KEGG   ENZYME: 1.14.13.171Help
Entry
EC 1.14.13.171              Enzyme                                 

Name
neopentalenolactone D synthase;
ptlE (gene name)
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor
BRITE hierarchy
Sysname
1-deoxy-11-oxopentalenate,NADH:oxygen oxidoreductase (neopentalenolactone-D forming)
Reaction(IUBMB)
1-deoxy-11-oxopentalenate + NADPH + H+ + O2 = neopentalenolactone D + NADP+ + H2O [RN:R10155]
Reaction(KEGG)
Substrate
1-deoxy-11-oxopentalenate [CPD:C20400];
NADPH [CPD:C00005];
H+ [CPD:C00080];
O2 [CPD:C00007]
Product
neopentalenolactone D [CPD:C20402];
NADP+ [CPD:C00006];
H2O [CPD:C00001]
Comment
A FAD-dependent oxygenase. Isolated from the bacterium Streptomyces avermitilis. The ketone undergoes a biological Baeyer-Villiger reaction.
History
EC 1.14.13.171 created 2012
Pathway
ec00999  Biosynthesis of secondary metabolites - unclassified
Orthology
K18091  pentalenolactone D synthase
Genes
SMA: SAVERM_2994(ptlE)
SBH: SBI_09676
SCI: B446_29680
SCW: TU94_27390
Taxonomy
Reference
1  [PMID:21250661]
  Authors
Seo MJ, Zhu D, Endo S, Ikeda H, Cane DE
  Title
Genome mining in Streptomyces. Elucidation of the role of Baeyer-Villiger monooxygenases and non-heme iron-dependent dehydrogenase/oxygenases in the final  steps of the biosynthesis of pentalenolactone and neopentalenolactone.
  Journal
Biochemistry 50:1739-54 (2011)
DOI:10.1021/bi1019786
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 1.14.13.171
IUBMB Enzyme Nomenclature: 1.14.13.171
ExPASy - ENZYME nomenclature database: 1.14.13.171
BRENDA, the Enzyme Database: 1.14.13.171

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