymine,2-oxoglutarate:oxygen oxidoreductase (7-hydroxylating)
Reaction(IUBMB)
thymine + 2-oxoglutarate + O2 = 5-hydroxymethyluracil + succinate + CO2 [RN:R01412]
Reaction(KEGG)
R01412;
(other) R08610 R08611
Show
Substrate
thymine [CPD:C00178];
2-oxoglutarate [CPD:C00026];
O2 [CPD:C00007]
Product
5-hydroxymethyluracil [CPD:C03088];
succinate [CPD:C00042];
CO2 [CPD:C00011]
Comment
Requires Fe2+ and ascorbate. Also acts on 5-hydroxymethyluracil to oxidize its -CH2OH group first to -CHO and then to -COOH.
History
EC 1.14.11.6 created 1972, modified 1976 (EC 1.14.11.5 created 1972, incorporated 1976)
Orthology
K22406  thymine dioxygenase
K22407  thymine dioxygenase / DNA helicase
Genes
TBR: Tb11.01.5220 Tb927.7.4650
TCR: 508859.74 510357.10
LMA: LMJF_09_1480(JBP1) LMJF_14_0040
LIF: LINJ_09_1560(JBP1) LINJ_14_0040
LDO: LDBPK_091560 LDBPK_140040
LMI: LMXM_09_1480 LMXM_14_0040
LBZ: LBRM_09_1540 LBRM_14_0040
Taxonomy
Reference
1  [PMID:11946494]
  Authors
Bankel L, Holme E, Lindstedt G, Lindstedt S.
  Title
Oxygenases involved in thymine and thymidine metabolism in Neurospora crassa.
  Journal
FEBS Lett 21:135-138 (1972)
DOI:10.1016/0014-5793(72)80121-2
Reference
2  [PMID:4274083]
  Authors
Liu CK, Hsu CA, Abbott MT.
  Title
Catalysis of three sequential dioxygenase reactions by thymine 7-hydroxylase.
  Journal
Arch Biochem Biophys 159:180-7 (1973)
DOI:10.1016/0003-9861(73)90443-8
Reference
3  [PMID:6684117]
  Authors
Warn-Cramer BJ, Macrander LA, Abbott MT.
  Title
Markedly different ascorbate dependencies of the sequential alpha-ketoglutarate dioxygenase reactions catalyzed by an essentially homogeneous thymine 7-hydroxylase from Rhodotorula glutinis.
  Journal
J Biol Chem 258:10551-7 (1983)
Other DBs
ExplorEnz - The Enzyme Database: 1.14.11.6
IUBMB Enzyme Nomenclature: 1.14.11.6
ExPASy - ENZYME nomenclature database: 1.14.11.6
BRENDA, the Enzyme Database: 1.14.11.6
CAS: 37256-67-0

DBGET integrated database retrieval system