KEGG   ENZYME: 1.18.6.1Help
Entry
EC 1.18.6.1                 Enzyme                                 

Name
nitrogenase;
reduced ferredoxin:dinitrogen oxidoreductase (ATP-hydrolysing)
Class
Oxidoreductases;
Acting on iron-sulfur proteins as donors;
With dinitrogen as acceptor
BRITE hierarchy
Sysname
ferredoxin:dinitrogen oxidoreductase (ATP-hydrolysing, molybdenum-dependent)
Reaction(IUBMB)
8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O = 8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate [RN:R05185]
Reaction(KEGG)
Substrate
reduced ferredoxin [CPD:C00138];
H+ [CPD:C00080];
N2 [CPD:C00697];
ATP [CPD:C00002];
H2O [CPD:C00001]
Product
oxidized ferredoxin [CPD:C00139];
H2 [CPD:C00282];
NH3 [CPD:C00014];
ADP [CPD:C00008];
phosphate [CPD:C00009]
Comment
Requires Mg2+. The enzyme is a complex of two components (namely dinitrogen reductase and dinitrogenase). Dinitrogen reductase is a [4Fe-4S] protein, which, in the presence of two molecules of ATP, transfers an electron from ferredoxin to the dinitrogenase component. Dinitrogenase is a molybdenum-iron protein that reduces dinitrogen to two molecules of ammonia in three successive two-electron reductions via diazene and hydrazine. The reduction is initiated by formation of hydrogen in stoichiometric amounts [2]. Acetylene is reduced to ethylene (but only very slowly to ethane), azide to nitrogen and ammonia, and cyanide to methane and ammonia. In the absence of a suitable substrate, hydrogen is slowly formed. Ferredoxin may be replaced by flavodoxin [see EC 1.19.6.1 nitrogenase (flavodoxin)]. The enzyme does not reduce CO (cf. EC 1.18.6.2, vanadium-dependent nitrogenase).
History
EC 1.18.6.1 created 1978 as EC 1.18.2.1, transferred 1984 to EC 1.18.6.1, modified 2005, modified 2018
Pathway
ec00625  Chloroalkane and chloroalkene degradation
ec00910  Nitrogen metabolism
ec01100  Metabolic pathways
ec01120  Microbial metabolism in diverse environments
Orthology
K00531  nitrogenase delta subunit
K02586  nitrogenase molybdenum-iron protein alpha chain
K02591  nitrogenase molybdenum-iron protein beta chain
Genes
ENR: H650_03215 H650_03220
ENF: AKI40_3044 AKI40_3045
KPV: KPNIH29_17530 KPNIH29_17535
KPE: KPK_1712(nifK) KPK_1713(nifD)
KVA: Kvar_1601 Kvar_1602
KPK: A593_02120 A593_02125
KVD: KR75_26125 KR75_26130
KVQ: SP68_06270 SP68_06275
KOX: KOX_24925 KOX_24930
KOE: A225_3836 A225_3837
KQV: B8P98_09570(nifK) B8P98_09575(nifD)
KRD: A3780_10720(nifK) A3780_10725(nifD) A3780_13145(anfK) A3780_13150(anfG) A3780_13155(anfD)
EBC: C2U52_22725(nifK) C2U52_22730(nifD)
SERA: Ser39006_005335(nifD) Ser39006_005340(nifK)
ECA: ECA2954(nifK) ECA2955(nifD)
PATO: GZ59_29580(nifK) GZ59_29590(nifD)
DDD: Dda3937_02156(nifD) Dda3937_02157(nifK)
DFN: CVE23_19775(nifK) CVE23_19780(nifD)
PSA: PST_1327(nifD) PST_1328(nifK)
PSR: PSTAA_1359(nifD) PSTAA_1360(nifK)
AVN: Avin_01390(nifD) Avin_01400(nifK) Avin_48970(anfK) Avin_48980(anfG) Avin_48990(anfD)
AVL: AvCA_01390(nifD) AvCA_01400(nifK) AvCA_48970(anfK) AvCA_48980(anfG) AvCA_48990(anfD)
AVD: AvCA6_01390(nifD) AvCA6_01400(nifK) AvCA6_48970(anfK) AvCA6_48980(anfG) AvCA6_48990(anfD)
ACX: Achr_1270(nifD) Achr_1280(nifK)
TTU: TERTU_1538(nifD) TERTU_1539(nifK)
MCA: MCA0230(nifD) MCA0231(nifK)
MPSY: CEK71_01055(nifD) CEK71_01060(nifK)
ACII: C4901_14740(nifK) C4901_14745(nifD)
CTI: pRALTA_0391(nifD) pRALTA_0392(nifK)
BVE: AK36_5493(nifD) AK36_5494(nifK)
BXE: Bxe_B1466(nifD) Bxe_B1467(nifK)
BXB: DR64_6773(nifD) DR64_6774(nifK)
PARB: CJU94_32990(nifD) CJU94_36080(nifK)
HSE: Hsero_2851(nifK) Hsero_2852(nifD)
HRB: Hrubri_2375(nifK) Hrubri_2376(nifD)
RGE: RGE_25450(nifK) RGE_42890(nifD) RGE_42900(nifK)
METR: BSY238_1222(nifD) BSY238_1223(nifK)
DEY: HYN24_06700(nifK) HYN24_06705(nifD)
AZO: azo0539(nifD) azo0540(nifK)
AZA: AZKH_0789(nifK) AZKH_0790(nifD) AZKH_4079(nifD) AZKH_4080(nifK)
ACOM: CEW83_10465(nifK) CEW83_10470(nifD)
ZPA: C3497_06300(nifK) C3497_06305(nifD)
WSU: WS1391(NIFK) WS1392(NIFD)
SMUL: SMUL_1287(nifD) SMUL_1288(nifK) SMUL_1652(anfK) SMUL_1653(anfG) SMUL_1654(anfD)
GSU: GSU2819(nifK) GSU2820(nifD)
GSK: KN400_2758(nifK) KN400_2759(nifD)
GME: Gmet_0663(nifD) Gmet_0664(nifK)
GBM: Gbem_2075(nifD) Gbem_2076(nifK)
PCA: Pcar_2099(nifD) Pcar_2100(nifK)
DES: DSOUD_2560(nifK) DSOUD_2561(nifD)
DVU: DVUA0011(nifK) DVUA0012(nifD)
DMA: DMR_20520(nifK) DMR_20530(nifD)
DPI: BN4_20071(nifD) BN4_20072(nifK)
PPRF: DPRO_3406(nifD) DPRO_3407(nifK)
DAT: HRM2_09700(nifD2) HRM2_09710(nifK1)
SME: SMa0827(nifD) SMa0829(nifK)
SMX: SM11_pC1123(nifK) SM11_pC1124(nifD)
SMI: BN406_04238(nifD1) BN406_04239(nifK1)
SMEL: SM2011_a0827(nifD) SM2011_a0829(nifK)
RHI: NGR_a00870(nifK2) NGR_a00880(nifD2) NGR_a01110(nifK1) NGR_a01120(nifD1)
REL: REMIM1_PE00301(nifK) REMIM1_PE00302(nifD)
REP: IE4803_PB00349(nifK-1) IE4803_PB00350(nifD-1) IE4803_PB00460(nifK-2) IE4803_PB00461(nifD-2)
REI: IE4771_PB00216(nifD) IE4771_PB00217(nifK)
RLE: pRL100160(nifK) pRL100161(nifD)
RIR: BN877_p0283(nifK) BN877_p0284(nifD)
RGA: RGR602_PB00284(nifD) RGR602_PB00285(nifK)
RHN: AMJ98_PC00260(nifK-1) AMJ98_PC00261(nifD-1) AMJ98_PC00355(nifK-2) AMJ98_PC00356(nifD-2)
RPHA: AMC79_PC00237(nifK-1) AMC79_PC00238(nifD-1) AMC79_PC00326(nifK-2) AMC79_PC00327(nifD-2)
RHX: AMK02_PC00255(nifK-1) AMK02_PC00256(nifD-1) AMK02_PC00344(nifK-2) AMK02_PC00345(nifD-2)
RHK: Kim5_PA00322(nifK-1) Kim5_PA00323(nifD-1) Kim5_PA00449(nifK-2) Kim5_PA00450(nifD-2)
NGL: RG1141_PB00680(nifD) RG1141_PB00690(nifK)
NGG: RG540_PA10430(nifD) RG540_PA10440(nifK)
BJA: blr1743(nifD) blr1744(nifK)
BJU: BJ6T_80750(nifK) BJ6T_80760(nifD)
BRA: BRADO5438(nifK) BRADO5439(nifD)
BBT: BBta_5923(nifK) BBta_5924(nifD)
BRS: S23_46420(nifK) S23_46430(nifD)
AOL: S58_22670(nifD) S58_22680(nifK)
BRO: BRAD285_1796(nifD) BRAD285_1797(nifK)
BOT: CIT37_04870(nifK) CIT37_04875(nifD)
RPA: RPA1435(anfK) RPA1436(anfG) RPA1437(anfD) RPA2348 RPA2363(NifD1) RPA2617 RPA2636(NifD2) RPA4618(nifK) RPA4619(nifD)
AZC: AZC_1039(nifK) AZC_1040(nifD)
HMC: HYPMC_3685(nifK) HYPMC_3686(nifD)
MSC: BN69_2624(nifD) BN69_2625
HDI: HDIA_4341(nifK1) HDIA_4342(nifD)
RSP: RSP_0539(nifK) RSP_0540(nifD)
SAGU: CDO87_16530(nifK) CDO87_16535(nifD)
GDI: GDI0437(nifD) GDI0438(nifK)
RCE: RC1_3681(nifK) RC1_3682(nifD)
MGY: MGMSRv2__0343(nifK) MGMSRv2__0344(nifD)
MAGX: XM1_3939(nifD) XM1_3940(nifK)
AZL: AZL_007690(nifK) AZL_007700(nifD)
ALI: AZOLI_0679(nifK) AZOLI_0680(nifD)
ABS: AZOBR_70126(nifK) AZOBR_70127(nifD)
AZT: TSH58p_07640(nifD) TSH58p_07645(nifK)
AZM: DM194_02550(nifK) DM194_02555(nifD)
NAO: Y958_05335(nifD) Y958_05340(nifK)
AFR: AFE_1520(nifK) AFE_1521(nifD)
PDH: B9T62_25635(nifK) B9T62_25640(nifD)
KYR: CVV65_08115(nifD) CVV65_08120(nifK)
CAC: CA_C0256(nifD) CA_C0257(nifK)
CAE: SMB_G0261(nifD) SMB_G0262(nifK)
CAY: CEA_G0262(nifD) CEA_G0263(nifK)
CKL: CKL_0370(anfD) CKL_0371(anfG) CKL_0372(anfK) CKL_0825(nifD1) CKL_0826(nifK1) CKL_1034(nifD2) CKL_1035(nifK2) CKL_1042(nifD3) CKL_1043(nifD4) CKL_1044(nifK3) CKL_1048(nifK4) CKL_3077(nifK7) CKL_3078(nifD10)
CLJ: CLJU_c04930(nifD1) CLJU_c04940(nifK1) CLJU_c23510(nifK2) CLJU_c23520(nifD2)
CSR: Cspa_c14120(nifE1) Cspa_c14130(nifK1) Cspa_c14150(nifD1) Cspa_c14160(nifK2) Cspa_c30780(nifD2) Cspa_c30790(nifK3) Cspa_c30880(anfD) Cspa_c30890(anfG) Cspa_c30900(anfK)
CACE: CACET_c14000(nifD) CACET_c14010(nifK1)
CCE: Ccel_1614
CSS: Cst_c19800(nifE) Cst_c19810(nifK)
BFI: CIY_11720
DED: DHBDCA_p2004(nifK) DHBDCA_p2005(nifD)
DEC: DCF50_p2016(nifK) DCF50_p2017(nifD)
HMO: HM1_0864(nifK) HM1_0865(nifD)
AWO: Awo_c06960(anfK) Awo_c06970(anfG) Awo_c06980(anfD) Awo_c07900(nifK) Awo_c07910(nifD)
MTA: Moth_0551
TPZ: Tph_c08680(nifD1) Tph_c08690(nifK)
CAD: Curi_c21590(nifK1) Curi_c21600(nifE3) Curi_c21650(nifK2)
SRI: SELR_27780(nifK) SELR_27790(nifE)
FAL: FRAAL6811(nifK) FRAAL6812(nifD)
CYA: CYA_1821(nifK) CYA_1823(nifD)
CYB: CYB_0422(nifD) CYB_0423(nifK)
LET: O77CONTIG1_04721(nifK1) O77CONTIG1_04722(nifD_2)
CYT: cce_0560(nifD) cce_0561(nifK)
ANA: all1440(nifK) all1454(nifD)
NOE: CLI64_00360(nifD) CLI64_00480(nifK)
ANB: ANA_C13511(nifD) ANA_C13533(nifK)
NSP: BMF81_04093(nifK) BMF81_04097(nifD)
CEO: ETSB_1470(nifK) ETSB_1471(nifD)
DET: DET1154(nifK) DET1155(nifD)
MIN: Minf_1873(nifD) Minf_1874(nifD)
EPO: Epro_0685(nifD) Epro_0686(nifK)
CTE: CT1536(nifD) CT1537(nifK)
PROC: Ptc2401_01795(nifK_2) Ptc2401_01796(nifD_2)
PROS: CHL67_07910(nifD) CHL67_07915(nifK)
TAL: Thal_0425
TYE: THEYE_A1715(nifK) THEYE_A1716(nifD)
LFC: LFE_2397(nifD) LFE_2398(nifK)
MMP: MMP0856(nifD) MMP0857(nifK)
MMG: MTBMA_c01490(nifD) MTBMA_c01500(nifK1) MTBMA_c01520(nifN)
MWO: MWSIV6_0104(nifD3) MWSIV6_0105(nifD5) MWSIV6_0106(nifD7) MWSIV6_0107(nifK1)
METE: tca_01512(vnfD) tca_01513(nifK_1) tca_01515(nifK_2)
MFC: BRM9_0192(nifN) BRM9_0194(nifK) BRM9_0195(nifD)
MCUB: MCBB_1935(nifK2) MCBB_1937(nifK) MCBB_1938(nifD)
MAC: MA_1208(anfK) MA_1209(anfG) MA_1210(anfD) MA_3898(nifD) MA_3899(nifK)
MPY: Mpsy_1344(nifD) Mpsy_1345(nifK)
MCJ: MCON_0610(nifD2) MCON_0611(nifK)
MEZ: Mtc_0145(nifD) Mtc_0146(nifK)
RCI: RCIX1611(nifD) RCIX1613(nifK)
 » show all
Taxonomy
Reference
1  [PMID:4390523]
  Authors
Zumft WG, Paneque A, Aparicio PJ, Losada M.
  Title
Mechanism of nitrate reduction in Chlorella.
  Journal
Biochem Biophys Res Commun 36:980-6 (1969)
DOI:10.1016/0006-291X(69)90300-3
Reference
2  [PMID:3200830]
  Authors
Liang J, Burris RH.
  Title
Hydrogen burst associated with nitrogenase-catalyzed reactions.
  Journal
Proc Natl Acad Sci U S A 85:9446-50 (1988)
DOI:10.1073/pnas.85.24.9446
Reference
3  [PMID:15382920]
  Authors
Dance I.
  Title
The mechanism of nitrogenase. Computed details of the site and geometry of binding of alkyne and alkene substrates and intermediates.
  Journal
J Am Chem Soc 126:11852-63 (2004)
DOI:10.1021/ja0481070
Reference
4  [PMID:10852721]
  Authors
Chan JM, Wu W, Dean DR, Seefeldt LC.
  Title
Construction and characterization of a heterodimeric iron protein: defining roles for adenosine triphosphate in nitrogenase catalysis.
  Journal
Biochemistry 39:7221-8 (2000)
DOI:10.1021/bi000219q
Other DBs
ExplorEnz - The Enzyme Database: 1.18.6.1
IUBMB Enzyme Nomenclature: 1.18.6.1
ExPASy - ENZYME nomenclature database: 1.18.6.1
UM-BBD (Biocatalysis/Biodegradation Database): 1.18.6.1
BRENDA, the Enzyme Database: 1.18.6.1
CAS: 9013-04-1

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