KEGG   ENZYME: 1.3.1.3Help
Entry
EC 1.3.1.3                  Enzyme                                 

Name
Delta4-3-oxosteroid 5beta-reductase;
3-oxo-Delta4-steroid 5beta-reductase;
5beta-reductase;
androstenedione 5beta-reductase;
cholestenone 5beta-reductase;
cortisone 5beta-reductase;
cortisone beta-reductase;
cortisone Delta4-5beta-reductase;
steroid 5beta-reductase;
testosterone 5beta-reductase;
Delta4-3-ketosteroid 5beta-reductase;
Delta4-5beta-reductase;
Delta4-hydrogenase;
4,5beta-dihydrocortisone:NADP+ Delta4-oxidoreductase;
3-oxo-5beta-steroid:NADP+ Delta4-oxidoreductase
Class
Oxidoreductases;
Acting on the CH-CH group of donors;
With NAD+ or NADP+ as acceptor
BRITE hierarchy
Sysname
5beta-cholestan-3-one:NADP+ 4,5-oxidoreductase
Reaction(IUBMB)
(1) 5beta-cholestan-3-one + NADP+ = cholest-4-en-3-one + NADPH + H+ [RN:R02609];
(2) 17,21-dihydroxy-5beta-pregnane-3,11,20-trione + NADP+ = cortisone + NADPH + H+ [RN:R02893]
Reaction(KEGG)
Substrate
5beta-cholestan-3-one [CPD:C03091];
NADP+ [CPD:C00006];
17,21-dihydroxy-5beta-pregnane-3,11,20-trione
Product
cholest-4-en-3-one [CPD:C00599];
NADPH [CPD:C00005];
H+ [CPD:C00080];
cortisone [CPD:C00762]
Comment
The enzyme from human efficiently catalyses the reduction of progesterone, androstenedione, 17alpha-hydroxyprogesterone and testosterone to 5beta-reduced metabolites; it can also act on aldosterone, corticosterone and cortisol, but to a lesser extent [8]. The bile acid intermediates 7alpha,12alpha-dihydroxy-4-cholesten-3-one and 7alpha-hydroxy-4-cholesten-3-one can also act as substrates [9].
History
EC 1.3.1.3 created 1961 (EC 1.3.1.23 created 1972, incorporated 2005), modified 2005
Pathway
ec00120  Primary bile acid biosynthesis
ec00140  Steroid hormone biosynthesis
ec01100  Metabolic pathways
Orthology
K00251  3-oxo-5-beta-steroid 4-dehydrogenase
K22419  Delta4-3-oxosteroid 5beta-reductase
Genes
HSA: 6718(AKR1D1)
PTR: 472607(AKR1D1)
PPS: 100985077(AKR1D1)
GGO: 101127761(AKR1D1)
PON: 100456267(AKR1D1)
NLE: 100596284(AKR1D1)
MCC: 709863(AKR1D1)
MCF: 102146771(AKR1D1)
CSAB: 103226977(AKR1D1)
RRO: 104656621(AKR1D1)
RBB: 108530143(AKR1D1)
CJC: 100409275(AKR1D1)
SBQ: 101047690(AKR1D1)
MMU: 208665(Akr1d1)
RNO: 192242(Akr1d1)
CGE: 100770348(Akr1d1)
NGI: 103726081(Akr1d1)
HGL: 101716388(Akr1d1)
CCAN: 109700596(Akr1d1)
OCU: 100008612(AKR1D1) 100343388
TUP: 102473225(AKR1D1)
AML: 100483237(AKR1D1)
UMR: 103669050(AKR1D1)
ORO: 101385978(AKR1D1)
FCA: 101100551(AKR1D1)
PTG: 102949203(AKR1D1)
AJU: 106970424(AKR1D1)
BTA: 513855(AKR1D1)
BOM: 102264964(AKR1D1)
BIU: 109557677(AKR1D1)
PHD: 102322934(AKR1D1)
CHX: 102170484(AKR1D1)
OAS: 101103641(AKR1D1)
SSC: 100520813(AKR1D1)
CFR: 102510401(AKR1D1)
CDK: 105091623(AKR1D1)
BACU: 102998601(AKR1D1)
LVE: 103071980(AKR1D1)
OOR: 101283232(AKR1D1)
ECB: 100066919(AKR1D1)
EPZ: 103541705(AKR1D1)
EAI: 106829989(AKR1D1)
MYB: 102243036(AKR1D1) 102246475
HAI: 109388534(AKR1D1)
RSS: 109441506 109457600(AKR1D1)
PALE: 102882463(AKR1D1)
LAV: 100665738(AKR1D1)
TMU: 101348700
MDO: 100022753(AKR1D1)
SHR: 100926677(AKR1D1)
OAA: 100076664
GGA: 418107(AKR1D1)
MGP: 100547967(AKR1D1)
CJO: 107316041(AKR1D1)
APLA: 101802488(AKR1D1)
ACYG: 106031015(AKR1D1)
TGU: 100228769(AKR1D1)
GFR: 102038244(AKR1D1)
FAB: 101820121(AKR1D1)
PHI: 102107003(AKR1D1)
PMAJ: 107204921(AKR1D1)
CCAE: 111937017(AKR1D1)
CCW: 104696483(AKR1D1)
FPG: 101910289(AKR1D1)
FCH: 102047333(AKR1D1)
CLV: 102085311(AKR1D1)
EGZ: 104135569(AKR1D1)
AAM: 106488268(AKR1D1)
ASN: 102377874(AKR1D1)
AMJ: 102564670(AKR1D1)
PSS: 102453451(AKR1D1)
CMY: 102942570(AKR1D1)
CPIC: 101950885(AKR1D1)
ACS: 100555605(akr1d1)
PVT: 110082711(AKR1D1)
PBI: 103055499(AKR1D1) 103060010
GJA: 107115496(AKR1D1)
XLA: 399023 734699(akr1d1.L)
XTR: 594997(akr1d1)
NPR: 108799286(AKR1D1)
IPU: 100528314(akr1d1)
TRU: 101070818(akr1d1)
MALB: 109974454(akr1d1)
SASA: 100286547(akr1d1)
OTW: 112218178(akr1d1)
SFM: 108923648 108935900(akr1d1)
LCM: 102361835(AKR1D1)
ATH: AT4G24220(VEP1)
EGR: 104447829
LJA: Lj0g3v0358119.1(Lj0g3v0358119.1) Lj4g3v2270830.1(Lj4g3v2270830.1)
CSV: 101204090
CMO: 103489839
OSA: 107275612
DOSA: Os03t0422600-00(Os03g0422600) Os03t0436400-01(Os03g0436400) Os07t0601600-00(Os07g0601600) Os11t0351555-01(Os11g0351555)
BDI: 100833464
ATS: 109748315(LOC109748315) 109748316(LOC109748316) 109766846(LOC109766846) 109768392(LOC109768392)
PPP: 112274793
 » show all
Taxonomy
Reference
1  [PMID:13376613]
  Authors
DORFMAN RI, FORCHIELLI E.
  Title
Separation of delta 4-5 alpha-hydrogenases from rat liver homogenates.
  Journal
J Biol Chem 223:443-8 (1956)
Reference
2  [PMID:13805063]
  Authors
BROWN-GRANT K, FORCHIELLI E, DORFMAN RI.
  Title
The delta4-hydrogenases of guinea pig adrenal gland.
  Journal
J Biol Chem 235:1317-20 (1960)
Reference
3
  Authors
Levy, H.R. and Talalay, P.
  Title
Enzymatic introduction of double bonds into steroid ring A.
  Journal
J Am Chem Soc 79:2658-2659 (1957)
Reference
4  [PMID:13416214]
  Authors
TOMKINS GM.
  Title
The enzymatic reduction of delta 4-3-ketosteroids.
  Journal
J Biol Chem 225:13-24 (1957)
Reference
5  [PMID:2278855]
  Authors
Sugimoto Y, Yoshida M, Tamaoki B.
  Title
Purification of 5 beta-reductase from hepatic cytosol fraction of chicken.
  Journal
J Steroid Biochem Mol Biol 37:717-24 (1990)
Reference
6  [PMID:3828348]
  Authors
Furuebisu M, Deguchi S, Okuda K.
  Title
Identification of cortisone 5 beta-reductase as delta 4-3-ketosteroid 5 beta-reductase.
  Journal
Biochim Biophys Acta 912:110-4 (1987)
DOI:10.1016/0167-4838(87)90253-6
Reference
7  [PMID:6736016]
  Authors
Okuda A, Okuda K.
  Title
Purification and characterization of delta 4-3-ketosteroid 5 beta-reductase.
  Journal
J Biol Chem 259:7519-24 (1984)
Reference
8  [PMID:11342103]
  Authors
Charbonneau A, The VL.
  Title
Genomic organization of a human 5beta-reductase and its pseudogene and substrate selectivity of the expressed enzyme.
  Journal
Biochim Biophys Acta 1517:228-35 (2001)
DOI:10.1016/S0167-4781(00)00278-5
  Sequence
[hsa:6718]
Reference
9  [PMID:7508385]
  Authors
Kondo KH, Kai MH, Setoguchi Y, Eggertsen G, Sjoblom P, Setoguchi T, Okuda KI, Bjorkhem I.
  Title
Cloning and expression of cDNA of human delta 4-3-oxosteroid 5 beta-reductase and substrate specificity of the expressed enzyme.
  Journal
Eur J Biochem 219:357-63 (1994)
  Sequence
[hsa:6718]
Other DBs
ExplorEnz - The Enzyme Database: 1.3.1.3
IUBMB Enzyme Nomenclature: 1.3.1.3
ExPASy - ENZYME nomenclature database: 1.3.1.3
BRENDA, the Enzyme Database: 1.3.1.3
CAS: 9029-08-7

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