KEGG   ENZYME: 1.14.13.33Help
Entry
EC 1.14.13.33               Enzyme                                 

Name
4-hydroxybenzoate 3-monooxygenase [NAD(P)H];
4-hydroxybenzoate 3-monooxygenase (reduced nicotinamide adenine dinucleotide (phosphate));
4-hydroxybenzoate-3-hydroxylase;
4-hydroxybenzoate 3-hydroxylase
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor
BRITE hierarchy
Sysname
4-hydroxybenzoate,NAD(P)H:oxygen oxidoreductase (3-hydroxylating)
Reaction(IUBMB)
4-hydroxybenzoate + NAD(P)H + H+ + O2 = 3,4-dihydroxybenzoate + NAD(P)+ + H2O [RN:R01296 R01298]
Reaction(KEGG)
Substrate
4-hydroxybenzoate [CPD:C00156];
NADH [CPD:C00004];
NADPH [CPD:C00005];
H+ [CPD:C00080];
O2 [CPD:C00007]
Product
3,4-dihydroxybenzoate [CPD:C00230];
NAD+ [CPD:C00003];
NADP+ [CPD:C00006];
H2O [CPD:C00001]
Comment
A flavoprotein (FAD). The enzyme from Corynebacterium cyclohexanicum is highly specific for 4-hydroxybenzoate, but uses NADH and NADPH at approximately equal rates (cf. EC 1.14.13.2 4-hydroxybenzoate 3-monooxygenase). It is less specific for NADPH than EC 1.14.13.2.
History
EC 1.14.13.33 created 1989, modified 1999
Pathway
ec00362  Benzoate degradation
ec01100  Metabolic pathways
ec01120  Microbial metabolism in diverse environments
Reference
1  [PMID:3971979]
  Authors
Fujii T, Kaneda T.
  Title
Purification and properties of NADH/NADPH-dependent p-hydroxybenzoate hydroxylase from Corynebacterium cyclohexanicum.
  Journal
Eur J Biochem 147:97-104 (1985)
DOI:10.1111/j.1432-1033.1985.tb08724.x
Reference
2  [PMID:8706756]
  Authors
Seibold B, Matthes M, Eppink MH, Lingens F, Van Berkel WJ, Muller R.
  Title
4-Hydroxybenzoate hydroxylase from Pseudomonas sp. CBS3. Purification, characterization, gene cloning, sequence analysis and assignment of structural features determining the coenzyme specificity.
  Journal
Eur J Biochem 239:469-78 (1996)
Other DBs
ExplorEnz - The Enzyme Database: 1.14.13.33
IUBMB Enzyme Nomenclature: 1.14.13.33
ExPASy - ENZYME nomenclature database: 1.14.13.33
BRENDA, the Enzyme Database: 1.14.13.33
CAS: 95471-33-3

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