KEGG   ENZYME: 1.17.1.3Help
Entry
EC 1.17.1.3                 Enzyme                                 

Name
leucoanthocyanidin reductase;
leucocyanidin reductase
Class
Oxidoreductases;
Acting on CH or CH2 groups;
With NAD+ or NADP+ as acceptor
BRITE hierarchy
Sysname
(2R,3S)-catechin:NADP+ 4-oxidoreductase
Reaction(IUBMB)
(2R,3S)-catechin + NADP+ + H2O = 2,3-trans-3,4-cis-leucocyanidin + NADPH + H+ [RN:R06532]
Reaction(KEGG)
R06532;
(other) R06533 R06615
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Substrate
(2R,3S)-catechin [CPD:C06562];
NADP+ [CPD:C00006];
H2O [CPD:C00001]
Product
2,3-trans-3,4-cis-leucocyanidin [CPD:C05906];
NADPH [CPD:C00005];
H+ [CPD:C00080]
Comment
The enzyme catalyses the synthesis of catechin, catechin-4beta-ol (leucocyanidin) and the related flavan-3-ols afzelechin and gallocatechin, which are initiating monomers in the synthesis of plant polymeric proanthocyanidins or condensed tannins. While 2,3-trans-3,4-cis-leucocyanidin is the preferred flavan-3,4-diol substrate, 2,3-trans-3,4-cis-leucodelphinidin and 2,3-trans-3,4-cis-leucopelargonidin can also act as substrates, but more slowly. NADH can replace NADPH but is oxidized more slowly.
History
EC 1.17.1.3 created 2003
Pathway
ec00941  Flavonoid biosynthesis
ec01110  Biosynthesis of secondary metabolites
Orthology
K13081  leucoanthocyanidin reductase
Genes
CPAP: 110824010
CIT: 102621411
CIC: CICLE_v10033821mg
TCC: 18604056(LAR) 18610545
GRA: 105764571(lar1) 105765625(lar2)
GHI: 107931042 107934794 107946451
DZI: 111290788 111316766 111318411
EGR: 104426102 104426103 104433307
GMX: 100786879(LAR1) 100795100
PVU: PHAVU_007G102100g
VRA: 106771362
VAR: 108324973
CCAJ: 109813851
CAM: 101493305
LJA: Lj2g3v1172450.1(Lj2g3v1172450.1)
ADU: 107491282
AIP: 107643784
LANG: 109356072
FVE: 101306809
PPER: 18789589
PMUM: 103341429
RCU: 8269513
JCU: 105641976
VVI: 100232982(LAR1) 100233112(LAR2)
SOT: 102594738
NSY: 104222673
NTO: 104107265
OEU: 111369334
HAN: 110917901
BVG: 104908227
SOE: 110789492
OSA: 4332311
DOSA: Os03t0259400-00(Os03g0259400)
OBR: 102717501
BDI: 100839668
ATS: 109751108(LOC109751108)
MUS: 103974395
AOF: 109834931
ATR: 18423736
 » show all
Taxonomy
Reference
1  [PMID:8470799]
  Authors
Tanner GJ, Kristiansen KN.
  Title
Synthesis of 3,4-cis-[3H]leucocyanidin and enzymatic reduction to catechin.
  Journal
Anal Biochem 209:274-7 (1993)
DOI:10.1006/abio.1993.1119
Reference
2  [PMID:12788945]
  Authors
Tanner GJ, Francki KT, Abrahams S, Watson JM, Larkin PJ, Ashton AR.
  Title
Proanthocyanidin biosynthesis in plants. Purification of legume leucoanthocyanidin reductase and molecular cloning of its cDNA.
  Journal
J Biol Chem 278:31647-56 (2003)
DOI:10.1074/jbc.M302783200
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 1.17.1.3
IUBMB Enzyme Nomenclature: 1.17.1.3
ExPASy - ENZYME nomenclature database: 1.17.1.3
BRENDA, the Enzyme Database: 1.17.1.3
CAS: 776323-46-7

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