KEGG   ENZYME: 1.21.3.2Help
Entry
EC 1.21.3.2                 Enzyme                                 

Name
columbamine oxidase;
berberine synthase
Class
Oxidoreductases;
Catalysing the reaction X-H + Y-H = X-Y;
With oxygen as acceptor
BRITE hierarchy
Sysname
columbamine:oxygen oxidoreductase (cyclizing)
Reaction(IUBMB)
2 columbamine + O2 = 2 berberine + 2 H2O [RN:R00044]
Reaction(KEGG)
Substrate
columbamine [CPD:C01795];
O2 [CPD:C00007]
Product
berberine [CPD:C00757];
H2O [CPD:C00001]
Comment
An iron protein. Oxidation of the O-methoxyphenol structure forms the methylenedioxy group of berberine.
History
EC 1.21.3.2 created 1989 as EC 1.1.3.26, transferred 2002 to EC 1.21.3.2
Pathway
ec00950  Isoquinoline alkaloid biosynthesis
ec01110  Biosynthesis of secondary metabolites
Reference
1
  Authors
Rueffer, M. and Zenk, M.H.
  Title
Berberine synthesis, the methylenedioxy group forming enzyme in berberine synthesis.
  Journal
Tetrahedron Lett 26:201-202 (1985)
Other DBs
ExplorEnz - The Enzyme Database: 1.21.3.2
IUBMB Enzyme Nomenclature: 1.21.3.2
ExPASy - ENZYME nomenclature database: 1.21.3.2
BRENDA, the Enzyme Database: 1.21.3.2
CAS: 95329-18-3

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