KEGG   ENZYME: 1.3.5.5Help
Entry
EC 1.3.5.5                  Enzyme                                 

Name
15-cis-phytoene desaturase;
phytoene desaturase (ambiguous);
PDS;
plant-type phytoene desaturase
Class
Oxidoreductases;
Acting on the CH-CH group of donors;
With a quinone or related compound as acceptor
BRITE hierarchy
Sysname
15-cis-phytoene:plastoquinone oxidoreductase
Reaction(IUBMB)
15-cis-phytoene + 2 plastoquinone = 9,15,9'-tricis-zeta-carotene + 2 plastoquinol (overall reaction) [RN:R09652];
(1a) 15-cis-phytoene + plastoquinone = 15,9'-dicis-phytofluene + plastoquinol [RN:R09653];
(1b) 15,9'-dicis-phytofluene + plastoquinone = 9,15,9'-tricis-zeta-carotene + plastoquinol [RN:R09654]
Reaction(KEGG)
Substrate
15-cis-phytoene [CPD:C05421];
plastoquinone [CPD:C02061];
15,9'-dicis-phytofluene [CPD:C19765]
Product
9,15,9'-tricis-zeta-carotene [CPD:C19764];
plastoquinol [CPD:C16693];
15,9'-dicis-phytofluene [CPD:C19765]
Comment
This enzyme is involved in carotenoid biosynthesis in plants and cyanobacteria. The enzyme from Synechococcus can also use NAD+ and NADP+ as electron acceptor under anaerobic conditions. The enzyme from Gentiana lutea shows no activity with NAD+ or NADP+ [1].
History
EC 1.3.5.5 created 2011
Pathway
ec00906  Carotenoid biosynthesis
ec01100  Metabolic pathways
ec01110  Biosynthesis of secondary metabolites
Orthology
K02293  15-cis-phytoene desaturase
Genes
ATH: AT4G14210(PDS3)
ALY: ARALYDRAFT_493490
CRB: 17878253
CSAT: 104719032 104723803 104732250
EUS: EUTSA_v10024795mg
BRP: 103835193 103863556(PDS)
BNA: 106361559 106390766 106439724 106445763
BOE: 106336235 106342038
THJ: 104822231
CPAP: 110807915
CIT: 102577966
TCC: 18602597
GRA: 105777064
EGR: 104414241
GMX: 100127420 547970(PDS1)
VRA: 106778315
VAR: 108318944
CCAJ: 109797685
CAM: 101499699
LJA: Lj0g3v0087419.1(Lj0g3v0087419.1) Lj0g3v0087419.2(Lj0g3v0087419.2) Lj0g3v0087419.3(Lj0g3v0087419.3) Lj0g3v0087419.4(Lj0g3v0087419.4) Lj0g3v0087419.5(Lj0g3v0087419.5)
ADU: 107482407
AIP: 107638266
FVE: 101297926
PPER: 18788299
PMUM: 103322563
PAVI: 110747173
PXB: 103960366
ZJU: 107422207
CSV: 101209155
CMO: 103494596(pds)
MCHA: 111025543
RCU: 8280960
JCU: 105639092
HBR: 110672897
JRE: 108996675
VVI: 100240685(PDS1)
SLY: 544073(pds)
SPEN: 107014359
SOT: 102577582(PDS)
CANN: 107861625(PDS)
NSY: 104248773
NTO: 104107035
INI: 109192580
OEU: 111400390
HAN: 110913080
LSV: 111915314
BVG: 104899658
SOE: 110787181
NNU: 104589429
OSA: 4331854
DOSA: Os03t0184000-00(Os03g0184000)
OBR: 102713335
BDI: 100830664(PDS)
ATS: 109735159(LOC109735159)
SBI: 110436156
SITA: 101771481
PDA: 103705191
EGU: 105056650
MUS: 103973843(PDS)
DCT: 110108194
AOF: 109848301
ATR: 18427456
PPP: 112274368
CRE: CHLREDRAFT_78128(PDS1)
MIS: MICPUN_104873(PDS1) MICPUN_96647(PDS2)
APRO: F751_0480
SMIN: v1.2.025723.t1(symbB.v1.2.025723.t1)
SVA: SVA_2656
SYN: slr1254(pds)
SYZ: MYO_112820(pds)
SYY: SYNGTS_1271(pds)
SYT: SYNGTI_1271(pds)
SYS: SYNPCCN_1270(pds)
SYQ: SYNPCCP_1270(pds)
SYJ: D082_22590(pds)
SYO: C7I86_06580(pds)
SYW: SYNW2257(crtD)
SYC: syc2113_c(pds)
SYG: sync_2608
SYR: SynRCC307_0238(crtD)
SYX: SynWH7803_2273(crtD)
SYP: SYNPCC7002_A1935(crtP)
CYA: CYA_0316(pds)
CYB: CYB_1694(pds)
SYNR: KR49_07565
SYND: KR52_04115
SYH: Syncc8109_2504(crtD)
SYNW: SynWH8103_02594(pds)
TEL: tll1561(pds)
THN: NK55_04445(crtP)
CYI: CBM981_3153(pds)
LET: O77CONTIG1_00579(pds_1)
PMA: Pro_0167(pds)
PMM: PMM0144(pds)
PMT: PMT_2004
PMB: A9601_01611(pds)
PMC: P9515_01721(pds)
PMF: P9303_26651(pds)
PMG: P9301_01631(pds)
PMH: P9215_01611(pds)
PMJ: P9211_01591(pds)
PME: NATL1_02161(pds)
PRC: EW14_0186
PRM: EW15_0231
AMR: AM1_4826(crtP)
MAR: MAE_12930(pds)
CYL: AA637_04015(crtP)
CYT: cce_3754
TER: Tery_4011
ARP: NIES39_C00940(crtP)
ANA: alr1832(pds)
NON: NOS3756_19130(pds)
NOE: CLI64_05545(pds)
AVA: Ava_4795
NAZ: Aazo_2875
ANB: ANA_C20304(crtP)
CALH: IJ00_15390
CTHE: Chro_0494
CEO: ETSB_0715
CAU: Caur_2352
CAG: Cagg_3041
OTE: Oter_4135
CTE: CT0807
CPC: Cpar_0836
CCH: Cag_1188
CLI: Clim_0877
PVI: Cvib_1032
PLT: Plut_1283
PPH: Ppha_1176
PAA: Paes_0884
PROC: Ptc2401_00978(pds_2)
CTS: Ctha_1159
 » show all
Taxonomy
Reference
1  [PMID:11714315]
  Authors
Breitenbach J, Zhu C, Sandmann G
  Title
Bleaching herbicide norflurazon inhibits phytoene desaturase by competition with  the cofactors.
  Journal
J Agric Food Chem 49:5270-2 (2001)
DOI:10.1021/jf0106751
Reference
2  [PMID:9226712]
  Authors
Schneider C, Boger P, Sandmann G
  Title
Phytoene desaturase: heterologous expression in an active state, purification, and biochemical properties.
  Journal
Protein Expr Purif 10:175-9 (1997)
DOI:10.1006/prep.1997.0730
  Sequence
Reference
3  [PMID:8489496]
  Authors
Fraser PD, Linden H, Sandmann G
  Title
Purification and reactivation of recombinant Synechococcus phytoene desaturase from an overexpressing strain of Escherichia coli.
  Journal
Biochem J 291 ( Pt 3):687-92 (1993)
Reference
4  [PMID:15503129]
  Authors
Breitenbach J, Sandmann G
  Title
zeta-Carotene cis isomers as products and substrates in the plant poly-cis carotenoid biosynthetic pathway to lycopene.
  Journal
Planta 220:785-93 (2005)
DOI:10.1007/s00425-004-1395-2
Other DBs
ExplorEnz - The Enzyme Database: 1.3.5.5
IUBMB Enzyme Nomenclature: 1.3.5.5
ExPASy - ENZYME nomenclature database: 1.3.5.5
BRENDA, the Enzyme Database: 1.3.5.5

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