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Entry
EC 1.14.12.18               Enzyme                                 

Name
biphenyl 2,3-dioxygenase;
biphenyl dioxygenase
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With NADH or NADPH as one donor, and incorporation of two atoms of oxygen into the other donor
BRITE hierarchy
Sysname
biphenyl,NADH:oxygen oxidoreductase (2,3-hydroxylating)
Reaction(IUBMB)
biphenyl + NADH + H+ + O2 = (1S,2R)-3-phenylcyclohexa-3,5-diene-1,2-diol + NAD+ [RN:R05263]
Reaction(KEGG)
Substrate
biphenyl [CPD:C06588];
NADH [CPD:C00004];
H+ [CPD:C00080];
O2 [CPD:C00007]
Product
(1S,2R)-3-phenylcyclohexa-3,5-diene-1,2-diol [CPD:C06589];
NAD+ [CPD:C00003]
Comment
Requires Fe2+. The enzyme from Burkholderia fungorum LB400 (previously Pseudomonas sp.) is part of a multicomponent system composed of an NADH:ferredoxin oxidoreductase (FAD cofactor), a [2Fe-2S] Rieske-type ferredoxin, and a terminal oxygenase that contains a [2Fe-2S] Rieske-type iron-sulfur cluster and a catalytic mononuclear nonheme iron centre. Chlorine-substituted biphenyls can also act as substrates. Similar to the three-component enzyme systems EC 1.14.12.3 (benzene 1,2-dioxygenase) and EC 1.14.12.11 (toluene dioxygenase).
History
EC 1.14.12.18 created 2001
Pathway
ec00621  Dioxin degradation
ec01100  Metabolic pathways
ec01120  Microbial metabolism in diverse environments
Orthology
K08689  biphenyl 2,3-dioxygenase subunit alpha
K15750  biphenyl 2,3-dioxygenase subunit beta
Genes
PSD: DSC_08750 DSC_08755 DSC_09320
BXE: Bxe_C1196(bphA2) Bxe_C1197(bphA1)
BXB: DR64_8608(bphA) DR64_8609(bphE)
PNA: Pnap_4151 Pnap_4152
ACK: C380_15925(bphA1) C380_15930(bphA2)
CTES: O987_08250 O987_08255
MAAD: AZF01_18225 AZF01_18230
CID: P73_0346 P73_0347
CON: TQ29_19170 TQ29_19175
PGV: SL003B_4095(bphAa) SL003B_4096(bphAb)
RHA: RHA1_ro08059(bphAb) RHA1_ro08060(bphAa)
 » show all
Taxonomy
Reference
1  [PMID:7592331]
  Authors
Haddock JD, Gibson DT.
  Title
Purification and characterization of the oxygenase component of biphenyl 2,3-dioxygenase from Pseudomonas sp. strain LB400.
  Journal
J Bacteriol 177:5834-9 (1995)
DOI:10.1128/JB.177.20.5834-5839.1995
  Sequence
Reference
2  [PMID:9451832]
  Authors
Haddock JD, Pelletier DA, Gibson DT.
  Title
Purification and properties of ferredoxinBPH, a component of biphenyl 2,3-dioxygenase of Pseudomonas sp strain LB400.
  Journal
J Ind Microbiol Biotechnol 19:355-9 (1997)
Reference
3  [PMID:9683647]
  Authors
Broadus RM, Haddock JD.
  Title
Purification and characterization of the NADH:ferredoxinBPH oxidoreductase component of biphenyl 2,3-dioxygenase from Pseudomonas sp. strain LB400.
  Journal
Arch Microbiol 170:106-12 (1998)
Other DBs
ExplorEnz - The Enzyme Database: 1.14.12.18
IUBMB Enzyme Nomenclature: 1.14.12.18
ExPASy - ENZYME nomenclature database: 1.14.12.18
UM-BBD (Biocatalysis/Biodegradation Database): 1.14.12.18
BRENDA, the Enzyme Database: 1.14.12.18
CAS: 103289-55-0

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