KEGG   ENZYME: 1.14.13.59Help
Entry
EC 1.14.13.59               Enzyme                                 

Name
L-lysine N6-monooxygenase (NADPH);
lysine N6-hydroxylase;
L-lysine 6-monooxygenase (NADPH) (ambiguous)
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor
BRITE hierarchy
Sysname
L-lysine,NADPH:oxygen oxidoreductase (6-hydroxylating)
Reaction(IUBMB)
L-lysine + NADPH + H+ + O2 = N6-hydroxy-L-lysine + NADP+ + H2O [RN:R00448]
Reaction(KEGG)
Substrate
L-lysine [CPD:C00047];
NADPH [CPD:C00005];
H+ [CPD:C00080];
O2 [CPD:C00007]
Product
N6-hydroxy-L-lysine [CPD:C01028];
NADP+ [CPD:C00006];
H2O [CPD:C00001]
Comment
A flavoprotein (FAD). The enzyme from strain EN 222 of Escherichia coli is highly specific for L-lysine; L-ornithine and L-homolysine are, for example, not substrates.
History
EC 1.14.13.59 created 1999, modified 2001, modified 2012
Pathway
ec00310  Lysine degradation
ec01100  Metabolic pathways
ec01120  Microbial metabolism in diverse environments
Orthology
K03897  lysine N6-hydroxylase
Genes
EOJ: ECO26_1348(iucD)
EOI: ECO111_1296(iucD)
ECOO: ECRM13514_1293(iucD)
ECOH: ECRM13516_1249(iucD)
ESO: O3O_02285
ESM: O3M_22985
ESL: O3K_23070
ECC: c3624(iucD)
ECV: APECO1_O1CoBM169(iucD)
ECM: EcSMS35_3179(iucD)
ECQ: ECED1_4902(iucD)
ECK: EC55989_4642(iucD)
ECT: ECIAI39_3426(iucD)
EOC: CE10_3449(iucD)
EUM: ECUMN_3372(iucD)
ECZ: ECS88_p0010(iucD)
ELN: NRG857_30237(iucD)
EAB: ECABU_c32820(iucD)
ENA: ECNA114_4698(iucD)
ELC: i14_3317(iucD)
ELD: i02_3317(iucD)
ECOJ: P423_16375
ECOS: EC958_3274(iucD)
SFL: SF3718(iucD)
SFX: S4053(iucD)
SFV: SFV_3851(iucD)
SFE: SFxv_4051
SFN: SFy_5297
SFS: SFyv_5371
SFT: NCTC1_04015(iucD)
SSN: SSON_3604(iucD)
SBO: SBO_4340(iucD)
SBC: SbBS512_E4631(iucD)
ENC: ECL_03319(iucD)
ECLX: LI66_14275
ECLZ: LI64_13685
EEC: EcWSU1_02922(iucD)
CTU: Ctu_1p01070(iucD)
KPU: KP1_p315(iucD)
KPR: KPR_4894(iucD)
KPNK: BN49_pII0065(iucD)
CKO: CKO_00970
CLAP: NCTC11466_02784(iucD)
YPE: YPO0993(iucD) YPO1530(alcA)
YPK: y2640(ysuI) y3384(iucD)
YPH: YPC_0799(iucD)
YPM: YP_1419(alcA) YP_3447(iucD)
YPZ: YPZ3_0745(iucD) YPZ3_1395(alcA)
YPD: YPD4_0700(iucD) YPD4_1361(alcA)
YPX: YPD8_0700(iucD) YPD8_1587(alcA)
YPW: CH59_308 CH59_870(iucD)
YPJ: CH55_1008 CH55_3547(iucD)
YPV: BZ15_2022 BZ15_2580(iucD)
YPL: CH46_3596 CH46_4152(iucD)
YPS: YPTB1542(alcA) YPTB3266(iucD)
YPO: BZ17_3344(iucD) BZ17_973
YPQ: DJ40_3171(iucD) DJ40_670
YPU: BZ21_2546(iucD) BZ21_880
YPR: BZ20_2848(iucD) BZ20_422
YPC: BZ23_1159 BZ23_2827(iucD)
YAL: AT01_1814
YIN: CH53_2434(iucD)
SRL: SOD_c07750(iucD1) SOD_c23600(iucD2) SOD_c47380(iucD)
SPLY: Q5A_004125(iucD_1) Q5A_013040(iucD_2)
SMAF: D781_0884
SMAR: SM39_3651 SM39_4454(iucD)
SERF: L085_07285
PEC: W5S_0841
ETA: ETA_30290(dfoA)
EPY: EpC_32420(dfoA)
EPR: EPYR_03490(dfoA)
EAM: EAMY_3239(dfoA)
EAY: EAM_0360
EBI: EbC_25000(iucD)
ERJ: EJP617_07270(dfoA)
EGE: EM595_p0529(iucD)
PAM: PANA_0353(iucD) PANA_0636(alcA)
PAJ: PAJ_3508(iucD) PAJ_3771(alcA)
PVA: Pvag_pPag30340(dfoA)
PLU: plu4629
XBV: XBW1_3604
XNE: XNC1_1306
XNM: XNC2_1273
XDO: XDD1_1244
XPO: XPG1_1073(rhbE)
VAU: VANGNB10_67p040c(angU)
VFI: VF_A0164(iucD)
VSA: VSAL_I0135(iucD) VSAL_II0278(iucD)
PST: PSPTO_0211(iucD)
PFC: PflA506_2780(pmsF)
PEN: PSEEN2502(basC)
PSEC: CCOS191_2629(rhbE)
PALI: A3K91_1223
PSYC: DABAL43B_1549(rhbE)
ABY: ABAYE1094
ABC: ACICU_02580(basC)
ABN: AB57_2812
ABX: ABK1_2699
ABZ: ABZJ_02774(basC)
ABD: ABTW07_2829(basC)
ABH: M3Q_2847
ABAD: ABD1_16330 ABD1_23780(basC)
ACC: BDGL_001871(basC)
SON: SO_3030(pubA)
SBL: Sbal_2708
SWD: Swoo_0586
PSM: PSM_B0023
PSEO: OM33_21425
PEA: PESP_b0029(iucD)
PTU: PTUN_a4218(iucD)
PTD: PTET_b0027(iucD)
SAGA: M5M_06455
LPO: LPO_2065(iucD)
LPF: lpl1946
LHA: LHA_1351(iucD)
FPH: Fphi_0923
FPT: BZ13_1108
FPI: BF30_1152
FPM: LA56_1255
FPX: KU46_405
FPZ: LA55_1975
FPJ: LA02_330
NOC: Noc_1811
NWA: Nwat_1301
CSA: Csal_1054
HEL: HELO_2824
KKO: Kkor_2040
KGE: TQ33_0707
ADH: CK627_03410(basC)
SALN: SALB1_3682
AMAH: DLM_2747
RSO: RSc1817(iucD)
RSN: RSPO_m01615(iucD)
RSE: F504_1572
BCJ: BCAS0043
BCEO: I35_7041
BCED: DM42_6412
BPC: BPTD_2414(alcA)
BPER: BN118_2542(alcA)
BPET: B1917_1405(alcA)
BPEU: Q425_21680(alcA)
BPAR: BN117_3407(alcA)
BBM: BN115_3565(alcA)
BBH: BN112_4522(alcA)
BBX: BBS798_3674(alcA)
BHO: D560_3389
BHM: D558_3365
AXY: AXYL_03070(alcA)
AXX: ERS451415_02967(iucD)
AMIM: MIM_c25820(alcA1) MIM_c25860(alcA2)
AAV: Aave_4219
AAA: Acav_4092
AOA: dqs_2667
BBA: Bd1573
BBAT: Bdt_1566
BBAC: EP01_04085
BMX: BMS_0525(iucD)
HAX: BALOs_0964(iucD)
MLO: mlr5923
SME: SMa2408(rhbE)
SMEL: SM2011_a2408(rhbE)
ARA: Arad_8229(vbsO)
AVI: Avi_5731
RET: RHE_PF00456(vbsO)
REC: RHECIAT_PC0000681(vbsO)
REL: REMIM1_PF00420(iucD)
REP: IE4803_PC00508(iucD)
REI: IE4771_PD00518(iucD)
RLE: pRL120314(vbsO)
OAN: Oant_3254
OAH: DR92_3235(iucD)
AZC: AZC_3247
MET: M446_5059
MSL: Msil_3318
HDI: HDIA_3128(iucD)
TXI: TH3_13065
BAQ: BACAU_0966(iucD)
BAMP: B938_04825
BAMA: RBAU_0968
BAMN: BASU_0945(rhbE)
BAMB: BAPNAU_2795(hxyA)
BAMT: AJ82_05650
BAMY: V529_09450
BMP: NG74_01015(iucD)
BAO: BAMF_1075(RBAM_010080)
BQL: LL3_01069
BQY: MUS_1025(rhbE)
BAMI: KSO_014840
BAMC: U471_09950
BAMF: U722_05090
BCL: ABC1280
BACP: SB24_04855
BACB: OY17_07705
BEO: BEH_12440
BGY: BGLY_1218
OIH: OB0380
BSE: Bsel_3091
ESI: Exig_1071
EAN: Eab7_1044
PMW: B2K_40340
JEO: JMA_14450
CCJ: UL81_01875(alcA)
GOR: KTR9_0498
GRU: GCWB2_02965(iucD)
SCO: SCO2016(SC7H2.30c) SCO2783(SCC105.14)
SCT: SCAT_1143 SCAT_p1224(alcA)
SALS: SLNWT_5882
STRP: F750_1862 F750_2609(desB)
SAMB: SAM23877_2102(rhbE) SAM23877_2818(desB)
SRW: TUE45_02530(iucD_1) TUE45_03398(iucD_2)
SLE: sle_44570(desB) sle_51230(sle_51230)
SRN: A4G23_01835(iucD_1) A4G23_04771(iucD_2)
SALF: SMD44_02199(iucD) SMD44_03081(iucD)
SALJ: SMD11_5041(iucD)
SLX: SLAV_23920(iucD1) SLAV_27510(iucD2) SLAV_39955(iucD3)
KSK: KSE_53820
CMS: CMS1134
CMC: CMN_02058(alcA)
ART: Arth_0286
ARX: ARZXY2_3649(iucD)
AAU: AAur_0267
ACH: Achl_0513
LMOI: VV02_14845
XCE: Xcel_1802
IDO: I598_1668(iucD)
CFL: Cfla_0197
CFI: Celf_3061
BLIN: BLSMQ_3011
KFL: Kfla_3184
PSIM: KR76_01465
NDA: Ndas_1073
FAL: FRAAL6424
AMD: AMED_2693(iucD)
AMN: RAM_13690
AMM: AMES_2665(iucD)
AMZ: B737_2666(iucD)
AMQ: AMETH_4715(iucD)
SESP: BN6_51340(iucD)
ACTI: UA75_12105
ACAD: UA74_12020
AHG: AHOG_11330(iucD)
ALO: CRK55946
AMS: AMIS_67510(sidC)
ASE: ACPL_7000(sidC)
ACTS: ACWT_6869
TBI: Tbis_3460
RXY: Rxyl_0408
AVA: Ava_2835
CPI: Cpin_2840
FLN: FLA_1337
FJO: Fjoh_3173
EAO: BD94_2299
ELB: VO54_00400(iucD)
CHZ: CHSO_0857
TJE: TJEJU_0710(alcA)
TMAR: MARIT_0170(tbsB)
HAL: VNG_6214G(hxyA)
HSL: OE_5098F(iucD)
HMU: Hmuk_3333
HME: HFX_5041(iucD)
HTU: Htur_0217
NAT: NJ7G_0328
 » show all
Taxonomy
Reference
1  [PMID:2518519]
  Authors
Plattner HJ, Pfefferle P, Romaguera A, Waschutza S, Diekmann H.
  Title
Isolation and some properties of lysine N6-hydroxylase from Escherichia coli strain EN222.
  Journal
Biol Met 2:1-5 (1989)
Reference
2  [PMID:8504838]
  Authors
Macheroux P, Plattner HJ, Romaguera A, Diekmann H.
  Title
FAD and substrate analogs as probes for lysine N6-hydroxylase from Escherichia coli EN 222.
  Journal
Eur J Biochem 213:995-1002 (1993)
DOI:10.1111/j.1432-1033.1993.tb17846.x
Reference
3  [PMID:8218389]
  Authors
Thariath AM, Fatum KL, Valvano MA, Viswanatha T.
  Title
Physico-chemical characterization of a recombinant cytoplasmic form of lysine: N6-hydroxylase.
  Journal
Biochim Biophys Acta 1203:27-35 (1993)
DOI:10.1016/0167-4838(93)90032-M
Reference
4  [PMID:2935523]
  Authors
de Lorenzo V, Bindereif A, Paw BH, Neilands JB.
  Title
Aerobactin biosynthesis and transport genes of plasmid ColV-K30 in Escherichia coli K-12.
  Journal
J Bacteriol 165:570-8 (1986)
DOI:10.1128/JB.165.2.570-578.1986
  Sequence
Reference
5
  Authors
Marrone, L., Siemann, S., Beecroft, M. and Viswanatha, T.
  Title
Specificity of lysine:N-6-hydroxylase: A hypothesis for a reactive substrate intermediate in the catalytic mechanism.
  Journal
Bioorg Chem 24:401-406 (1996)
Reference
6  [PMID:2493814]
  Authors
Goh CJ, Szczepan EW, Menhart N, Viswanatha T.
  Title
Studies on lysine:N6-hydroxylation by cell-free systems of Aerobacter aerogenes 62-1.
  Journal
Biochim Biophys Acta 990:240-5 (1989)
DOI:10.1016/S0304-4165(89)80040-6
Other DBs
ExplorEnz - The Enzyme Database: 1.14.13.59
IUBMB Enzyme Nomenclature: 1.14.13.59
ExPASy - ENZYME nomenclature database: 1.14.13.59
BRENDA, the Enzyme Database: 1.14.13.59
CAS: 64295-82-5

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