KEGG   ENZYME: 1.14.12.3Help
Entry
EC 1.14.12.3                Enzyme                                 

Name
benzene 1,2-dioxygenase;
benzene hydroxylase;
benzene dioxygenase
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With NADH or NADPH as one donor, and incorporation of two atoms of oxygen into the other donor
BRITE hierarchy
Sysname
benzene,NADH:oxygen oxidoreductase (1,2-hydroxylating)
Reaction(IUBMB)
benzene + NADH + H+ + O2 = cis-cyclohexa-3,5-diene-1,2-diol + NAD+ [RN:R03543]
Reaction(KEGG)
Substrate
benzene [CPD:C01407];
NADH [CPD:C00004];
H+ [CPD:C00080];
O2 [CPD:C00007]
Product
cis-cyclohexa-3,5-diene-1,2-diol [CPD:C04091];
NAD+ [CPD:C00003]
Comment
A system, containing a reductase which is an iron-sulfur flavoprotein (FAD), an iron-sulfur oxygenase and ferredoxin. Requires Fe2+.
History
EC 1.14.12.3 created 1972
Pathway
ec00361  Chlorocyclohexane and chlorobenzene degradation
ec00362  Benzoate degradation
ec01120  Microbial metabolism in diverse environments
Orthology
K03268  benzene/toluene/chlorobenzene dioxygenase subunit alpha
K16268  benzene/toluene/chlorobenzene dioxygenase subunit beta
Genes
PPF: Pput_2880 Pput_2881
PPX: T1E_4266(todC1) T1E_4267(todC2)
PMON: X969_10930 X969_10935
PMOT: X970_10585 X970_10590
BPT: Bpet3738 Bpet3739
Taxonomy
Reference
1  [PMID:4298226]
  Authors
Gibson DT, Koch JR, Kallio RE.
  Title
Oxidative degradation of aromatic hydrocarbons by microorganisms. I. Enzymatic formation of catechol from benzene.
  Journal
Biochemistry 7:2653-62 (1968)
Other DBs
ExplorEnz - The Enzyme Database: 1.14.12.3
IUBMB Enzyme Nomenclature: 1.14.12.3
ExPASy - ENZYME nomenclature database: 1.14.12.3
UM-BBD (Biocatalysis/Biodegradation Database): 1.14.12.3
BRENDA, the Enzyme Database: 1.14.12.3
CAS: 9075-66-5

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