KEGG   ENZYME: 1.2.3.3Help
Entry
EC 1.2.3.3                  Enzyme                                 

Name
pyruvate oxidase;
pyruvic oxidase;
phosphate-dependent pyruvate oxidase
Class
Oxidoreductases;
Acting on the aldehyde or oxo group of donors;
With oxygen as acceptor
BRITE hierarchy
Sysname
pyruvate:oxygen 2-oxidoreductase (phosphorylating)
Reaction(IUBMB)
pyruvate + phosphate + O2 = acetyl phosphate + CO2 + H2O2 [RN:R00207]
Reaction(KEGG)
Substrate
pyruvate [CPD:C00022];
phosphate [CPD:C00009];
O2 [CPD:C00007]
Product
acetyl phosphate [CPD:C00227];
CO2 [CPD:C00011];
H2O2 [CPD:C00027]
Comment
A flavoprotein (FAD) requiring thiamine diphosphate. Two reducing equivalents are transferred from the resonant carbanion/enamine forms of 2-hydroxyethyl-thiamine-diphosphate to the adjacent flavin cofactor, yielding 2-acetyl-thiamine diphosphate (AcThDP) and reduced flavin. FADH2 is reoxidized by O2 to yield H2O2 and FAD and AcThDP is cleaved phosphorolytically to acetyl phosphate and thiamine diphosphate [2].
History
EC 1.2.3.3 created 1961
Pathway
ec00620  Pyruvate metabolism
ec01100  Metabolic pathways
Orthology
K00158  pyruvate oxidase
Genes
PRW: PsycPRwf_1595
PSYY: DLE54_07365(spxB)
RBS: RHODOSMS8_03471(ydaP)
LAP: ACP90_05560
LAGG: B0E33_17115
LABR: CHH27_15080
SULZ: C1J03_11960
GBE: GbCGDNIH1_2349
TII: DY252_15040
BSU: BSU04340(ydaP)
BSR: I33_0498
BSL: A7A1_1621
BSH: BSU6051_04340(ydaP)
BSUS: Q433_02560
BSS: BSUW23_02245(ydaP)
BST: GYO_0652
BSO: BSNT_06775(ydaP)
BSQ: B657_04340(ydaP)
BSX: C663_0428(ydaP)
BLI: BL00146(ydaP)
BLD: BLi00520(ydaP)
BLH: BaLi_c05450(ydaP)
BAY: RBAM_004560(ydaP)
BAQ: BACAU_0392(ydaP)
BYA: BANAU_0393(ydaP)
BAMP: B938_02060
BAML: BAM5036_0416(ydaP)
BAMA: RBAU_0430(ydaP)
BAMN: BASU_0415(ydaP)
BAMB: BAPNAU_0398(ydaP)
BAMT: AJ82_02515
BAMY: V529_04190(ydaP)
BMP: NG74_00436(ydaP)
BAO: BAMF_0400(ydaP)
BAZ: BAMTA208_02025(ydaP)
BQL: LL3_00418(ydaP)
BXH: BAXH7_00421(ydaP)
BQY: MUS_0426(ydaP)
BAMI: KSO_017400
BAMC: U471_04260
BAMF: U722_02320
BCE: BC2328
BCA: BCE_2426
BCQ: BCQ_2322(ydaP)
BNC: BCN_2316
BCER: BCK_22940
BTT: HD73_2612
BTHI: BTK_12960
BTM: MC28_1598
BTG: BTB_c24080(ydaP)
BWW: bwei_2641(ydaP)
BMYO: BG05_3665
BMYC: DJ92_5004
BPU: BPUM_0407
BPUM: BW16_02430
BPUS: UP12_02390
BJS: MY9_0454
BACW: QR42_02460
BACP: SB24_07535
BACB: OY17_04930
BACY: QF06_01145
BACL: BS34A_04970(ydaP)
BALM: BsLM_0458
BEO: BEH_15760
BGY: BGLY_0531(ydaP)
OIH: OB3409
HHD: HBHAL_2802(ydaP)
SAU: SA2327
SAV: SAV2539
SAW: SAHV_2523
SAM: MW2460
SAS: SAS2425
SAR: SAR2619
SAC: SACOL2553
SAX: USA300HOU_2531(ilvB2)
SAA: SAUSA300_2477(cidC)
SAE: NWMN_2438(poxB)
SAD: SAAV_2605
SUJ: SAA6159_02435(cidC)
SUK: SAA6008_02576(cidC)
SUZ: MS7_2544
SUW: SATW20_26600(cidC)
SUG: SAPIG2589
SUF: SARLGA251_23140(cidC)
SAUA: SAAG_00360
SAUE: RSAU_002380(cidC)
SAUS: SA40_2291(cidC)
SAUU: SA957_2375(cidC)
SAUG: SA268_2453(cidC)
SAUZ: SAZ172_2636(cidC)
SAUF: X998_2521
SAB: SAB2413c
SUY: SA2981_2475(cidC)
SAUB: C248_2596
SAUC: CA347_2613
SAUR: SABB_01144
SAUI: AZ30_13305
SAUD: CH52_06305
SAMS: NI36_12695
SEP: SE2103
SER: SERP2115
SEPP: SEB_02108
SEPS: DP17_1043
SHA: SH0519
SHH: ShL2_00415(cidC)
SSP: SSP0333
SCA: SCA_1991
SLN: SLUG_04500(cidC)
SDT: SPSE_0879
SPAS: STP1_1039
SXO: SXYL_00367(cidC)
SCAP: AYP1020_1708(ydaP)
SSCH: LH95_04350
SSCZ: RN70_04560
SAGQ: EP23_05540
MCAK: MCCS_01760(ydaP)
LMO: lmo0722
LMOE: BN418_0836
LMOD: LMON_0727
LMOW: AX10_12160
LMOQ: LM6179_1033(ydaP)
LMR: LMR479A_0740(ydaP)
LMOM: IJ09_06715
LMOG: BN389_07680(ydaP)
LMP: MUO_03955
LMOX: AX24_00970
LMQ: LMM7_0758(poxB)
LMS: LMLG_2904
LMOK: CQ02_03880
LIN: lin0730
LWE: lwe0691
LSG: lse_0637
LIV: LIV_0665
PSWU: SY83_20705
SIV: SSIL_1781
LLA: L0199(poxL)
LLK: LLKF_2290(poxL)
LLT: CVCAS_2031(poxL)
LLS: lilo_2033(poxL)
LLX: NCDO2118_2152(poxL)
LLM: llmg_2321(poxL)
LLW: kw2_2096
LLJ: LG36_1995(poxL)
LGR: LCGT_0941
LGV: LCGL_0962
LRN: CMV25_01165(spxB)
SPN: SP_0730(spxB)
SPD: SPD_0636(spxB)
SPR: spr0642(spxB)
SPW: SPCG_0679(spxB)
SJJ: SPJ_0670(spxB)
SNV: SPNINV200_06450(spxB)
SPX: SPG_0663(spxB)
SNT: SPT_0746(spxB)
SND: MYY_0766
SPNN: T308_03420
SNE: SPN23F06550(spxB)
SPV: SPH_0817(spxB)
SNC: HMPREF0837_11014(spxB)
SNM: SP70585_0777(spxB)
SPP: SPP_0741(spxB)
SNI: INV104_06090(spxB)
SPNG: HMPREF1038_00746(spxB)
SNB: SP670_0779(spxB)
SNP: SPAP_0706
SNX: SPNOXC06620(spxB)
SNU: SPNA45_01061(spxB)
SPNE: SPN034156_17110(spxB)
SPNU: SPN034183_06630(spxB)
SPNM: SPN994038_06520(spxB)
SPNO: SPN994039_06530(spxB)
SSA: SSA_0391(spxB)
SGO: SGO_0292(spxB)
SDS: SDEG_1198
SDC: SDSE_1172(poxL) SDSE_1174(poxL)
SMB: smi_1431(spxB)
SOR: SOR_1397(spxB)
SCP: HMPREF0833_11194(spxB)
SCF: Spaf_1788(poxL)
SSAH: HSISS4_00987(ilvB1)
SANG: SAIN_1574(spxB)
SANC: SANR_1810(pox1)
SANS: DK43_01595
SCG: SCI_0284(pox1)
SCON: SCRE_0264(pox1)
SCOS: SCR2_0264(pox1)
STRN: SNAG_1365(pox5)
LPL: lp_0849(pox1) lp_0852(pox2) lp_2629(pox3) lp_3587(pox4) lp_3589(pox5)
LPT: zj316_0188(pox4) zj316_0190(pox5) zj316_0899(pox1) zj316_0901(pox2) zj316_2532(pox3)
LPS: LPST_C0666(pox1) LPST_C0667(pox2) LPST_C2161(pox3) LPST_C2931(pox4) LPST_C2933(pox5)
LJO: LJ_1853
LJF: FI9785_1805(pox)
LJH: LJP_1790c
LAC: LBA1974(poxB)
LAD: LA14_1962
LAF: SD55_1962(poxB)
LSA: LCA_1188(pox1) LCA_1830(pox2)
LDB: Ldb2213(pox1)
LBU: LBUL_2034
LBR: LVIS_0313
LCB: LCABL_05020(cidC) LCABL_20020(poxL) LCABL_23230(poxF)
LCW: BN194_05070(ydaP) BN194_19670(pox5) BN194_22810(pox5_2)
LGA: LGAS_1893
LHL: LBHH_0457(poxF)
LRH: LGG_00525(cidC) LGG_01836(spxB) LGG_02145(spxB)
LRL: LC705_00518(cidC) LC705_01818(spxB) LC705_02152(spxB)
LRA: LRHK_1811(spxB) LRHK_2153(spxB) LRHK_534
LCR: LCRIS_02019(poxL)
LAM: LA2_10765
LAE: LBAT_1650
PCE: PECL_1593(spxB) PECL_734(spxB)
EFU: HMPREF0351_10017(poxB)
EFM: M7W_239
EHR: EHR_04795
ECAS: ECBG_02190
EMU: EMQU_0017
EDU: LIU_01215
EGA: AL523_12185(spxB)
ETH: CK496_00090(spxB)
MPS: MPTP_1193
MPX: MPD5_0762
THL: TEH_06440(spxB)
OOE: OEOE_0936
LME: LEUM_0977
LMM: MI1_04655
LMK: LMES_0909
LKI: LKI_00650
LGS: LEGAS_1053(poxB)
WKO: WKK_00165
WCE: WS08_1253
WCT: WS74_1324
AUR: HMPREF9243_0738(spxB)
CRN: CAR_c09120(ydaP)
CARC: NY10_1620
JDA: BW727_101040(pox5)
FSA: C5Q98_07650(spxB)
SAY: TPY_2798(poxL)
MVQ: MYVA_3170(poxL)
MCHE: BB28_24185
MSTE: MSTE_04898
PFRE: RM25_0410
NDK: I601_3193(ydaP)
BAST: BAST_1463
SUS: Acid_7324
LFC: LFE_1301
METC: MTCT_0413
MWO: MWSIV6_0858(ydaP)
METE: tca_00442(ydaP)
METH: MBMB1_1304
MCUB: MCBB_0616(ydaP)
MMET: MCMEM_0420
MHU: Mhun_0630
MEMA: MMAB1_2008
MPL: Mpal_0137
TAA: NMY3_01752(ydaP_3)
 » show all
Taxonomy
Reference
1  [PMID:5336022]
  Authors
Williams FR, Hager LP.
  Title
Crystalline flavin pyruvate oxidase from Escherichia coli. I. Isolation and properties of the flavoprotein.
  Journal
Arch Biochem Biophys 116:168-76 (1966)
Reference
2  [PMID:16201755]
  Authors
Tittmann K, Wille G, Golbik R, Weidner A, Ghisla S, Hubner G.
  Title
Radical phosphate transfer mechanism for the thiamin diphosphate- and FAD-dependent pyruvate oxidase from Lactobacillus plantarum. Kinetic coupling of intercofactor electron transfer with phosphate transfer to acetyl-thiamin diphosphate via a transient FAD semiquinone/hydroxyethyl-ThDP radical pair.
  Journal
Biochemistry 44:13291-303 (2005)
DOI:10.1021/bi051058z
Other DBs
ExplorEnz - The Enzyme Database: 1.2.3.3
IUBMB Enzyme Nomenclature: 1.2.3.3
ExPASy - ENZYME nomenclature database: 1.2.3.3
BRENDA, the Enzyme Database: 1.2.3.3
CAS: 9001-96-1

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