KEGG   ENZYME: 1.14.15.8Help
Entry
EC 1.14.15.8                Enzyme                                 

Name
steroid 15beta-monooxygenase;
cytochrome P-450meg;
cytochrome P450meg;
steroid 15beta-hydroxylase;
CYP106A2;
BmCYP106A2
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With reduced iron-sulfur protein as one donor, and incorporation of one atom of oxygen into the other donor
BRITE hierarchy
Sysname
progesterone,reduced-ferredoxin:oxygen oxidoreductase (15beta-hydroxylating)
Reaction(IUBMB)
progesterone + 2 reduced [2Fe-2S] ferredoxin + O2 = 15beta-hydroxyprogesterone + 2 oxidized [2Fe-2S] ferredoxin + H2O [RN:R09587]
Reaction(KEGG)
Substrate
progesterone [CPD:C00410];
reduced [2Fe-2S] ferredoxin;
O2 [CPD:C00007]
Product
15beta-hydroxyprogesterone [CPD:C19726];
oxidized [2Fe-2S] ferredoxin;
H2O [CPD:C00001]
Comment
The enzyme from the bacterium Bacillus megaterium hydroxylates a variety of 3-oxo-Delta4-steroids in position 15beta. Ring A-reduced, aromatic, and 3beta-hydroxy-Delta4-steroids do not serve as substrates [2].
History
EC 1.14.15.8 created 2010
Orthology
K21114  steroid 15beta-monooxygenase
Genes
BSJ: UP17_19080
Taxonomy
Reference
1  [PMID:109432]
  Authors
Berg A, Ingelman-Sundberg M, Gustafsson JA
  Title
Purification and characterization of cytochrome P-450meg.
  Journal
J Biol Chem 254:5264-71 (1979)
Reference
2  [PMID:177422]
  Authors
Berg A, Gustafsson JA, Ingelman-Sundberg M
  Title
Characterization of a cytochrome P-450-dependent steroid hydroxylase system present in Bacillus megaterium.
  Journal
J Biol Chem 251:2831-8 (1976)
Reference
3  [PMID:15178459]
  Authors
Lisurek M, Kang MJ, Hartmann RW, Bernhardt R
  Title
Identification of monohydroxy progesterones produced by CYP106A2 using comparative HPLC and electrospray ionisation collision-induced dissociation mass  spectrometry.
  Journal
Biochem Biophys Res Commun 319:677-82 (2004)
DOI:10.1016/j.bbrc.2004.05.037
  Sequence
Reference
4  [PMID:19635596]
  Authors
Goni G, Zollner A, Lisurek M, Velazquez-Campoy A, Pinto S, Gomez-Moreno C, Hannemann F, Bernhardt R, Medina M
  Title
Cyanobacterial electron carrier proteins as electron donors to CYP106A2 from Bacillus megaterium ATCC 13368.
  Journal
Biochim Biophys Acta 1794:1635-42 (2009)
DOI:10.1016/j.bbapap.2009.07.012
Reference
5  [PMID:18481342]
  Authors
Lisurek M, Simgen B, Antes I, Bernhardt R
  Title
Theoretical and experimental evaluation of a CYP106A2 low homology model and production of mutants with changed activity and selectivity of hydroxylation.
  Journal
Chembiochem 9:1439-49 (2008)
DOI:10.1002/cbic.200700670
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 1.14.15.8
IUBMB Enzyme Nomenclature: 1.14.15.8
ExPASy - ENZYME nomenclature database: 1.14.15.8
BRENDA, the Enzyme Database: 1.14.15.8

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