KEGG   ENZYME: 1.14.99.22Help
Entry
EC 1.14.99.22               Enzyme                                 

Name
ecdysone 20-monooxygenase;
alpha-ecdysone C-20 hydroxylase;
ecdysone 20-hydroxylase
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
Miscellaneous
BRITE hierarchy
Sysname
Ecdysone,hydrogen-donor:oxygen oxidoreductase (20-hydroxylating)
Reaction(IUBMB)
ecdysone + reduced acceptor + O2 = 20-hydroxyecdysone + acceptor + H2O [RN:R02374]
Reaction(KEGG)
Substrate
ecdysone [CPD:C00477];
reduced acceptor [CPD:C00030];
O2 [CPD:C00007]
Product
20-hydroxyecdysone [CPD:C02633];
acceptor [CPD:C00028];
H2O [CPD:C00001]
Comment
An enzyme from insect fat body or malpighian tubules involving a heme-thiolate protein (P-450). NADPH can act as ultimate hydrogen donor.
History
EC 1.14.99.22 created 1978
Pathway
ec00981  Insect hormone biosynthesis
Orthology
K10723  ecdysone 20-monooxygenase
Genes
DME: Dmel_CG13478(shd)
DPO: Dpse_GA12315
DAN: Dana_GF24608
DER: 6545923
DSE: Dsec_GM25470
DSI: Dsimw501_GD14493(Dsim_GD14493)
DWI: 6651961
DYA: Dyak_GE22018
DGR: Dgri_GH14751
DMO: Dmoj_GI11492
MDE: 101896979
AAG: 5574128
AME: 411057(Cyp314a1)
BIM: 100742793
BTER: 100649449
SOC: 105204717
PBAR: 105423368
HST: 105191056
DQU: 106752394
CFO: 105250706
LHU: 105677150
PGC: 109859613
PCF: 106787266
NVI: 100115247(CYP314A1)
MDL: 103568534
TCA: 661451(Cyp314a1)
DPA: 109533342
NVL: 108563010
BMOR: 100127102(Cyp314a1)
PMAC: 106715493
PRAP: 110995369
HAW: 110376828
PXY: 105393180
CLEC: 106668127
ZNE: 110832310
FCD: 110845053
TUT: 107372108
 » show all
Taxonomy
Reference
1  [PMID:606249]
  Authors
Johnson P, Rees HH.
  Title
The mechanism of C-20 hydroxylation of alpha-ecdysone in the desert locust, Schistocerca gregaria.
  Journal
Biochem J 168:513-20 (1977)
Reference
2  [PMID:5286]
  Authors
Nigg HN, Svoboda JA, Thompson MJ, Dutky SR, Kaplanis JN, Robbins WE.
  Title
Ecdysome 20-hydroxylase from the midgut of the tobacco hornworm (Manduca sexta L.).
  Journal
Experientia 32:438-9 (1976)
Reference
3  [PMID:488526]
  Authors
Smith SL, Bollenbacher WE, Cooper DY, Schleyer H, Wielgus JJ, Gilbert LI.
  Title
Ecdysone 20-monooxygenase: characterization of an insect cytochrome p-450 dependent steroid hydroxylase.
  Journal
Mol Cell Endocrinol 15:111-33 (1979)
Other DBs
ExplorEnz - The Enzyme Database: 1.14.99.22
IUBMB Enzyme Nomenclature: 1.14.99.22
ExPASy - ENZYME nomenclature database: 1.14.99.22
BRENDA, the Enzyme Database: 1.14.99.22
CAS: 55071-97-1

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