KEGG   ENZYME: 1.17.2.1Help
Entry
EC 1.17.2.1                 Enzyme                                 

Name
nicotinate dehydrogenase (cytochrome);
nicotinic acid hydroxylase;
nicotinate hydroxylase
Class
Oxidoreductases;
Acting on CH or CH2 groups;
With a cytochrome as acceptor
BRITE hierarchy
Sysname
nicotinate:cytochrome 6-oxidoreductase (hydroxylating)
Reaction(IUBMB)
nicotinate + a ferricytochrome + H2O = 6-hydroxynicotinate + a ferrocytochrome + 2 H+ [RN:R09473]
Reaction(KEGG)
Substrate
nicotinate [CPD:C00253];
ferricytochrome [CPD:C00923];
H2O [CPD:C00001]
Product
6-hydroxynicotinate [CPD:C01020];
ferrocytochrome [CPD:C00924];
H+ [CPD:C00080]
Comment
This two-component enzyme from Pseudomonas belongs to the family of xanthine dehydrogenases, but differs from most other members of this family. While most members contain an FAD cofactor, the large subunit of this enzyme contains three c-type cytochromes, enabling it to interact with the electron transfer chain, probably by delivering the electrons to a cytochrome oxidase. The small subunit contains a typical molybdopterin cytosine dinucleotide(MCD) cofactor and two [2Fe-2S] clusters [1].
History
EC 1.17.2.1 created 2010
Pathway
ec00760  Nicotinate and nicotinamide metabolism
ec01120  Microbial metabolism in diverse environments
Orthology
K18029  nicotinate dehydrogenase subunit A
K18030  nicotinate dehydrogenase subunit B
Genes
GQU: AWC35_02960 AWC35_02965
SPE: Spro_2314 Spro_2315
SRR: SerAS9_2271 SerAS9_2272
SRL: SOD_c21440(nicA) SOD_c21450(nicB1)
SRY: M621_11745 M621_11750
SPLY: Q5A_011880(nicA) Q5A_011885(nicB)
SRS: SerAS12_2271 SerAS12_2272
SRA: SerAS13_2272 SerAS13_2273
SMAF: D781_1723
SMW: SMWW4_v1c23280 SMWW4_v1c23290 SMWW4_v1c24890
PHEI: NCTC12003_00963(iorA)
PAE: PA1602
PAEV: N297_1647
PAEI: N296_1647
PAEP: PA1S_18005
PAEM: U769_17735
PAEL: T223_19065
PAEG: AI22_15975
PAEC: M802_1644
PAEO: M801_1646
PPU: PP_3947(nicA) PP_3948(nicB)
PSEM: TO66_20980
RIN: ACS15_2998(nicA)
REH: H16_A0920(h16_A0920) H16_A0921(h16_A0921)
CNC: CNE_1c08700(adhB1) CNE_1c08710(iorA1)
CGD: CR3_3011(nicA)
BUB: BW23_4786
BPE: BP1953
BPC: BPTD_1925
BPER: BN118_0961
BPEU: Q425_14990
BPA: BPP2326
BPAR: BN117_1480
BAV: BAV1533(iorA) BAV1534
AXX: ERS451415_03303(adhB_1) ERS451415_03304(iorA_1)
AFQ: AFA_14980(nicA) AFA_14985(nicB2)
POL: Bpro_4058
LIM: L103DPR2_00173(nicA_1) L103DPR2_00692(nicA_2)
CCX: COCOR_06361(iorA2)
ARA: Arad_8291 Arad_8292(xdhA)
MAQU: Maq22A_1p31265(coxL) Maq22A_1p38300(coxS)
HDI: HDIA_4148(nicA_1) HDIA_4442(nicA_2) HDIA_4443(nicB_3)
MAGX: XM1_1926(pucE)
MAGN: WV31_17390
OBG: Verru16b_02806(nicA_2)
AALG: AREALGSMS7_00647(nicA)
 » show all
Taxonomy
Reference
1  [PMID:18678916]
  Authors
Jimenez JI, Canales A, Jimenez-Barbero J, Ginalski K, Rychlewski L, Garcia JL, Diaz E
  Title
Deciphering the genetic determinants for aerobic nicotinic acid degradation: the  nic cluster from Pseudomonas putida KT2440.
  Journal
Proc Natl Acad Sci U S A 105:11329-34 (2008)
DOI:10.1073/pnas.0802273105
  Sequence
Reference
2  [PMID:19118407]
  Authors
Yang Y, Yuan S, Chen T, Ma P, Shang G, Dai Y
  Title
Cloning, heterologous expression, and functional characterization of the nicotinate dehydrogenase gene from Pseudomonas putida KT2440.
  Journal
Biodegradation 20:541-9 (2009)
DOI:10.1007/s10532-008-9243-x
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 1.17.2.1
IUBMB Enzyme Nomenclature: 1.17.2.1
ExPASy - ENZYME nomenclature database: 1.17.2.1
BRENDA, the Enzyme Database: 1.17.2.1

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