KEGG   ENZYME: 1.3.1.85Help
Entry
EC 1.3.1.85                 Enzyme                                 

Name
crotonyl-CoA carboxylase/reductase;
CCR;
crotonyl-CoA reductase (carboxylating)
Class
Oxidoreductases;
Acting on the CH-CH group of donors;
With NAD+ or NADP+ as acceptor
BRITE hierarchy
Sysname
(2S)-ethylmalonyl-CoA:NADP+ oxidoreductase (decarboxylating)
Reaction(IUBMB)
(2S)-ethylmalonyl-CoA + NADP+ = (E)-but-2-enoyl-CoA + CO2 + NADPH + H+ [RN:R09291]
Reaction(KEGG)
Substrate
(2S)-ethylmalonyl-CoA [CPD:C18026];
NADP+ [CPD:C00006]
Product
(E)-but-2-enoyl-CoA [CPD:C00877];
CO2 [CPD:C00011];
NADPH [CPD:C00005];
H+ [CPD:C00080]
Comment
The reaction is catalysed in the reverse direction. This enzyme, isolated from the bacterium Rhodobacter sphaeroides, catalyses (E)-but-2-enoyl-CoA-dependent oxidation of NADPH in the presence of CO2. When CO2 is absent, the enzyme catalyses the reduction of (E)-but-2-enoyl-CoA to butanoyl-CoA, but with only 10% of maximal activity (relative to (E)-but-2-enoyl-CoA carboxylation).
History
EC 1.3.1.85 created 2011
Pathway
ec00630  Glyoxylate and dicarboxylate metabolism
ec01120  Microbial metabolism in diverse environments
Orthology
K14446  crotonyl-CoA carboxylase/reductase
Genes
ECA: ECA0600(cfa8B)
PATR: EV46_04860
PATO: GZ59_09850(cfa8B)
PAY: PAU_02213
PST: PSPTO_4689(cfa8)
PAMG: BKM19_030840(ccrA)
PPV: NJ69_15265
OME: OLMES_2177
BVE: AK36_5943(ccrA)
BAM: Bamb_6221
BCON: NL30_35040
PLA: Plav_1621
RBS: RHODOSMS8_02646(ccrA2)
BOS: BSY19_2139(ccrA)
XAU: Xaut_1195
AZC: AZC_0997
MEX: Mext_0288
MEA: Mex_1p0178(ccr)
MDI: METDI0161(ccr)
MCH: Mchl_0332
MPO: Mpop_0364
MET: M446_0037
MNO: Mnod_0039
MOR: MOC_1328(ccrA)
META: Y590_00895
MAQU: Maq22A_1p32000(ccrA)
MEE: DA075_05075(ccrA)
METD: C0214_01265(ccrA)
HDN: Hden_0216
HMC: HYPMC_0098(ccr)
RVA: Rvan_2964
FIL: BN1229_v1_1373(ccr)
FIY: BN1229_v1_1372(ccr)
BVR: BVIR_87
MSC: BN69_1948
MTW: CQW49_03790(ccrA)
MCG: GL4_3337
HDI: HDIA_3961(ccrA2)
RBM: TEF_03480
CCR: CC_3085
CAK: Caul_1067
CSE: Cseg_3646
CMB: CSW64_02210(ccrA)
SIL: SPO0370(ccrA)
RSP: RSP_0960
RCP: RCAP_rcc03243(ccrA)
JAN: Jann_0793
RDE: RD1_1637(ccrA)
RLI: RLO149_c030710(ccr)
PDE: Pden_3873
PYE: A6J80_12555(ccrA)
PZH: CX676_11125(ccrA)
PARO: CUV01_03100(ccrA)
PMUT: DPM13_11015(ccrA)
DSH: Dshi_2853
PSF: PSE_0115
PGD: Gal_03115
OAT: OAN307_c41350(ccr)
OAR: OA238_c04430(ccr)
OTM: OSB_04920(ccrA2)
PTP: RCA23_c09190(ccr)
CID: P73_3852
CEH: CEW89_10025(ccrA)
MALG: MALG_02723
RSU: NHU_00739
RHC: RGUI_0609
LABR: CHH27_17725(ccrA)
YPAC: CEW88_11095(ccrA)
SPSE: SULPSESMR1_00409(ccrA2)
SULZ: C1J03_18095(ccrA)
SULI: C1J05_18020(ccrA)
RMM: ROSMUCSMR3_01832(ccrA2)
LVS: LOKVESSMR4R_02786(ccrA2)
AHT: ANTHELSMS3_02145(ccr)
RBG: BG454_11885(ccrA)
SAGU: CDO87_04300(ccrA)
THAS: C6Y53_10565(ccrA)
GEH: HYN69_12810(ccrA)
HNE: HNE_3118(ccrA)
HBA: Hbal_0288
RRU: Rru_A3063
RRF: F11_15690
MAG: amb0309
MAGX: XM1_1237(ccr)
MAGN: WV31_14300
TMO: TMO_0119
TXI: TH3_17595
THAC: CSC3H3_16685(ccrA)
TII: DY252_04210(ccrA)
MAGQ: MGMAQ_3100
PHR: C6569_14500(ccrA)
CSR: Cspa_c03820(ccr)
RCH: RUM_00790
NTP: CRH09_38255(ccrA)
ROP: ROP_72100
SMA: SAVERM_414(pteB)
SBH: SBI_01373
SALB: XNR_5889
NDA: Ndas_0488
NAL: B005_3356
CWO: Cwoe_4182
LIL: LA_0296(adhP)
LIE: LIF_A0249(adhP)
LIC: LIC_10253
LIS: LIL_10251(adhP1)
LBJ: LBJ_2607
LBL: LBL_0505
LBF: LBF_3008
LST: LSS_00520
LMAY: DPV73_07740(ccrA)
 » show all
Taxonomy
Reference
1  [PMID:17548827]
  Authors
Erb TJ, Berg IA, Brecht V, Muller M, Fuchs G, Alber BE
  Title
Synthesis of C5-dicarboxylic acids from C2-units involving crotonyl-CoA carboxylase/reductase: the ethylmalonyl-CoA pathway.
  Journal
Proc Natl Acad Sci U S A 104:10631-6 (2007)
DOI:10.1073/pnas.0702791104
  Sequence
[rsp:RSP_0960]
Reference
2  [PMID:19458256]
  Authors
Erb TJ, Brecht V, Fuchs G, Muller M, Alber BE
  Title
Carboxylation mechanism and stereochemistry of crotonyl-CoA carboxylase/reductase, a carboxylating enoyl-thioester reductase.
  Journal
Proc Natl Acad Sci U S A 106:8871-6 (2009)
DOI:10.1073/pnas.0903939106
Other DBs
ExplorEnz - The Enzyme Database: 1.3.1.85
IUBMB Enzyme Nomenclature: 1.3.1.85
ExPASy - ENZYME nomenclature database: 1.3.1.85
BRENDA, the Enzyme Database: 1.3.1.85

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