KEGG   ENZYME: 1.14.15.18Help
Entry
EC 1.14.15.18               Enzyme                                 

Name
calcidiol 1-monooxygenase;
25-hydroxycholecalciferol 1-hydroxylase;
25-hydroxycholecalciferol 1-monooxygenase;
1-hydroxylase-25-hydroxyvitamin D3;
25-hydroxy D3-1alpha-hydroxylase;
25-hydroxycholecalciferol 1alpha-hydroxylase;
25-hydroxyvitamin D3 1alpha-hydroxylase
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With reduced iron-sulfur protein as one donor, and incorporation of one atom of oxygen into the other donor
BRITE hierarchy
Sysname
calcidiol,adrenodoxin:oxygen oxidoreductase (1-hydroxylating)
Reaction(IUBMB)
(1) calcidiol + 2 reduced adrenodoxin + 2 H+ + O2 = calcitriol + 2 oxidized adrenodoxin + H2O [RN:R03610];
(2) secalciferol + 2 reduced adrenodoxin + 2 H+ + O2 = calcitetrol + 2 oxidized adrenodoxin + H2O [RN:R11324]
Reaction(KEGG)
Substrate
calcidiol [CPD:C01561];
reduced adrenodoxin [CPD:C00662];
H+ [CPD:C00080];
O2 [CPD:C00007];
secalciferol [CPD:C07712]
Product
calcitriol [CPD:C01673];
oxidized adrenodoxin [CPD:C00667];
H2O [CPD:C00001];
calcitetrol [CPD:C18231]
Comment
A P-450 (heme-thiolate) enzyme found in mammals.
History
EC 1.14.15.18 created 1976 as EC 1.14.13.13, transferred 2016 to EC 1.14.15.18
Pathway
ec00100  Steroid biosynthesis
ec01100  Metabolic pathways
Orthology
K07438  calcidiol 1-monooxygenase (25-hydroxyvitamin D3 1alpha-hydroxylase)
Genes
HSA: 1594(CYP27B1)
PTR: 452027(CYP27B1)
PPS: 100974264
GGO: 101146696
PON: 100443915
NLE: 100594622
MCC: 715747(CYP27B1)
MCF: 102117707
CSAB: 103238591(CYP27B1)
RRO: 104682342
RBB: 108524407
CJC: 100388588(CYP27B1)
SBQ: 101042462
MMU: 13115(Cyp27b1)
RNO: 114700(Cyp27b1)
CGE: 100760414
NGI: 103742896
HGL: 101700851
CCAN: 109686086
OCU: 100341053
TUP: 102492875
CFA: 481133(CYP27B1)
AML: 100481484
UMR: 103656705
ORO: 101363421
FCA: 100750221(CYP27B1)
PTG: 102971063
AJU: 106975877
BTA: 539630(CYP27B1)
BOM: 102278257
BIU: 109559305
PHD: 102343052
CHX: 102170758
OAS: 101116039
SSC: 397055(CYP27B1)
CFR: 102522811
CDK: 105100328
BACU: 103001013
LVE: 103084276
OOR: 101284262
ECB: 100034100(CYP27B1)
EPZ: 103553643
EAI: 106833020
MYB: 102262846
MYD: 102757775
HAI: 109390756
RSS: 109453000
PALE: 102894468
LAV: 100657886
TMU: 101355020
MDO: 100031497
SHR: 100913970
FAB: 101816678
PHI: 102103763
PMAJ: 107199279
CLV: 106146061
ASN: 102386966
AMJ: 102574984
PSS: 102443605
CMY: 102946801
CPIC: 101954100
ACS: 100559713
PVT: 110074451
PBI: 103060795
GJA: 107119675
XLA: 108708601(cyp27b1.L) 108709871(cyp27b1.S)
XTR: 448754(cyp27b1)
NPR: 108791588
DRE: 793309(cyp27b1)
SRX: 107747831
SANH: 107684010
SGH: 107561793
CCAR: 109089182
IPU: 108254794
AMEX: 103035224
TRU: 101062641
NCC: 104955571
MZE: 101476393
OLA: 101172325
XMA: 102222483
PRET: 103465325
NFU: 107385653
CSEM: 103386049
LCF: 108898968
HCQ: 109522134
BPEC: 110171326
ELS: 105017033
LCM: 102346327
CMK: 103191415
SPU: 585633
 » show all
Taxonomy
Reference
1  [PMID:4404596]
  Authors
Gray RW, Omdahl JL, Ghazarian JG, DeLuca HF.
  Title
25-Hydroxycholecalciferol-1-hydroxylase. Subcellular location and properties.
  Journal
J Biol Chem 247:7528-32 (1972)
Reference
2  [PMID:10092858]
  Authors
Sakaki T, Sawada N, Takeyama K, Kato S, Inouye K
  Title
Enzymatic properties of mouse 25-hydroxyvitamin D3 1 alpha-hydroxylase expressed  in Escherichia coli.
  Journal
Eur J Biochem 259:731-8 (1999)
DOI:10.1046/j.1432-1327.1999.00096.x
Reference
3  [PMID:10518789]
  Authors
Sawada N, Sakaki T, Kitanaka S, Takeyama K, Kato S, Inouye K
  Title
Enzymatic properties of human 25-hydroxyvitamin D3 1alpha-hydroxylase coexpression with adrenodoxin and NADPH-adrenodoxin reductase in Escherichia coli.
  Journal
Eur J Biochem 265:950-6 (1999)
DOI:10.1046/j.1432-1327.1999.00794.x
Other DBs
ExplorEnz - The Enzyme Database: 1.14.15.18
IUBMB Enzyme Nomenclature: 1.14.15.18
ExPASy - ENZYME nomenclature database: 1.14.15.18
BRENDA, the Enzyme Database: 1.14.15.18
CAS: 9081-36-1

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