KEGG   ENZYME: 1.14.20.6Help
Entry
EC 1.14.20.6                Enzyme                                 

Name
flavonol synthase;
FLS (gene name)
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With 2-oxoglutarate as one donor, and the other dehydrogenated
BRITE hierarchy
Sysname
dihydroflavonol,2-oxoglutarate:oxygen oxidoreductase
Reaction(IUBMB)
a dihydroflavonol + 2-oxoglutarate + O2 = a flavonol + succinate + CO2 + H2O [RN:R07368]
Reaction(KEGG)
Substrate
dihydroflavonol [CPD:C15570];
2-oxoglutarate [CPD:C00026];
O2 [CPD:C00007]
Product
flavonol [CPD:C01495];
succinate [CPD:C00042];
CO2 [CPD:C00011];
H2O [CPD:C00001]
Comment
In addition to the desaturation of (2R,3R)-dihydroflavonols to flavonols, the enzyme from Citrus unshiu (satsuma mandarin) also has a non-specific activity that trans-hydroxylates the flavanones (2S)-naringenin and the unnatural (2R)-naringenin at C-3 to kaempferol and (2R,3R)-dihydrokaempferol, respectively [2]. Requires Fe2+.
History
EC 1.14.20.6 created 2004 as EC 1.14.11.23, transferred 2018 to EC 1.14.20.6
Pathway
ec00941  Flavonoid biosynthesis
ec01100  Metabolic pathways
ec01110  Biosynthesis of secondary metabolites
Orthology
K05278  flavonol synthase
Genes
RSS: 109442146 109442183 109442644 109442938
ATH: AT5G08640(FLS1) AT5G43935(FLS6) AT5G63590(FLS3) AT5G63595(FLS4) AT5G63600(FLS5)
ALY: ARALYDRAFT_321431 ARALYDRAFT_332580 ARALYDRAFT_487705 ARALYDRAFT_494607 ARALYDRAFT_496548 ARALYDRAFT_496549 ARALYDRAFT_667796 ARALYDRAFT_686072
CRB: 17874951 17874978 17875062 17875365 17875649 17876064 17876240 17876394 17876899 17884061 17887601
CSAT: 104708215 104725029 104726949 104726979 104726980 104726983 104726984 104726985 104726986 104727463 104729053 104734897 104741256 104741513 104741522 104741531 104741542 104741553 104741561 104741572 104741592 104741603 104762497 104762498 104762499 104762501 104762503 104769085 104778112 104784343 109124872
EUS: EUTSA_v10004662mg EUTSA_v10004668mg EUTSA_v10005710mg EUTSA_v10014054mg EUTSA_v10015952mg EUTSA_v10028827mg EUTSA_v10029534mg
BRP: 103837496 103847177 103855056 103855057 103873165 103873769 103873770
BNA: 106348278 106348289 106348297 106351881 106351882 106364152 106382906 106397936 106405224 106409586 106419433 106444537 111201386
BOE: 106313418 106316306 106320382 106328324 106332816 106341567
THJ: 104819356
CPAP: 110816514
LJA: Lj1g3v0705350.1(Lj1g3v0705350.1) Lj1g3v0775780.1(Lj1g3v0775780.1) Lj1g3v1380920.1(Lj1g3v1380920.1) Lj5g3v1865700.1(Lj5g3v1865700.1)
MDM: 103410752 103413102(FLS)
CSV: 101219245
CMO: 103488630
MCHA: 111011799
VVI: 100232938(FLS1) 100232939(FLS1) 100243852(FLS2) 100249002(FLS3) 100267819(FLS5)
SLY: 101249699
SPEN: 107004995
SOT: 102577717(FLS)
NTA: 107794305(FLS1) 107814657(NtFLS)
NSY: 104236078
NTO: 104101386
INI: 109175998
LSV: 111900153
OSA: 4330843
DOSA: Os02t0767300-01(Os02g0767300)
OBR: 102712047
BDI: 100831956
ATS: 109764595(LOC109764595) 109768154(LOC109768154) 109784747(LOC109784747)
SBI: 8078926
SITA: 101780580
PDA: 103712517
EGU: 105058232
PEQ: 110019794
AOF: 109820207
 » show all
Taxonomy
Reference
1  [PMID:12180990]
  Authors
Wellmann F, Lukacin R, Moriguchi T, Britsch L, Schiltz E, Matern U.
  Title
Functional expression and mutational analysis of flavonol synthase from Citrus unshiu.
  Journal
Eur J Biochem 269:4134-42 (2002)
DOI:10.1046/j.1432-1033.2002.03108.x
  Sequence
Reference
2  [PMID:12620339]
  Authors
Lukacin R, Wellmann F, Britsch L, Martens S, Matern U.
  Title
Flavonol synthase from Citrus unshiu is a bifunctional dioxygenase.
  Journal
Phytochemistry 62:287-92 (2003)
DOI:10.1016/S0031-9422(02)00567-8
Reference
3  [PMID:12782296]
  Authors
Martens S, Forkmann G, Britsch L, Wellmann F, Matern U, Lukacin R.
  Title
Divergent evolution of flavonoid 2-oxoglutarate-dependent dioxygenases in parsley.
  Journal
FEBS Lett 544:93-8 (2003)
DOI:10.1016/S0014-5793(03)00479-4
  Sequence
Reference
4  [PMID:14570878]
  Authors
Turnbull JJ, Nakajima J, Welford RW, Yamazaki M, Saito K, Schofield CJ.
  Title
Mechanistic studies on three 2-oxoglutarate-dependent oxygenases of flavonoid biosynthesis: anthocyanidin synthase, flavonol synthase, and flavanone 3beta-hydroxylase.
  Journal
J Biol Chem 279:1206-16 (2004)
DOI:10.1074/jbc.M309228200
Other DBs
ExplorEnz - The Enzyme Database: 1.14.20.6
IUBMB Enzyme Nomenclature: 1.14.20.6
ExPASy - ENZYME nomenclature database: 1.14.20.6
BRENDA, the Enzyme Database: 1.14.20.6
CAS: 146359-76-4

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