KEGG   ENZYME: 1.3.1.1Help
Entry
EC 1.3.1.1                  Enzyme                                 

Name
dihydropyrimidine dehydrogenase (NAD+);
dihydropyrimidine dehydrogenase;
dihydrothymine dehydrogenase;
pyrimidine reductase;
thymine reductase;
uracil reductase;
dihydrouracil dehydrogenase (NAD+)
Class
Oxidoreductases;
Acting on the CH-CH group of donors;
With NAD+ or NADP+ as acceptor
BRITE hierarchy
Sysname
5,6-dihydropyrimidine:NAD+ oxidoreductase
Reaction(IUBMB)
(1) 5,6-dihydrouracil + NAD+ = uracil + NADH + H+ [RN:R00977];
(2) 5,6-dihydrothymine + NAD+ = thymine + NADH + H+ [RN:R01414]
Reaction(KEGG)
Substrate
5,6-dihydrouracil [CPD:C00429];
NAD+ [CPD:C00003];
5,6-dihydrothymine [CPD:C00906]
Product
uracil [CPD:C00106];
NADH [CPD:C00004];
H+ [CPD:C00080];
thymine [CPD:C00178]
Comment
An iron-sulfur flavoenzyme. The enzyme was originally discovered in the uracil-fermenting bacterium, Clostridium uracilicum, which utilizes uracil and thymine as nitrogen and carbon sources for growth [1]. Since then the enzyme was found in additional organisms including Alcaligenes eutrophus [2], Pseudomonas strains [3,4] and Escherichia coli [5,6].
History
EC 1.3.1.1 created 1961, modified 2011
Pathway
ec00240  Pyrimidine metabolism
ec00410  beta-Alanine metabolism
ec00770  Pantothenate and CoA biosynthesis
ec01100  Metabolic pathways
Orthology
K17722  dihydropyrimidine dehydrogenase (NAD+) subunit PreT
K17723  dihydropyrimidine dehydrogenase (NAD+) subunit PreA
Genes
ECO: b2146(preT) b2147(preA)
ECJ: JW2133(yeiT) JW2134(yeiA)
ECD: ECDH10B_2303(yeiT) ECDH10B_2304(yeiA)
EBW: BWG_1928(yeiT) BWG_1929(yeiA)
ECOK: ECMDS42_1715(yeiT) ECMDS42_1716(yeiA)
ECE: Z3401 Z3402(yeiA)
ECS: ECs3038 ECs3039
ECF: ECH74115_3278 ECH74115_3279
ETW: ECSP_3024(yeiT) ECSP_3025(yeiA)
ELX: CDCO157_2801 CDCO157_2802
EOJ: ECO26_3058(yeiT) ECO26_3059(yeiA)
EOI: ECO111_2863(yeiT) ECO111_2864(yeiA)
EOH: ECO103_2621(yeiT) ECO103_2622(yeiA)
ECG: E2348C_2292(yeiT) E2348C_2293(yeiA)
EOK: G2583_2689(yeiT) G2583_2690(yeiA)
ECC: c2679(yeiT) c2680(yeiA)
ECI: UTI89_C2419(yeiT) UTI89_C2420(yeiA)
ECV: APECO1_4404(yeiA) APECO1_4405(yeiT)
ECR: ECIAI1_2223(yeiT) ECIAI1_2224(yeiA)
ECQ: ECED1_2593(yeiT) ECED1_2594(yeiA)
ECK: EC55989_2396(yeiT) EC55989_2397(yeiA)
ECT: ECIAI39_2285(yeiT) ECIAI39_2286(yeiA)
EOC: CE10_2517(yeiT) CE10_2518(yeiA)
EUM: ECUMN_2479(yeiT) ECUMN_2480(yeiA)
ECZ: ECS88_2292(yeiT) ECS88_2293(yeiA)
EBR: ECB_02075(yeiT) ECB_02076(yeiA)
EKF: KO11_11895(yeiA) KO11_11900(yeiT)
EAB: ECABU_c24760(yeiT) ECABU_c24770(yeiA)
ELW: ECW_m2347(yeiT) ECW_m2348(yeiA)
ELL: WFL_11480(yeiT) WFL_11485(yeiA)
ELC: i14_2478(yeiT) i14_2479(yeiA)
ELD: i02_2478(yeiT) i02_2479(yeiA)
EBL: ECD_02075(preT) ECD_02076(preA)
EBE: B21_02034(yeiT) B21_02035(yeiA)
ELF: LF82_3007(yeiA) LF82_3021(yeiT)
ECOI: ECOPMV1_02307(preT) ECOPMV1_02308(preA)
ECOS: EC958_2483(yeiT) EC958_2484(yeiA)
EFE: EFER_2231(yeiT) EFER_2232(yeiA)
STY: STY2416
STT: t0669
STM: STM2186 STM2187(yeiA)
SPT: SPA0664(yeiA) SPA0665
SEI: SPC_1515(yeiA) SPC_1516
SEC: SCH_2203(yeiT) SCH_2204(yeiA)
SEG: SG2222 SG2223(yeiA)
SEL: SPUL_0702(yeiA) SPUL_0703
SET: SEN2179 SEN2180(yeiA)
SFL: SF2231
SFX: S2360
SFV: SFV_2221
SFE: SFxv_2463(yeiT)
SFT: NCTC1_02451(preT) NCTC1_02452(preA_1) NCTC1_02453(preA_2)
SSN: SSON_2203(yeiA)
SBO: SBO_0995
SDY: SDY_2140a
PAU: PA14_05740(pydA) PA14_05750(gltX)
PNC: NCGM2_5757(gltX) NCGM2_5758(pydA)
PAEB: NCGM1900_0449(pydA) NCGM1900_0450(gltX)
PPSE: BN5_0328(pydA) BN5_0329(gltD1)
PPU: PP_4037(pydX) PP_4038(pydA)
PPX: T1E_3293(pyd1) T1E_3294(yeiA)
PPUN: PP4_17170(preA) PP4_17180(preT)
PPRO: PPC_2590(pyrD) PPC_2591
PPUU: PputUW4_02929(gltD2) PputUW4_02930(pyrD1)
PSOS: POS17_2548(pyrD) POS17_2549
GAI: IMCC3135_08095(preT) IMCC3135_08100(preA)
BMJ: BMULJ_06147(pyrD) BMULJ_06148(gltD)
OFO: BRW83_1759(preT) BRW83_1760(preA)
THI: THI_3627(pydA) THI_3628
GLO: Glov_2025
SUR: STAUR_8045(gltB) STAUR_8046(yeiA)
SCL: sce6397(yeiA) sce6398(gltB2)
AMIH: CO731_02730(preT) CO731_02731(preA)
RHI: NGR_c24760(gltB2) NGR_c24770
SFD: USDA257_c49080(preT) USDA257_c49090(preA)
EAD: OV14_4003(gltB2) OV14_4004
REL: REMIM1_CH03338(preT) REMIM1_CH03339(preA)
REP: IE4803_CH03512(preT) IE4803_CH03513(preA)
REI: IE4771_CH03549(preT) IE4771_CH03550(preA)
RLE: RL3707 RL3708(pydA)
RHL: LPU83_3156(gltB1) LPU83_3157
RGA: RGR602_CH02885(preT) RGR602_CH02886(preA)
RHN: AMJ98_CH03475(preT) AMJ98_CH03476(preA)
RPHA: AMC79_CH03444(preT) AMC79_CH03445(preA)
RHT: NT26_2519 NT26_2520(pydA)
RHX: AMK02_CH03364(preT) AMK02_CH03365(preA)
RHK: Kim5_CH03553(preT) Kim5_CH03554(preA)
MMED: Mame_01300(preA) Mame_01301(preT)
HDI: HDIA_1458(gltD) HDIA_1459(preA)
DSH: Dshi_1362 Dshi_1363(pyrD1)
OTM: OSB_04390(preA) OSB_04410(gltD_1)
KEU: S101446_01662(preA) S101446_01663(gltD)
ASZ: ASN_1388(gltD) ASN_1389
AZL: AZL_d01290(dpyD) AZL_d01300(gltD)
TMO: TMO_c0377(yeiA) TMO_c0378(yeiT)
BCZ: pE33L466_0262(gltB)
BMH: BMWSH_0810(gltB)
LSP: Bsph_2011
HHD: HBHAL_2643(gltB2) HBHAL_2644
CBN: CbC4_1672 CbC4_1673(gltA)
CBM: CBF_2860
CBE: Cbei_1943
CBZ: Cbs_1943
CBEI: LF65_02110
CKL: CKL_2399(pyrD1)
CKR: CKR_2115
CSR: Cspa_c41330(preA)
CLD: CLSPO_c28940(preA) CLSPO_c28950(preT)
ESR: ES1_26140
ESU: EUS_26160
RCH: RUM_00500
CPRO: CPRO_21510(preA) CPRO_21520(preT)
SGY: Sgly_1379
SAY: TPY_1319(gltD) TPY_1320
TPZ: Tph_c23940(pyrD2) Tph_c24020(pyrD3)
DGO: DGo_CA0639(yeiA) DGo_CA0640(gltB)
MIN: Minf_0626(pyrD) Minf_0627(gltD)
ABAC: LuPra_00912(preA_1)
PDI: BDI_1170
CEX: CSE_02230
STO: STK_11200(pydA)
BARC: AOA65_0974(hdlA_1)
 » show all
Taxonomy
Reference
1  [PMID:13462991]
  Authors
CAMPBELL LL Jr.
  Title
Reductive degradation of pyrimidines. III. Purification and properties of dihydrouracil dehydrogenase.
  Journal
J Biol Chem 227:693-700 (1957)
Reference
2  [PMID:8806723]
  Authors
Schmitt U, Jahnke K, Rosenbaum K, Cook PF, Schnackerz KD
  Title
Purification and characterization of dihydropyrimidine dehydrogenase from Alcaligenes eutrophus.
  Journal
Arch Biochem Biophys 332:175-82 (1996)
DOI:10.1006/abbi.1996.0330
Reference
3  [PMID:1903745]
  Authors
Kim S, West TP
  Title
Pyrimidine catabolism in Pseudomonas aeruginosa.
  Journal
FEMS Microbiol Lett 61:175-9 (1991)
Reference
4  [PMID:11759049]
  Authors
West TP
  Title
Pyrimidine base catabolism in Pseudomonas putida biotype B.
  Journal
Antonie Van Leeuwenhoek 80:163-7 (2001)
Reference
5  [PMID:10030006]
  Authors
West TP
  Title
Isolation and characterization of an Escherichia coli B mutant strain defective in uracil catabolism.
  Journal
Can J Microbiol 44:1106-9 (1998)
Reference
6  [PMID:21169495]
  Authors
Hidese R, Mihara H, Kurihara T, Esaki N
  Title
Escherichia coli dihydropyrimidine dehydrogenase is a novel NAD-dependent heterotetramer essential for the production of 5,6-dihydrouracil.
  Journal
J Bacteriol 193:989-93 (2011)
DOI:10.1128/JB.01178-10
  Sequence
[eco:b2146 b2147]
Other DBs
ExplorEnz - The Enzyme Database: 1.3.1.1
IUBMB Enzyme Nomenclature: 1.3.1.1
ExPASy - ENZYME nomenclature database: 1.3.1.1
BRENDA, the Enzyme Database: 1.3.1.1
CAS: 9026-89-5

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