KEGG   ENZYME: 1.3.1.22Help
Entry
EC 1.3.1.22                 Enzyme                                 

Name
3-oxo-5alpha-steroid 4-dehydrogenase (NADP+);
cholestenone 5alpha-reductase;
testosterone Delta4-5alpha-reductase;
steroid 5alpha-reductase;
3-oxosteroid Delta4-dehydrogenase;
5alpha-reductase;
steroid 5alpha-hydrogenase;
3-oxosteroid 5alpha-reductase;
testosterone Delta4-hydrogenase;
4-ene-3-oxosteroid 5alpha-reductase;
reduced nicotinamide adenine dinucleotide phosphate:Delta4-3-ketosteroid 5alpha-oxidoreductase;
4-ene-5alpha-reductase;
Delta4-3-ketosteroid 5alpha-oxidoreductase;
cholest-4-en-3-one 5alpha-reductase;
testosterone 5alpha-reductase;
3-oxo-5alpha-steroid 4-dehydrogenase
Class
Oxidoreductases;
Acting on the CH-CH group of donors;
With NAD+ or NADP+ as acceptor
BRITE hierarchy
Sysname
3-oxo-5alpha-steroid:NADP+ Delta4-oxidoreductase
Reaction(IUBMB)
a 3-oxo-5alpha-steroid + NADP+ = a 3-oxo-Delta4-steroid + NADPH + H+ [RN:R02610]
Reaction(KEGG)
Substrate
3-oxo-5alpha-steroid [CPD:C02940];
NADP+ [CPD:C00006]
Product
3-oxo-Delta4-steroid [CPD:C00619];
NADPH [CPD:C00005];
H+ [CPD:C00080]
Comment
The enzyme catalyses the conversion of assorted 3-oxo-Delta4 steroids into their corresponding 5alpha form. Substrates for the mammalian enzyme include testosterone, progesterone, and corticosterone. Substrates for the plant enzyme are brassinosteroids such as campest-4-en-3-one and (22alpha)-hydroxy-campest-4-en-3-one. cf. EC 1.3.99.5, 3-oxo-5alpha-steroid 4-dehydrogenase (acceptor).
History
EC 1.3.1.22 created 1972, modified 2012
Pathway
ec00140  Steroid hormone biosynthesis
ec00905  Brassinosteroid biosynthesis
ec01100  Metabolic pathways
ec01110  Biosynthesis of secondary metabolites
Orthology
K09591  steroid 5-alpha-reductase
K12343  3-oxo-5-alpha-steroid 4-dehydrogenase 1
K12344  3-oxo-5-alpha-steroid 4-dehydrogenase 2
K12345  3-oxo-5-alpha-steroid 4-dehydrogenase 3
Genes
HSA: 6715(SRD5A1) 6716(SRD5A2) 79644(SRD5A3)
PTR: 100614992(SRD5A2) 471241(SRD5A3) 743740(SRD5A1)
PPS: 100978730(SRD5A1) 100986960(SRD5A2) 100987485(SRD5A3)
GGO: 101127484(SRD5A3) 101128506 101138758(SRD5A2) 101143956(SRD5A1)
PON: 100444341(SRD5A1) 100454013(SRD5A2) 100461714(SRD5A3)
NLE: 100597634(SRD5A2) 100604898(SRD5A3) 100607932(SRD5A1)
MCC: 695103(SRD5A1) 696381(SRD5A3) 705676(SRD5A2)
MCF: 102115639(SRD5A2) 102126671(SRD5A1) 102135568(SRD5A3)
CSAB: 103214971(SRD5A1) 103220536(SRD5A2) 103235668(SRD5A3)
RRO: 104662017(SRD5A3) 104669588(SRD5A2) 104672270(SRD5A1) 104674089
RBB: 108516633(SRD5A2) 108522884(SRD5A1) 108531594(SRD5A3)
CJC: 100386366(SRD5A1) 100409457(SRD5A3) 100415292(SRD5A2)
SBQ: 101035698(SRD5A3) 101043426(SRD5A2) 101044295(SRD5A1)
MMU: 57357(Srd5a3) 78925(Srd5a1) 94224(Srd5a2)
RNO: 24950(Srd5a1) 305291(Srd5a3) 64677(Srd5a2)
CGE: 100761447(Srd5a1) 100766917(Srd5a2) 100770660(Srd5a3)
NGI: 103734389(Srd5a3) 103735348(Srd5a1) 103748375(Srd5a2)
HGL: 101708836(Srd5a2) 101714960(Srd5a1) 101726767(Srd5a3)
CCAN: 109693823(Srd5a1) 109698967(Srd5a3) 109702049(Srd5a2)
OCU: 100343882(SRD5A2) 100346041(SRD5A3) 100349876(SRD5A1)
TUP: 102478179(SRD5A2) 102493739(SRD5A3) 102500370(SRD5A1) 106736078 106736081
CFA: 403715(SRD5A2) 403716(SRD5A1) 482155(SRD5A3)
AML: 100463734(SRD5A3) 100466687(SRD5A2) 100472194(SRD5A1)
UMR: 103671605(SRD5A2) 103672741(SRD5A3) 103679225(SRD5A1)
ORO: 101363313(SRD5A1) 101370876(SRD5A3) 101376466(SRD5A2)
FCA: 101086898(SRD5A2) 101090010(SRD5A3) 101098848(SRD5A1)
PTG: 102951833(SRD5A1) 102953790(SRD5A2) 102960215(SRD5A3)
AJU: 106971261(SRD5A1)
BTA: 527024(SRD5A2) 535834(SRD5A3) 614612(SRD5A1)
BOM: 102271230(SRD5A2) 102273138(SRD5A1) 102284607(SRD5A3)
BIU: 109560479(SRD5A3) 109565724(SRD5A2) 109574873(SRD5A1)
PHD: 102326516(SRD5A2) 102328275(SRD5A1) 102331048(SRD5A3)
CHX: 102172667(SRD5A1) 102180581(SRD5A3) 102187232(SRD5A2)
OAS: 100642181(SRD5A1) 100820756(SRD5A2) 101106772(SRD5A3)
SSC: 100518046(SRD5A1) 100525350 397048(SRD5A2)
CFR: 102514136(SRD5A1) 102523042(SRD5A3) 102523831(SRD5A2)
CDK: 105091542(SRD5A3) 105094037(SRD5A1) 105095594(SRD5A2)
BACU: 102998118(SRD5A1) 103005044 103012155(SRD5A3)
LVE: 103070353(SRD5A1) 103084942(SRD5A2) 103087526(SRD5A3)
OOR: 101276021 101284817(SRD5A2) 101287651(SRD5A1) 101290031(SRD5A3)
ECB: 100059582(SRD5A3) 100070655(SRD5A2) 100071450(SRD5A1)
EPZ: 103550080(SRD5A1) 103554975(SRD5A3) 103561662(SRD5A2)
EAI: 106822987(SRD5A1) 106836922(SRD5A3) 106847216(SRD5A2)
MYB: 102242056(SRD5A2) 102257134(SRD5A3) 102260657(SRD5A1)
MYD: 102762730(SRD5A1) 102771502(SRD5A3) 102773080(SRD5A2)
HAI: 109376001(SRD5A1) 109381825(SRD5A3) 109388788(SRD5A2)
RSS: 109437897(SRD5A2) 109451723(SRD5A3) 109453581(SRD5A1)
PALE: 102884992(SRD5A2) 102889165(SRD5A1) 102896891(SRD5A3)
LAV: 100671020(SRD5A3) 100673173(SRD5A1)
MDO: 100017228(SRD5A3) 100018123(SRD5A1) 100031489(SRD5A2)
SHR: 100920954(SRD5A1) 100928836(SRD5A3) 100934924(SRD5A2)
OAA: 100075423(SRD5A3) 100078235(SRD5A2)
GGA: 422750(SRD5A3) 770453(SRD5A1) 772291(SRD5A2)
MGP: 100540890(SRD5A1) 100541554(SRD5A3) 100546745(SRD5A2)
CJO: 107309501(SRD5A1) 107311306(SRD5A2) 107313729(SRD5A3)
APLA: 101792279(SRD5A1) 101796282(SRD5A3) 101799397(SRD5A2)
ACYG: 106030826(SRD5A3) 106036530(SRD5A1) 106049776(SRD5A2)
TGU: 100224163(SRD5A3) 100225484(SRD5A2) 101233999(SRD5A1)
GFR: 102038097(SRD5A2) 102040059(SRD5A1) 102042544(SRD5A3)
FAB: 101808258(SRD5A2) 101812956(SRD5A3) 101814400(SRD5A1)
PHI: 102108406(SRD5A2) 102110867(SRD5A1) 102113098(SRD5A3)
PMAJ: 107200161(SRD5A1) 107201346(SRD5A2) 107202960(SRD5A3)
CCAE: 111925370(SRD5A1) 111926994(SRD5A2) 111928443(SRD5A3)
CCW: 104684391(SRD5A2) 104685924(SRD5A1) 104690628(SRD5A3)
FPG: 101911819(SRD5A1) 101919492(SRD5A2) 101920408(SRD5A3)
FCH: 102046391(SRD5A2) 102049514(SRD5A1) 102059273(SRD5A3)
CLV: 102085278(SRD5A2) 102086070(SRD5A1) 102095131(SRD5A3)
EGZ: 104124808(SRD5A2) 104126190(SRD5A1) 104129459(SRD5A3)
AAM: 106485465(SRD5A2) 106490085(SRD5A3) 106492345(SRD5A1)
ASN: 102379264 102380496(SRD5A1) 102380562(SRD5A2) 102384757
AMJ: 102565754(SRD5A1) 102567202(SRD5A3) 102573116(SRD5A2)
PSS: 102444263(SRD5A3) 102449134(SRD5A1) 102451701(SRD5A2)
CMY: 102936118(SRD5A2) 102936322(SRD5A3) 102940906(SRD5A1)
CPIC: 101931675(SRD5A1) 101937467(SRD5A3) 101939421
ACS: 100551938(srd5a3) 100555660(srd5a1) 100563846(srd5a2) 103282902
PVT: 110076132(SRD5A2) 110078425(SRD5A3) 110080024(SRD5A1)
PBI: 103048475(SRD5A2) 103063410(SRD5A3) 103065332(SRD5A1)
GJA: 107106033(SRD5A3) 107108075(SRD5A2) 107123746(SRD5A1)
XLA: 100049757(srd5a1.S) 108698470(srd5a3.L) 108716702(srd5a2.L) 108717848(srd5a2.S) 379123(srd5a3.S)
XTR: 448586(srd5a1) 549867(srd5a2) 779994(srd5a3)
NPR: 108789853(SRD5A2) 108795133(SRD5A1) 108804033(SRD5A3)
DRE: 550388(srd5a2b) 550398(srd5a2a) 560717(srd5a3) 767715(srd5a1)
IPU: 108258126(srd5a3) 108258997(srd5a2) 108272332(srd5a1)
AMEX: 103036227(srd5a2) 103038993(srd5a1) 103043083 103045498(srd5a3)
TRU: 101063216(srd5a1) 101066856(srd5a2) 105417901(srd5a3)
LCO: 104918211(srd5a2) 104919092(srd5a3) 104922765(srd5a1) 104939523
NCC: 104947078(srd5a3) 104959824
MZE: 101473345(srd5a3) 101475598(srd5a1) 101476244 101484346
OLA: 100125491(srd5a1) 100125532 101156806(srd5a3) 101156869
XMA: 102220105(srd5a1) 102228249 102230435(srd5a2) 102237479(srd5a3)
PRET: 103463835(srd5a3) 103471931 103472068(srd5a1) 103477241
NFU: 107375448(srd5a2) 107383183(srd5a1) 107393391(srd5a3) 107394001
KMR: 108229061 108238724(srd5a1) 108243377(srd5a3) 108248141(srd5a2)
CSEM: 103376703(srd5a3) 103383286 103394093(srd5a1)
LCF: 108879013(srd5a3) 108882308 108885816(srd5a2) 108900396(srd5a1)
SDU: 111220716(srd5a2) 111226375(srd5a3) 111233184(srd5a1) 111237591
HCQ: 109508284(srd5a1) 109516280(srd5a2) 109532109(srd5a3)
BPEC: 110153623 110154923(srd5a3) 110157251(srd5a2) 110167484(srd5a1)
MALB: 109962082(srd5a3) 109971730(srd5a1) 109971818(srd5a2)
SASA: 100196185(s5a2) 106564819(S5A2) 106584121(srd5a3) 106588696(srd5a1)
ELS: 105011840(srd5a3) 105012407(srd5a1) 105029457 105031435
LCM: 102346093(SRD5A1) 102355026(SRD5A2) 102363132(SRD5A3)
CMK: 103178169(srd5a3) 103181748(srd5a2) 103183194(srd5a1)
SKO: 100378021
DME: Dmel_CG7840(CG7840)
DSI: Dsimw501_GD23520(Dsim_GD23520)
AAG: 5575734
AME: 102656812
BIM: 100744185
BTER: 100647495
AEC: 105149417
ACEP: 105627262
CFO: 105257288
PCF: 106788533
NVI: 100119448
MDL: 103569697
NVL: 108560909
BMOR: 101735516
PRAP: 110999351
HAW: 110370980
PXY: 105381317
CLEC: 106666251
ZNE: 110834339
CEL: CELE_B0024.13(B0024.13) CELE_F19H6.4(F19H6.4) CELE_F42F12.3(F42F12.3)
BMY: Bm1_06570
SHX: MS3_06000
EGL: EGR_04368
HMG: 100197205
ATH: AT2G16530 AT2G38050(DET2)
LJA: Lj0g3v0256849.1(Lj0g3v0256849.1) Lj3g3v3396900.1(Lj3g3v3396900.1)
DOSA: Os01t0851600-01(Os01g0851600) Os04t0576800-01(Os04g0576800) Os07t0493400-01(Os07g0493400) Os11t0184100-01(Os11g0184100)
ATS: 109743537(LOC109743537) 109764908(LOC109764908)
MNG: MNEG_6120
SCE: YIL049W(DFG10)
ERC: Ecym_4334
KMX: KLMA_70185(DFG10)
NCS: NCAS_0A13460(NCAS0A13460)
NDI: NDAI_0A02580(NDAI0A02580)
TPF: TPHA_0K00940(TPHA0K00940)
TBL: TBLA_0J01440(TBLA0J01440)
TDL: TDEL_0H02090(TDEL0H02090)
KAF: KAFR_0I01850(KAFR0I01850)
PIC: PICST_31592(DFG10)
CAL: CAALFM_C208970CA(DFG10)
CDU: CD36_23150(DFG10)
CAUR: QG37_06040
NTE: NEUTE1DRAFT82283(NEUTE1DRAFT_82283)
ANI: AN4071.2
ANG: ANI_1_1622184(An04g01740) ANI_1_570164(An18g04290)
CNE: CNA03510
CNB: CNBA3370
ABP: AGABI1DRAFT59271(AGABI1DRAFT_59271)
ABV: AGABI2DRAFT224531(AGABI2DRAFT_224531)
MGL: MGL_3451
DFA: DFA_07066
EHI: EHI_197070(92.t00023)
PYO: PY17X_1323400(PY01579)
SMIN: v1.2.019044.t1(symbB.v1.2.019044.t1)
PTI: PHATRDRAFT_17044(DET2)
SPAR: SPRG_09830
LLA: L176238(yfhH)
MUL: MUL_3208
MMI: MMAR_2552
MAB: MAB_2819
NFA: NFA_45190
REQ: REQ_08000
ACTN: L083_3086
TDE: TDE2697
TPED: TPE_0965
BTH: BT_2068
BFR: BF3752
BVU: BVU_1964
BXY: BXY_12340
AFD: Alfi_2922
ASH: AL1_03180
FLM: MY04_0311
 » show all
Taxonomy
Reference
1  [PMID:13673006]
  Authors
LEVY HR, TALALAY P.
  Title
Bacterial oxidation of steroids. II. Studies on the enzymatic mechanism of ring A dehydrogenation.
  Journal
J Biol Chem 234:2014-21 (1959)
Reference
2  [PMID:5907469]
  Authors
Shefer S, Hauser S, Mosbach EH.
  Title
Studies on the biosynthesis of 5-alpha-cholestan-3-beta-ol. I. Cholestenone 5-alpha-reductase of rat liver.
  Journal
J Biol Chem 241:946-52 (1966)
Reference
3  [PMID:1166484]
  Authors
Cheng YJ, Karavolas HJ.
  Title
Properties and subcellular distribution of delta4-steroid (progesterone) 5alpha-reductase in rat anterior pituitary.
  Journal
Steroids 26:57-71 (1975)
DOI:10.1016/0039-128X(75)90006-9
Reference
4  [PMID:1705142]
  Authors
Sargent NS, Habib FK
  Title
Partial purification of human prostatic 5 alpha-reductase (3-oxo-5 alpha-steroid:NADP+ 4-ene-oxido-reductase; EC 1.3.1.22) in a stable and active form.
  Journal
J Steroid Biochem Mol Biol 38:73-7 (1991)
DOI:10.1016/0960-0760(91)90403-R
Reference
5  [PMID:7900170]
  Authors
Quemener E, Amet Y, di Stefano S, Fournier G, Floch HH, Abalain JH
  Title
Purification of testosterone 5 alpha-reductase from human prostate by a four-step chromatographic procedure.
  Journal
Steroids 59:712-8 (1994)
DOI:10.1016/0039-128X(94)90103-1
Reference
6  [PMID:9202401]
  Authors
Poletti A, Celotti F, Rumio C, Rabuffetti M, Martini L
  Title
Identification of type 1 5alpha-reductase in myelin membranes of male and female  rat brain.
  Journal
Mol Cell Endocrinol 129:181-90 (1997)
DOI:10.1016/S0303-7207(97)04056-2
Reference
7  [PMID:9108014]
  Authors
Li J, Biswas MG, Chao A, Russell DW, Chory J
  Title
Conservation of function between mammalian and plant steroid 5alpha-reductases.
  Journal
Proc Natl Acad Sci U S A 94:3554-9 (1997)
DOI:10.1073/pnas.94.8.3554
  Sequence
[ath:AT2G38050]
Reference
8  [PMID:15993049]
  Authors
Rosati F, Bardazzi I, De Blasi P, Simi L, Scarpi D, Guarna A, Serio M, Racchi ML, Danza G.
  Title
5alpha-Reductase activity in Lycopersicon esculentum: cloning and functional characterization of LeDET2 and evidence of the presence of two isoenzymes.
  Journal
J Steroid Biochem Mol Biol 96:287-99 (2005)
DOI:10.1016/j.jsbmb.2005.04.035
  Sequence
[sly:543801]
Other DBs
ExplorEnz - The Enzyme Database: 1.3.1.22
IUBMB Enzyme Nomenclature: 1.3.1.22
ExPASy - ENZYME nomenclature database: 1.3.1.22
BRENDA, the Enzyme Database: 1.3.1.22
CAS: 37255-34-8

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