KEGG   ENZYME: 1.14.11.9Help
Entry
EC 1.14.11.9                Enzyme                                 

Name
flavanone 3-dioxygenase;
naringenin 3-hydroxylase;
flavanone 3-hydroxylase;
flavanone 3beta-hydroxylase;
flavanone synthase I;
(2S)-flavanone 3-hydroxylase;
naringenin,2-oxoglutarate:oxygen oxidoreductase (3-hydroxylating);
F3H;
flavanone,2-oxoglutarate:oxygen oxidoreductase (3-hydroxylating)
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With 2-oxoglutarate as one donor, and incorporation of one atom of oxygen into each donor
BRITE hierarchy
Sysname
(2S)-flavan-4-one,2-oxoglutarate:oxygen oxidoreductase (3-hydroxylating)
Reaction(IUBMB)
a (2S)-flavan-4-one + 2-oxoglutarate + O2 = a (2R,3R)-dihydroflavonol + succinate + CO2 [RN:R07329]
Reaction(KEGG)
Substrate
(2S)-flavan-4-one [CPD:C02099];
2-oxoglutarate [CPD:C00026];
O2 [CPD:C00007]
Product
(2R,3R)-dihydroflavonol [CPD:C21417];
succinate [CPD:C00042];
CO2 [CPD:C00011]
Comment
Requires Fe2+ and ascorbate. This plant enzyme catalyses an early step in the flavonoid biosynthesis pathway, leading to the production of flavanols and anthocyanins. Substrates include (2S)-naringenin, (2S)-eriodictyol, (2S)-dihydrotricetin and (2S)-pinocembrin. Some enzymes are bifuctional and also catalyse EC 1.14.20.6, flavonol synthase.
History
EC 1.14.11.9 created 1983, modified 1989, modified 2004, modified 2016
Pathway
ec00941  Flavonoid biosynthesis
ec01100  Metabolic pathways
ec01110  Biosynthesis of secondary metabolites
Orthology
K00475  naringenin 3-dioxygenase
Genes
ATH: AT3G51240(F3H)
ALY: ARALYDRAFT_485476
CRB: 17885266
CSAT: 104711665 104780958 104791360
EUS: EUTSA_v10010399mg
BRP: 103841041 103863249 103863374
BNA: 106367722 106444347 106449285 106449286 111197703
BOE: 106307555 106338655
THJ: 104821327
CPAP: 110822393
TCC: 18610772
EGR: 104422756
VRA: 106769061
VAR: 108323664
LJA: Lj3g3v1487200.1(Lj3g3v1487200.1) Lj4g3v1037630.1(Lj4g3v1037630.1)
ADU: 107476683
AIP: 107622822
FVE: 101300182
PPER: 18770366
PMUM: 103340206
PAVI: 110758277
MDM: 103400025(F3H) 103436139(MdF3H)
PXB: 103953484
ZJU: 107424368
RCU: 8268714
JCU: 105629005
JRE: 108997708
SLY: 100736482(F3H)
SPEN: 107011910
SOT: 102577463
CANN: 107859880(F3H)
NTA: 107770893(NtF3H) 107806462
NSY: 104217085
NTO: 104098512
SIND: 105166042
HAN: 110915473
LSV: 111897600
OSA: 9270463
DOSA: Os04t0662600-00(Os04g0662600)
OBR: 102719670
BDI: 100823527
ATS: 109770765(LOC109770765)
SBI: 8082549
ZMA: 542712(fht1)
SITA: 101777126
DCT: 110097388
AOF: 109848156
 » show all
Taxonomy
Reference
1
  Authors
Forkmann, G., Heller, W. and Grisebach, H.
  Title
Anthocyanin biosynthesis in flowers of Matthiola incana flavanone 3- and flavonoid 3'-hydroxylases.
  Journal
Z Naturforsch C: Biosci 35:691-695 (1980)
Reference
2  [PMID:8541503]
  Authors
Charrier B, Coronado C, Kondorosi A, Ratet P
  Title
Molecular characterization and expression of alfalfa (Medicago sativa L.) flavanone-3-hydroxylase and dihydroflavonol-4-reductase encoding genes.
  Journal
Plant Mol Biol 29:773-86 (1995)
Reference
3  [PMID:8685272]
  Authors
Pelletier MK, Shirley BW
  Title
Analysis of flavanone 3-hydroxylase in Arabidopsis seedlings. Coordinate regulation with chalcone synthase and chalcone isomerase.
  Journal
Plant Physiol 111:339-45 (1996)
Reference
4  [PMID:15013767]
  Authors
Wellmann F, Matern U, Lukacin R.
  Title
Significance of C-terminal sequence elements for Petunia flavanone 3beta-hydroxylase activity.
  Journal
FEBS Lett 561:149-54 (2004)
DOI:10.1016/S0014-5793(04)00159-0
Reference
5  [PMID:16147863]
  Authors
Jin Z, Grotewold E, Qu W, Fu G, Zhao D
  Title
Cloning and characterization of a flavanone 3-hydroxylase gene from Saussurea medusa.
  Journal
DNA Seq 16:121-9 (2005)
DOI:10.1080/10425170500050742
Reference
6  [PMID:16779664]
  Authors
Shen G, Pang Y, Wu W, Deng Z, Zhao L, Cao Y, Sun X, Tang K
  Title
Cloning and characterization of a flavanone 3-hydroxylase gene from Ginkgo biloba.
  Journal
Biosci Rep 26:19-29 (2006)
DOI:10.1007/s10540-006-9007-y
Other DBs
ExplorEnz - The Enzyme Database: 1.14.11.9
IUBMB Enzyme Nomenclature: 1.14.11.9
ExPASy - ENZYME nomenclature database: 1.14.11.9
BRENDA, the Enzyme Database: 1.14.11.9
CAS: 75991-43-4

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