KEGG   ENZYME: 1.2.7.7
Entry
EC 1.2.7.7                  Enzyme                                 
Name
3-methyl-2-oxobutanoate dehydrogenase (ferredoxin);
2-ketoisovalerate ferredoxin reductase;
3-methyl-2-oxobutanoate synthase (ferredoxin);
VOR;
branched-chain ketoacid ferredoxin reductase;
branched-chain oxo acid ferredoxin reductase;
keto-valine-ferredoxin oxidoreductase;
ketoisovalerate ferredoxin reductase;
2-oxoisovalerate ferredoxin reductase
Class
Oxidoreductases;
Acting on the aldehyde or oxo group of donors;
With an iron-sulfur protein as acceptor
Sysname
3-methyl-2-oxobutanoate:ferredoxin oxidoreductase (decarboxylating; CoA-2-methylpropanoylating)
Reaction(IUBMB)
3-methyl-2-oxobutanoate + CoA + 2 oxidized ferredoxin = S-(2-methylpropanoyl)-CoA + CO2 + 2 reduced ferredoxin + H+ [RN:R07160]
Reaction(KEGG)
R07160;
(other) R08566 R08567
Substrate
3-methyl-2-oxobutanoate [CPD:C00141];
CoA [CPD:C00010];
oxidized ferredoxin [CPD:C00139]
Product
S-(2-methylpropanoyl)-CoA;
CO2 [CPD:C00011];
reduced ferredoxin [CPD:C00138];
H+ [CPD:C00080]
Comment
The enzyme is CoA-dependent and contains thiamine diphosphate and iron-sulfur clusters. Preferentially utilizes 2-oxo-acid derivatives of branched chain amino acids, e.g. 3-methyl-2-oxopentanoate, 4-methyl-2-oxo-pentanoate, and 2-oxobutanoate. This enzyme is a member of the 2-oxoacid oxidoreductases, a family of enzymes that reversibly catalyse the oxidative decarboxylation of different 2-oxoacids to form their CoA derivatives, and are differentiated based on their substrate specificity. For examples of other members of this family, see EC 1.2.7.1, pyruvate synthase, and EC 1.2.7.3, 2-oxoglutarate synthase.
History
EC 1.2.7.7 created 2003
Pathway
ec00280  Valine, leucine and isoleucine degradation
ec01100  Metabolic pathways
Orthology
K00186  2-oxoisovalerate ferredoxin oxidoreductase alpha subunit
K00187  2-oxoisovalerate ferredoxin oxidoreductase beta subunit
K00188  2-oxoisovalerate ferredoxin oxidoreductase delta subunit
K00189  2-oxoisovalerate/pyruvate ferredoxin oxidoreductase gamma subunit
Genes
PEFA7E78_02850 A7E78_02855
DBKDGMP_23530
DALKDSCA_29970
DLIdnl_19240(porB)
DMMdnm_023500
DEKDSLASN_10380 DSLASN_10400(porA_1)
AFWAnae109_4113 Anae109_4114
HMRHipma_0796 Hipma_0797 Hipma_0798
DAVDESACE_01535 DESACE_01540(porA) DESACE_01545
DHRLGS26_04510
PFAAMM59RIKEN_15740
CALKHUE98_02340 HUE98_02350(porA)
FWADCMF_13980
MPAGC0J29_14490
PBUL21SP3_01136 L21SP3_01137(korA_1) L21SP3_01138(korB_1)
PBPSTSP1_01191 STSP1_01192(korA_1) STSP1_01193(korB_1)
PBASSMSP2_01215(korB) SMSP2_01216(korA) SMSP2_01217
VBCC5Q97_08000 C5Q97_08005 C5Q97_08010
SLRL21SP2_0405 L21SP2_0406 L21SP2_0407
ABAAcid345_0952 Acid345_0953
SUSAcid_1870 Acid_1871
PFERIRI77_33000 IRI77_33005
THYDTTHT_1225(porA) TTHT_1226(porB)
CABYCabys_3126 Cabys_3127
MMPMMP1271(vorA) MMP1272(vorB) MMP1273(vorC)
MMQMmarC5_0318 MmarC5_0319 MmarC5_0320
MMXMmarC6_1400 MmarC6_1401 MmarC6_1402
MMZMmarC7_0517 MmarC7_0518 MmarC7_0519
MMDGYY_07190 GYY_07195 GYY_07200
MMAKMMKA1_14960 MMKA1_14970 MMKA1_14980
MMAOMMOS7_13780 MMOS7_13790 MMOS7_13800
MMADMMJJ_15720 MMJJ_15730(korA_3) MMJJ_15740(korB_3)
MVNMevan_0583 Mevan_0584 Mevan_0585
MVOMvol_0718 Mvol_0719 Mvol_0720
MTHMTH_703 MTH_704 MTH_705
MMGMTBMA_c10900(vorC) MTBMA_c10910(vorB) MTBMA_c10920(vorA1) MTBMA_c10930(vorA2)
METCMTCT_0631 MTCT_0632 MTCT_0633
MWOMWSIV6_1079(vorC) MWSIV6_1080(vorB) MWSIV6_1081(vorA)
METEtca_00678 tca_00679(korA_2) tca_00680(korB_2)
METZMETMT2_0337 METMT2_0338 METMT2_0339
METKFVF72_08025 FVF72_08030 FVF72_08035
MTHMFZP57_05120 FZP57_05125 FZP57_05130
METJFZP68_01095 FZP68_01100 FZP68_01105
MTEEMTTB_10540 MTTB_10550 MTTB_10560
MRUmru_1431(vorB) mru_1432(vorA) mru_1433(vorC)
MSIMsm_0331 Msm_0332 Msm_0333
MMILsm9_0692(vorB) sm9_0693(vorA) sm9_0694(vorC)
MEYETL18_03385
MOLYLM1_0932 YLM1_0933 YLM1_0934
MAROMarbSA_03660 MarbSA_03670 MarbSA_03680
MELMetbo_2452 Metbo_2453 Metbo_2454
MEWMSWAN_0187 MSWAN_0188 MSWAN_0189
METHMBMB1_0178 MBMB1_0179(vorB) MBMB1_0180(vorA)
MFCBRM9_2123(vorB) BRM9_2124(vorA) BRM9_2125(vorC)
MFIDSM1535_1555 DSM1535_1556(vorB) DSM1535_1557(vorA)
MCUBMCBB_0193(vorC) MCBB_0194(vorB) MCBB_0195(vorA)
MSUBBK009_10645 BK009_10650 BK009_10655
METNBK008_06270 BK008_06275 BK008_06280
METTCIT01_01270 CIT01_01275 CIT01_01280
METOCIT02_06140 CIT02_06145 CIT02_06150
MEMEHYG87_02760 HYG87_02765 HYG87_02770
MFVMfer_0416 Mfer_0417 Mfer_0506
ASTAsulf_00880
GACGACE_2154
GAHGAH_00571
PFUPF0968 PF0969 PF0970 PF0971
PFIPFC_04070 PFC_04075(vorA) PFC_04080(vorD) PFC_04085
PHOPH0678(PH0678) PH0679(PH0679) PH0680(PH0680) PH0681(PH0681)
PABPAB1470 PAB1471 PAB1472 PAB1473
PYNPNA2_1292 PNA2_1293 PNA2_1294 PNA2_1295
PYAPYCH_06630 PYCH_06640 PYCH_06650 PYCH_06660
PYSPy04_1079 Py04_1080 Py04_1081 Py04_1082
PYCTQ32_04845 TQ32_04850(vorD) TQ32_04855(porA) TQ32_04860
TKOTK1978 TK1979 TK1980 TK1981
TONTON_1476 TON_1477 TON_1478 TON_1479
TGATGAM_0264(vorB) TGAM_0265(vorA) TGAM_0266(vorD) TGAM_0267(porG)
TSITSIB_1381 TSIB_1382 TSIB_1383 TSIB_1384
TBATERMP_01557 TERMP_01558 TERMP_01559 TERMP_01560
THEGQS_08125 GQS_08130(vorA) GQS_08135(vorD) GQS_08140
THATAM4_1107 TAM4_1398 TAM4_1406 TAM4_1460
THMCL1_1246 CL1_1247 CL1_1248 CL1_1249
TLTOCC_07486 OCC_07491(porA) OCC_07496(vorD) OCC_07501
THSTES1_1552 TES1_1553 TES1_1554 TES1_1555
TNUBD01_1941 BD01_1942 BD01_1943 BD01_1944
TEUTEU_01670 TEU_01675(porA) TEU_01680(vorD) TEU_01685
TGYX802_03760 X802_03765(porA) X802_03770(vorD) X802_03775
THVADU37_CDS14440 ADU37_CDS14450 ADU37_CDS14460 ADU37_CDS14470
TCHCHITON_0665 CHITON_0666 CHITON_0667 CHITON_0668
TPEPA0127_04995 A0127_05000(vorD) A0127_05005(porA) A0127_05010
TPIEA7C91_04895 A7C91_04900(porA) A7C91_04905(vorD) A7C91_04910
TGGA3K92_07955 A3K92_07960(vorD) A3K92_07965(porA) A3K92_07970
TCEA3L02_06800 A3L02_06805(porA) A3L02_06810(vorD) A3L02_06815
TBSA3L01_08180 A3L01_08185(vorD) A3L01_08190(porA) A3L01_08195
THHCDI07_07385 CDI07_07390(porA) CDI07_07395(vorD) CDI07_07400
TSLA3L11_01530 A3L11_01535(vorD) A3L11_01540(porA) A3L11_01545
TTDA3L14_10575 A3L14_10580(vorD) A3L14_10585(porA) A3L14_10590
TPRFA3L09_01305 A3L09_01310(vorD) A3L09_01315(porA) A3L09_01320
TRLA3L10_07570 A3L10_07575(vorD) A3L10_07580(porA) A3L10_07585
TPAFA3L08_01305 A3L08_01310(vorD) A3L08_01315(porA) A3L08_01320
THYA3L12_04015 A3L12_04020(vorD) A3L12_04025(porA) A3L12_04030
TICFH039_11775 FH039_11780 FH039_11785(porA) FH039_11790
TCQTIRI35C_1959(porG) TIRI35C_1960(vorD) TIRI35C_1961(vorA) TIRI35C_1962(vorB)
THEMFPV09_09745 FPV09_09750(porA) FPV09_09755 FPV09_09760
PPACPAP_04300 PAP_04305(vorD) PAP_04310(porA) PAP_04315
MBAMbar_A2173 Mbar_A2174 Mbar_A2175
MBYMSBRM_2262 MSBRM_2263 MSBRM_2264
MBWMSBRW_2566 MSBRW_2567 MSBRW_2568
MBARMSBR2_0147 MSBR2_0148 MSBR2_0149
MBAKMSBR3_1064 MSBR3_1065 MSBR3_1066
MACMA_2909(vorA) MA_2910(vorB) MA_2911(vorC)
MMAMM_3181(vorC) MM_3182(vorB) MM_3183(vorA)
MMAZMmTuc01_3277 MmTuc01_3278 MmTuc01_3279
MMJMSMAS_1506 MSMAS_1507 MSMAS_1508
MMACMSMAC_2849 MSMAC_2850 MSMAC_2851
MVCMSVAZ_1286 MSVAZ_1287 MSVAZ_1288
MEKMSKOL_1230 MSKOL_1231 MSKOL_1232
MLSMSLAZ_2008 MSLAZ_2009 MSLAZ_2010
METMMSMTP_1761 MSMTP_1762 MSMTP_1763
MEFMSWH1_1307 MSWH1_1308 MSWH1_1309
MEQMSWHS_1391 MSWHS_1392 MSWHS_1393
MSJMSSAC_2721 MSSAC_2722 MSSAC_2723
MSZMSSIH_1391 MSSIH_1392 MSSIH_1393
MSWMSSIT_1429 MSSIT_1430 MSSIT_1431
MTHRMSTHT_0824 MSTHT_0825 MSTHT_0826
MTHEMSTHC_2445 MSTHC_2446 MSTHC_2447
MHORMSHOH_1579 MSHOH_1580 MSHOH_1581
MFZAOB57_012375 AOB57_012380(vorB) AOB57_012385
MBUMbur_0605 Mbur_0606 Mbur_0607
MMETMCMEM_1077 MCMEM_1078 MCMEM_1079
MELOJ7W08_03845 J7W08_03850 J7W08_03855
MMHMmah_1010 Mmah_1011 Mmah_1012
MHAZBHR79_08510 BHR79_08515 BHR79_08520
MZHMzhil_0902 Mzhil_0903 Mzhil_0904
MPYMpsy_0059 Mpsy_0060 Mpsy_0061
MZIHWN40_07260 HWN40_07265 HWN40_07270
MHZMetho_2048 Metho_2049 Metho_2050
MTPMthe_0156 Mthe_0157
MCJMCON_0778(vorA) MCON_0779(vorB)
MHIMhar_0583 Mhar_0584
TACTa0626 Ta0627 Ta0628 Ta0629
TVOTVG0872869(TVG0872869)
MERMMINT_12450(vorD) MMINT_12470
MEARMpt1_c05540(vorA) Mpt1_c05550(vorB)
ABIAboo_0298 Aboo_0299 Aboo_0300 Aboo_0301
ACFAciM339_0763 AciM339_0764 AciM339_0765 AciM339_0766
SMRSmar_0998
SHCShell_1463
DKADKAM_1187
DFDDesfe_1298
DMUDesmu_1191
TAGTagg_0827
IAGIgag_1136
THGTCELL_0309
HBUHbut_0730
PDLPyrde_1448
SOHD1869_08595
SOLSsol_2171
SSOASULA_2206
SSOLSULB_2207
SSOFSULC_2204
SSHIJ5U23_01993
SISLS215_0396
SIAM1425_0372
SIMM1627_0380
SIDM164_0396
SIYYG5714_0374
SINYN1551_2681
SIILD85_0384
SIHSiH_0373
SIRSiRe_0364
SICSiL_0352
SULABFU36_06385
SULEGFS03_03425
SULOGFS33_07805
MTENGWK48_07885
ASCASAC_1054
ACIASE86_06405
CLGCalag_0568
FFOFFONT_0327
CCAINAS2_0099 NAS2_0791
FLTSv326_0312
PSYTDSAG12_00253(vorA_1) DSAG12_00810(porG_2)
AGWQT03_C0001G0327
 » show all
Reference
1  [PMID:5800441]
  Authors
Buchanan BB.
  Title
Role of ferredoxin in the synthesis of alpha-ketobutyrate from propionyl coenzyme A and carbon dioxide by enzymes from photosynthetic and nonphotosynthetic bacteria.
  Journal
J Biol Chem 244:4218-23 (1969)
Reference
2  [PMID:8550513]
  Authors
Heider J, Mai X, Adams MW
  Title
Characterization of 2-ketoisovalerate ferredoxin oxidoreductase, a new and reversible coenzyme A-dependent enzyme involved in peptide fermentation by hyperthermophilic archaea.
  Journal
J Bacteriol 178:780-7 (1996)
DOI:10.1128/JB.178.3.780-787.1996
Reference
3  [PMID:9108258]
  Authors
Tersteegen A, Linder D, Thauer RK, Hedderich R
  Title
Structures and functions of four anabolic 2-oxoacid oxidoreductases in Methanobacterium thermoautotrophicum.
  Journal
Eur J Biochem 244:862-8 (1997)
DOI:10.1111/j.1432-1033.1997.00862.x
Reference
4  [PMID:11265457]
  Authors
Schut GJ, Menon AL, Adams MW.
  Title
2-keto acid oxidoreductases from Pyrococcus furiosus and Thermococcus litoralis.
  Journal
Methods Enzymol 331:144-58 (2001)
DOI:10.1016/S0076-6879(01)31053-4
Other DBs
ExplorEnz - The Enzyme Database: 1.2.7.7
IUBMB Enzyme Nomenclature: 1.2.7.7
ExPASy - ENZYME nomenclature database: 1.2.7.7
BRENDA, the Enzyme Database: 1.2.7.7

DBGET integrated database retrieval system