KEGG   ENZYME: 1.3.2.3Help
Entry
EC 1.3.2.3                  Enzyme                                 

Name
L-galactonolactone dehydrogenase;
galactonolactone dehydrogenase;
L-galactono-gamma-lactone dehydrogenase;
L-galactono-gamma-lactone:ferricytochrome-c oxidoreductase;
GLDHase;
GLDase
Class
Oxidoreductases;
Acting on the CH-CH group of donors;
With a cytochrome as acceptor
BRITE hierarchy
Sysname
L-galactono-1,4-lactone:ferricytochrome-c oxidoreductase
Reaction(IUBMB)
L-galactono-1,4-lactone + 4 ferricytochrome c = L-dehydroascorbate + 4 ferrocytochrome c + 4 H+ (overall reaction) [RN:R12144];
(1a) L-galactono-1,4-lactone + 2 ferricytochrome c = L-ascorbate + 2 ferrocytochrome c + 2 H+ [RN:R00640];
(1b) L-ascorbate + 2 ferricytochrome c = L-dehydroascorbate + 2 ferrocytochrome c + 2 H+ (spontaneous) [RN:R07679]
Reaction(KEGG)
Substrate
L-galactono-1,4-lactone [CPD:C01115];
ferricytochrome c [CPD:C00125];
L-ascorbate [CPD:C00072]
Product
L-dehydroascorbate [CPD:C05422];
ferrocytochrome c [CPD:C00126];
H+ [CPD:C00080];
L-ascorbate [CPD:C00072]
Comment
This enzyme catalyses the final step in the biosynthesis of L-ascorbic acid in higher plants and in nearly all higher animals with the exception of primates and some birds [5]. The enzyme is very specific for its substrate L-galactono-1,4-lactone as D-galactono-gamma-lactone, D-gulono-gamma-lactone, L-gulono-gamma-lactone, D-erythronic-gamma-lactone, D-xylonic-gamma-lactone, L-mannono-gamma-lactone, D-galactonate, D-glucuronate and D-gluconate are not substrates [5]. FAD, NAD+, NADP+ and O2 (cf. EC 1.3.3.12, L-galactonolactone oxidase) cannot act as electron acceptor [5].
History
EC 1.3.2.3 created 1961, modified 2006
Pathway
ec00053  Ascorbate and aldarate metabolism
ec01100  Metabolic pathways
ec01110  Biosynthesis of secondary metabolites
Orthology
K00225  L-galactono-1,4-lactone dehydrogenase
Genes
ATH: AT3G47930(GLDH)
ALY: ARALYDRAFT_485156(ATGLDH)
CRB: 17884875
CSAT: 104711271 104780602 104791004
EUS: EUTSA_v10010214mg
BRP: 103873188(GLDH)
BNA: 106389550 106410745 106449166 111211168
BOE: 106335860
THJ: 104816408
CPAP: 110822775
CIT: 102611546
TCC: 18598808
GRA: 105761589
DZI: 111311469
EGR: 104444330
VRA: 106772555
VAR: 108326445
CCAJ: 109805599
CAM: 101498593
LJA: Lj0g3v0304529.1(Lj0g3v0304529.1) Lj5g3v0931500.1(Lj5g3v0931500.1)
ADU: 107474610
AIP: 107625443
LANG: 109336341
FVE: 101296499
PPER: 18769548
PMUM: 103339235
PAVI: 110770247
CSV: 101215955
CMO: 103497260
MCHA: 111021307
CMAX: 111465966
CPEP: 111781077
RCU: 8286649
JCU: 105645077
HBR: 110672781
VVI: 100233005
SLY: 544206(gldh)
SPEN: 107002402
SOT: 102577860(GLDH)
CANN: 107843896(GLDH1)
NSY: 104221614
NTO: 104110446
INI: 109182270
SIND: 105159882
HAN: 110897681
LSV: 111904091
CCAV: 112512576
DCR: 108227887
BVG: 104889449
NNU: 104608844
DOSA: Os11t0143500-01(Os11g0143500) Os12t0139600-01(Os12g0139600)
BDI: 100840661
ATS: 109745724(LOC109745724)
SBI: 110435654
ZMA: 100381436(cl277_1)
PDA: 103720720
EGU: 105045546
MUS: 103988691
PEQ: 110018206
AOF: 109827193
ATR: 18433193
PPP: 112290963
CRE: CHLREDRAFT_168454(GLDH)
APRO: F751_5711
SMIN: v1.2.008428.t1(symbB.v1.2.008428.t1)
SPAR: SPRG_04571
 » show all
Taxonomy
Reference
1
  Authors
Mapson, L.W. and Breslow, E.
  Title
Properties of partially purified L-galactono-gamma-lactone dehydrogenase.
  Journal
Biochem J 65:29 (1957)
Reference
2  [PMID:13126087]
  Authors
MAPSON LW, ISHERWOOD FA, CHEN YT.
  Title
Biological synthesis of L-ascorbic acid: the conversion of L-galactono-gamma-lactone into L-ascorbic acid by plant mitochondria.
  Journal
Biochem J 56:21-8 (1954)
Reference
3  [PMID:13126085]
  Authors
ISHERWOOD FA, CHEN YT, MAPSON LW.
  Title
Synthesis of L-ascorbic acid in plants and animals.
  Journal
Biochem J 56:1-15 (1954)
Reference
4  [PMID:7775377]
  Authors
Oba K, Ishikawa S, Nishikawa M, Mizuno H, Yamamoto T.
  Title
Purification and properties of L-galactono-gamma-lactone dehydrogenase, a key enzyme for ascorbic acid biosynthesis, from sweet potato roots.
  Journal
J Biochem (Tokyo) 117:120-4 (1995)
Reference
5  [PMID:9374475]
  Authors
Ostergaard J, Persiau G, Davey MW, Bauw G, Van Montagu M.
  Title
Isolation of a cDNA coding for L-galactono-gamma-lactone dehydrogenase, an enzyme involved in the biosynthesis of ascorbic acid in plants. Purification, characterization, cDNA cloning, and expression in yeast.
  Journal
J Biol Chem 272:30009-16 (1997)
DOI:10.1074/jbc.272.48.30009
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 1.3.2.3
IUBMB Enzyme Nomenclature: 1.3.2.3
ExPASy - ENZYME nomenclature database: 1.3.2.3
BRENDA, the Enzyme Database: 1.3.2.3
CAS: 9029-02-1

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