KEGG   ENZYME: 1.14.19.6Help
Entry
EC 1.14.19.6                Enzyme                                 

Name
acyl-CoA (9+3)-desaturase;
oleoyl-CoA 12-desaturase;
Delta12 fatty acid desaturase;
Delta12(omega6)-desaturase;
oleoyl-CoA Delta12 desaturase;
Delta12 desaturase;
Delta12-desaturase;
Delta12-fatty-acid desaturase;
acyl-CoA,hydrogen donor:oxygen Delta12-oxidoreductase
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With oxidation of a pair of donors resulting in the reduction of O2 to two molecules of water
BRITE hierarchy
Sysname
acyl-CoA,ferrocytochrome b5:oxygen oxidoreductase (12,13 cis-dehydrogenating)
Reaction(IUBMB)
(1) oleoyl-CoA + 2 ferrocytochrome b5 + O2 + 2 H+ = linoleoyl-CoA + 2 ferricytochrome b5 + 2 H2O [RN:R11043];
(2) palmitoleoyl-CoA + 2 ferrocytochrome b5 + O2 + 2 H+ = (9Z,12Z)-hexadeca-9,12-dienoyl-CoA + 2 ferricytochrome b5 + 2 H2O [RN:R11044]
Reaction(KEGG)
Substrate
oleoyl-CoA [CPD:C00510];
ferrocytochrome b5 [CPD:C00999];
O2 [CPD:C00007];
H+ [CPD:C00080];
palmitoleoyl-CoA [CPD:C21072]
Product
linoleoyl-CoA [CPD:C02050];
ferricytochrome b5 [CPD:C00996];
H2O [CPD:C00001];
(9Z,12Z)-hexadeca-9,12-dienoyl-CoA [CPD:C21000]
Comment
This microsomal enzyme introduces a cis double bond at position 12 of fatty-acyl-CoAs that contain a cis double bond at position 9. When acting on 19:1Delta10 fatty acyl-CoA the enzyme from the pathogenic protozoan Trypanosoma brucei introduces the new double bond at position 13, indicating that the new double bond is introduced three carbons from the existing cis double bond, towards the methyl-end of the fatty acid. Requires cytochrome b5 as the electron donor [4].
History
EC 1.14.19.6 created 2008, modified 2015
Pathway
ec01040  Biosynthesis of unsaturated fatty acids
Orthology
K10256  omega-6 fatty acid desaturase / acyl-lipid omega-6 desaturase (Delta-12 desaturase)
Genes
RSS: 109441170
EPA: 110243700
ADF: 107344154
ATH: AT3G12120(FAD2)
ALY: ARALYDRAFT_478568
CRB: 17890721
CSAT: 104745425 104764975 104776214
EUS: EUTSA_v10020910mg
BRP: 103870276(FAD2-1)
BNA: 106417584 106418116 106452409 106452523 106453262 111197729 111197774
BOE: 106295108
CPAP: 110811677
CIT: 102627239
GMX: 100780912(GMFAD2-2D) 100805040(GMFAD2-2B) 100805777(FAD2-1B) 100809290(GMFAD2-2A) 102662570 547814(FAD2-1A) 547815(FAD2-2) 732617(FAD2-3)
LJA: Lj1g3v4317650.1(Lj1g3v4317650.1) Lj5g3v2237610.1(Lj5g3v2237610.1) Lj5g3v2237610.2(Lj5g3v2237610.2)
ZJU: 107432351
CSV: 101222623
CMO: 103486770
MCHA: 111024845
RCU: 8267086
JCU: 105646514
POP: 7479530
VVI: 100252129
SIND: 105159686 105163532(FAD2)
OSA: 4330523
DOSA: Os02t0716500-01(Os02g0716500) Os07t0416700-00(Os07g0416700)
OBR: 102719739
ATS: 109756354(LOC109756354) 109767804(LOC109767804) 109768827(LOC109768827) 109774922(LOC109774922) 109774938(LOC109774938) 109781985(LOC109781985)
ZMA: 100282744
PDA: 103713931
EGU: 105050201
MUS: 103980416
ATR: 18439780
MNG: MNEG_7806
NCR: NCU02209(ods)
NTE: NEUTE1DRAFT86398(NEUTE1DRAFT_86398)
MGR: MGG_01985
SSCK: SPSK_00128
MAW: MAC_06628
ANI: AN1037.2
ANG: ANI_1_742074(An08g05160)
ABE: ARB_01637
TVE: TRV_05152
PTE: PTT_13901
CNE: CND02750
CNB: CNBD3600
MGL: MGL_1345
NGD: NGA_0081700(DES)
TCR: 429257.20
 » show all
Taxonomy
Reference
1  [PMID:2248971]
  Authors
Borgeson CE, de Renobales M, Blomquist GJ.
  Title
Characterization of the delta 12 desaturase in the American cockroach, Periplaneta americana: the nature of the substrate.
  Journal
Biochim Biophys Acta 1047:135-40 (1990)
DOI:10.1016/0005-2760(90)90039-Z
Reference
2  [PMID:8900454]
  Authors
Lomascolo A, Dubreucq E, Galzy P.
  Title
Study of the delta 12-desaturase system of Lipomyces starkeyi.
  Journal
Lipids 31:253-9 (1996)
DOI:10.1007/BF02529871
Reference
3  [PMID:9829122]
  Authors
Tocher DR, Leaver MJ, Hodgson PA.
  Title
Recent advances in the biochemistry and molecular biology of fatty acyl desaturases.
  Journal
Prog Lipid Res 37:73-117 (1998)
DOI:10.1016/S0163-7827(98)00005-8
Reference
4  [PMID:15009186]
  Authors
Petrini GA, Altabe SG, Uttaro AD
  Title
Trypanosoma brucei oleate desaturase may use a cytochrome b5-like domain in another desaturase as an electron donor.
  Journal
Eur J Biochem 271:1079-86 (2004)
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 1.14.19.6
IUBMB Enzyme Nomenclature: 1.14.19.6
ExPASy - ENZYME nomenclature database: 1.14.19.6
BRENDA, the Enzyme Database: 1.14.19.6

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