KEGG   ENZYME: 1.2.7.7Help
Entry
EC 1.2.7.7                  Enzyme                                 

Name
3-methyl-2-oxobutanoate dehydrogenase (ferredoxin);
2-ketoisovalerate ferredoxin reductase;
3-methyl-2-oxobutanoate synthase (ferredoxin);
VOR;
branched-chain ketoacid ferredoxin reductase;
branched-chain oxo acid ferredoxin reductase;
keto-valine-ferredoxin oxidoreductase;
ketoisovalerate ferredoxin reductase;
2-oxoisovalerate ferredoxin reductase
Class
Oxidoreductases;
Acting on the aldehyde or oxo group of donors;
With an iron-sulfur protein as acceptor
BRITE hierarchy
Sysname
3-methyl-2-oxobutanoate:ferredoxin oxidoreductase (decarboxylating; CoA-2-methylpropanoylating)
Reaction(IUBMB)
3-methyl-2-oxobutanoate + CoA + 2 oxidized ferredoxin = S-(2-methylpropanoyl)-CoA + CO2 + 2 reduced ferredoxin + H+ [RN:R07160]
Reaction(KEGG)
R07160;
(other) R08566 R08567
Show
Substrate
3-methyl-2-oxobutanoate [CPD:C00141];
CoA [CPD:C00010];
oxidized ferredoxin [CPD:C00139]
Product
S-(2-methylpropanoyl)-CoA;
CO2 [CPD:C00011];
reduced ferredoxin [CPD:C00138];
H+ [CPD:C00080]
Comment
The enzyme is CoA-dependent and contains thiamine diphosphate and iron-sulfur clusters. Preferentially utilizes 2-oxo-acid derivatives of branched chain amino acids, e.g. 3-methyl-2-oxopentanoate, 4-methyl-2-oxo-pentanoate, and 2-oxobutanoate. This enzyme is a member of the 2-oxoacid oxidoreductases, a family of enzymes that reversibly catalyse the oxidative decarboxylation of different 2-oxoacids to form their CoA derivatives, and are differentiated based on their substrate specificity. For examples of other members of this family, see EC 1.2.7.1, pyruvate synthase, and EC 1.2.7.3, 2-oxoglutarate synthase.
History
EC 1.2.7.7 created 2003
Pathway
ec00280  Valine, leucine and isoleucine degradation
ec01100  Metabolic pathways
Orthology
K00186  2-oxoisovalerate ferredoxin oxidoreductase, alpha subunit
K00187  2-oxoisovalerate ferredoxin oxidoreductase, beta subunit
K00188  2-oxoisovalerate ferredoxin oxidoreductase, delta subunit
K00189  2-oxoisovalerate ferredoxin oxidoreductase, gamma subunit
Genes
PEF: A7E78_02850
DEJ: AWY79_02095
DML: Dmul_18540(porB)
AFW: Anae109_4113 Anae109_4114
DAV: DESACE_01535
PBU: L21SP3_01136 L21SP3_01138(korB_1)
PBP: STSP1_01191 STSP1_01193(korB_1)
PBAS: SMSP2_01215(korB) SMSP2_01217
VBC: C5Q97_08000 C5Q97_08010
SLR: L21SP2_0406 L21SP2_0407
CABY: Cabys_3127
MMP: MMP1271(vorA) MMP1272(vorB) MMP1273(vorC)
MMG: MTBMA_c10900(vorC) MTBMA_c10910(vorB) MTBMA_c10930(vorA2)
MWO: MWSIV6_1079(vorC) MWSIV6_1081(vorA)
METE: tca_00678 tca_00680(korB_2)
MRU: mru_1431(vorB) mru_1432(vorA) mru_1433(vorC)
MMIL: sm9_0692(vorB) sm9_0694(vorC)
MEYE: TL18_03385
METH: MBMB1_0178 MBMB1_0179(vorB) MBMB1_0180(vorA)
MFC: BRM9_2123(vorB) BRM9_2125(vorC)
MCUB: MCBB_0193(vorC) MCBB_0195(vorA)
TVO: TVG0871982(TVG0871982) TVG0872869(TVG0872869)
MEAR: Mpt1_c05540(vorA)
PHO: PH0678(PH0678) PH0679(PH0679) PH0680(PH0680) PH0681(PH0681)
PFI: PFC_04070 PFC_04075(vorA) PFC_04080(vorD)
TGA: TGAM_0264(vorB) TGAM_0265(vorA) TGAM_0266(vorD)
THE: GQS_08125 GQS_08130(vorA) GQS_08135(vorD)
TLT: OCC_07486 OCC_07491(porA) OCC_07496(vorD)
TEU: TEU_01670 TEU_01680(vorD)
PPAC: PAP_04305(vorD) PAP_04315
MAC: MA_2909(vorA) MA_2910(vorB) MA_2911(vorC)
MMA: MM_3181(vorC) MM_3182(vorB) MM_3183(vorA)
MCJ: MCON_0778(vorA) MCON_0779(vorB)
KCR: Kcr_1292
 » show all
Taxonomy
Reference
1  [PMID:5800441]
  Authors
Buchanan BB.
  Title
Role of ferredoxin in the synthesis of alpha-ketobutyrate from propionyl coenzyme A and carbon dioxide by enzymes from photosynthetic and nonphotosynthetic bacteria.
  Journal
J Biol Chem 244:4218-23 (1969)
Reference
2  [PMID:8550513]
  Authors
Heider J, Mai X, Adams MW.
  Title
Characterization of 2-ketoisovalerate ferredoxin oxidoreductase, a new and reversible coenzyme A-dependent enzyme involved in peptide fermentation by hyperthermophilic archaea.
  Journal
J Bacteriol 178:780-7 (1996)
DOI:10.1128/JB.178.3.780-787.1996
Reference
3  [PMID:9108258]
  Authors
Tersteegen A, Linder D, Thauer RK, Hedderich R.
  Title
Structures and functions of four anabolic 2-oxoacid oxidoreductases in Methanobacterium thermoautotrophicum.
  Journal
Eur J Biochem 244:862-8 (1997)
DOI:10.1111/j.1432-1033.1997.00862.x
Reference
4  [PMID:11265457]
  Authors
Schut GJ, Menon AL, Adams MW.
  Title
2-keto acid oxidoreductases from Pyrococcus furiosus and Thermococcus litoralis.
  Journal
Methods Enzymol 331:144-58 (2001)
DOI:10.1016/S0076-6879(01)31053-4
Other DBs
ExplorEnz - The Enzyme Database: 1.2.7.7
IUBMB Enzyme Nomenclature: 1.2.7.7
ExPASy - ENZYME nomenclature database: 1.2.7.7
BRENDA, the Enzyme Database: 1.2.7.7

DBGET integrated database retrieval system