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Entry
EC 1.14.12.22               Enzyme                                 

Name
carbazole 1,9a-dioxygenase;
CARDO
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With NADH or NADPH as one donor, and incorporation of two atoms of oxygen into the other donor
BRITE hierarchy
Sysname
9H-carbazole,NAD(P)H:oxygen oxidoreductase (2,3-hydroxylating)
Reaction(IUBMB)
9H-carbazole + NAD(P)H + H+ + O2 = 2'-aminobiphenyl-2,3-diol + NAD(P)+ [RN:R05414 R09512]
Reaction(KEGG)
Substrate
9H-carbazole [CPD:C08060];
NADH [CPD:C00004];
NADPH [CPD:C00005];
H+ [CPD:C00080];
O2 [CPD:C00007]
Product
2'-aminobiphenyl-2,3-diol [CPD:C08061];
NAD+ [CPD:C00003];
NADP+ [CPD:C00006]
Comment
This enzyme catalyses the first reaction in the pathway of carbazole degradation. The enzyme attacks at the 1 and 9a positions of carbazole, resulting in the formation of a highly unstable hemiaminal intermediate that undergoes a spontaneous cleavage and rearomatization, resulting in 2'-aminobiphenyl-2,3-diol. In most bacteria the enzyme is a complex composed of a terminal oxygenase, a ferredoxin, and a ferredoxin reductase. The terminal oxygenase component contains a nonheme iron centre and a Rieske [2Fe-2S] iron-sulfur cluster.
History
EC 1.14.12.22 created 2010
Pathway
ec00621  Dioxin degradation
ec01100  Metabolic pathways
ec01120  Microbial metabolism in diverse environments
Orthology
K15751  carbazole 1,9a-dioxygenase
Genes
PSD: DSC_14580
PRE: PCA10_p0280(carAa)
TTU: TERTU_1259
CYQ: Q91_0967
CZA: CYCME_1637
CYY: CPC19_07895
GBI: PG2T_01265
RBU: PG1C_11575
SSAN: NX02_18955
Taxonomy
Reference
1  [PMID:12450807]
  Authors
Nam JW, Nojiri H, Noguchi H, Uchimura H, Yoshida T, Habe H, Yamane H, Omori T
  Title
Purification and characterization of carbazole 1,9a-dioxygenase, a three-component dioxygenase system of Pseudomonas resinovorans strain CA10.
  Journal
Appl Environ Microbiol 68:5882-90 (2002)
DOI:10.1128/AEM.68.12.5882-5890.2002
  Sequence
Reference
2  [PMID:20368802]
  Authors
Gai Z, Wang X, Liu X, Tai C, Tang H, He X, Wu G, Deng Z, Xu P
  Title
The genes coding for the conversion of carbazole to catechol are flanked by IS6100 elements in Sphingomonas sp. strain XLDN2-5.
  Journal
PLoS One 5:e10018 (2010)
DOI:10.1371/journal.pone.0010018
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 1.14.12.22
IUBMB Enzyme Nomenclature: 1.14.12.22
ExPASy - ENZYME nomenclature database: 1.14.12.22
UM-BBD (Biocatalysis/Biodegradation Database): 1.14.12.22
BRENDA, the Enzyme Database: 1.14.12.22

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