KEGG   ENZYME: 1.3.98.1Help
Entry
EC 1.3.98.1                 Enzyme                                 

Name
dihydroorotate dehydrogenase (fumarate);
DHOdehase (ambiguous);
dihydroorotate dehydrogenase (ambiguous);
dihydoorotic acid dehydrogenase (ambiguous);
DHOD (ambiguous);
DHODase (ambiguous);
dihydroorotate oxidase, pyr4 (gene name)
Class
Oxidoreductases;
Acting on the CH-CH group of donors;
With other, known, physiological acceptors
BRITE hierarchy
Sysname
(S)-dihydroorotate:fumarate oxidoreductase
Reaction(IUBMB)
(S)-dihydroorotate + fumarate = orotate + succinate [RN:R01867]
Reaction(KEGG)
Substrate
(S)-dihydroorotate [CPD:C00337];
fumarate [CPD:C00122]
Product
orotate [CPD:C00295];
succinate [CPD:C00042]
Comment
Binds FMN. The reaction, which takes place in the cytosol, is the only redox reaction in the de novo biosynthesis of pyrimidine nucleotides. Molecular oxygen can replace fumarate in vitro. Other class 1 dihydroorotate dehydrogenases use either NAD+ (EC 1.3.1.14) or NADP+ (EC 1.3.1.15) as electron acceptor. The membrane bound class 2 dihydroorotate dehydrogenase (EC 1.3.5.2) uses quinone as electron acceptor.
History
EC 1.3.98.1 created 1961 as EC 1.3.3.1, transferred 2011 to EC 1.3.98.1
Pathway
ec00240  Pyrimidine metabolism
ec01100  Metabolic pathways
Orthology
K00226  dihydroorotate dehydrogenase (fumarate)
Genes
SCE: YKL216W(URA1)
KLA: KLLA0_C19360g
KMX: KLMA_10832(URA1)
LTH: KLTH0F00308g
ZRO: ZYRO0D00396g
NCS: NCAS_0D03520(NCAS0D03520)
NDI: NDAI_0H00120(NDAI0H00120)
TPF: TPHA_0K00200(TPHA0K00200)
TBL: TBLA_0B10140(TBLA0B10140)
TDL: TDEL_0F05620(TDEL0F05620)
KAF: KAFR_0L00170(KAFR0L00170)
NCR: NCU04318
NTE: NEUTE1DRAFT15063(NEUTE1DRAFT_15063)
MGR: MGG_07263
SSCK: SPSK_01211
MAW: MAC_06929
MAJ: MAA_00067
BFU: BCIN_16g04110(Bcura1)
PTE: PTT_01403
ABP: AGABI1DRAFT51017(AGABI1DRAFT_51017)
ABV: AGABI2DRAFT214748(AGABI2DRAFT_214748)
SMIN: v1.2.031230.t1(symbB.v1.2.031230.t1) v1.2.031230.t2(symbB.v1.2.031230.t2)
SPAR: SPRG_07872
LMA: LMJF_16_0530(DHODH)
LIF: LINJ_16_0540(DHODH)
LBZ: LBRM_16_0550(DHODH)
EAM: EAMY_0442
EAY: EAM_2979
PLU: plu3917
HSO: HS_0799
HSM: HSM_1268
MHT: D648_7350
MHAT: B824_7980
MHAE: F382_11785
MHAM: J450_10470
MHAO: J451_11895
MHAL: N220_03905
MHAQ: WC39_09975
MHAY: VK67_09975
MVI: X808_6700
MVG: X874_6790
BTO: WQG_17660
BTRE: F542_4910
BTRH: F543_5590
BTRA: F544_17520
SVO: SVI_3253(pyrD-2)
SPSW: Sps_04212
MAD: HP15_3391
MCA: MCA0768
TBN: TBH_C0805
ECOR: SAMEA4412678_1339(pyrDA)
CVI: CV_3551(pydA)
LHK: LHK_00854
TBD: Tbd_2467
DAR: Daro_2839
SULR: B649_05420
DPS: DP0769
DML: Dmul_38520(pyrD2)
ADE: Adeh_0762
RPB: RPB_3115
MSL: Msil_2988
PZU: PHZ_p0133
RCP: RCAP_rcc01727(pyrD1)
ASZ: ASN_2514
MAG: amb2359
MAGX: XM1_3143
MAGN: WV31_07695
SAU: SA2375
SAV: SAV2589
SAW: SAHV_2573
SAM: MW2509
SAS: SAS2475
SAR: SAR2669
SAC: SACOL2606(pyrD)
SAX: USA300HOU_2583(pyrD)
SAA: SAUSA300_2526(pyrD)
SAE: NWMN_2488(pyrD)
SAD: SAAV_2655(pyrD)
SUE: SAOV_2634
SUJ: SAA6159_02486(pyrD)
SUK: SAA6008_02648(pyrD)
SUC: ECTR2_2443(pyrD)
SUQ: HMPREF0772_10602(pyrD)
SUZ: MS7_2595(pyrD)
SUG: SAPIG2639(pyrD)
SAUA: SAAG_00409
SAUE: RSAU_002430(pyrD)
SAUS: SA40_2343
SAUU: SA957_2427
SAUG: SA268_2505
SAUF: X998_2573
SAB: SAB2464
SAUB: C248_2650
SAUC: CA347_2666(pyrD)
SAUR: SABB_02002
SAUI: AZ30_13560
SAUD: CH52_06060
SAMS: NI36_12960
SEP: SE2132
SER: SERP2144(pyrD)
SEPP: SEB_02136
SEPS: DP17_1072(pyrD)
SHA: SH0471
SHH: ShL2_00375(pyrD)
SSP: SSP0278
SPAS: STP1_1082
SXO: SXYL_00310(pyrD)
SCAP: AYP1020_1752(pyrD)
GMO: NCTC11323_00512(pyrDA)
LLA: L192589(pydA)
LLK: LLKF_1674(pydA)
LLT: CVCAS_1457(pydA)
LLS: lilo_1474(pydA)
LLX: NCDO2118_1571(pyrDA)
LLC: LACR_1644
LLM: llmg_0952(pyrDA)
LLR: llh_4795
LLI: uc509_1504(pyrDA)
LLW: kw2_1506
LLJ: LG36_1498(pydA)
LGR: LCGT_1508
LGV: LCGL_1530
SPY: SPy_1432(pyrD)
SPZ: M5005_Spy1165(pyrD)
SPYM: M1GAS476_1227(pyrD)
SPYA: A20_1200(pyrD)
SPG: SpyM3_1091(pyrD)
SPS: SPs0774
SPH: MGAS10270_Spy1236(pyrD)
SPI: MGAS10750_Spy1273(pyrD)
SPJ: MGAS2096_Spy1237(pyrD)
SPK: MGAS9429_Spy1214(pyrD)
SPF: SpyM50695(pyrD)
SPB: M28_Spy1159(pyrD)
STG: MGAS15252_1104(pyrD)
STX: MGAS1882_1099(pyrD)
SOZ: Spy49_1144(pyrD)
STZ: SPYALAB49_001164(pyrD)
SPYH: L897_05790
SPN: SP_0764(pyrDA)
SPD: SPD_0665(pyrDa)
SPR: spr0672(pyrDA)
SPW: SPCG_0713
SJJ: SPJ_0701
SNV: SPNINV200_06750(pyrD)
SPX: SPG_0695
SNT: SPT_0777
SND: MYY_0796
SPNN: T308_03565
SNE: SPN23F06860(pyrD)
SPV: SPH_0863
SPP: SPP_0773
SNI: INV104_06350(pyrD)
SPNG: HMPREF1038_00774(pyrDA)
SNP: SPAP_0737
SNX: SPNOXC06910(pyrD)
SNU: SPNA45_01088(pyrD)
SPNE: SPN034156_17400(pyrD)
SPNU: SPN034183_06920(pyrD)
SPNM: SPN994038_06810(pyrD)
SPNO: SPN994039_06820(pyrD)
SAG: SAG0507(pyrDA)
SAN: gbs0553
SAK: SAK_0657(pyrD)
SGC: A964_0538(pyrD)
SAGS: SaSA20_0492(pyrD)
SAGL: GBS222_0484(pyrD)
SAGM: BSA_5950
SAGI: MSA_6100
SAGR: SAIL_6240
SAGP: V193_02825
SAGC: DN94_02825
SAGE: EN72_02920
SAGG: EN73_02995
SAGN: W903_0684
SMU: SMU_595(pyrD)
SMC: SmuNN2025_1383(pyrD)
SMJ: SMULJ23_1390(pyrD)
SMUA: SMUFR_0516(pyrD)
STC: str1207(pyrDa)
STL: stu1207(pyrDa)
STE: STER_1173
STN: STND_1143
STU: STH8232_1412(pyrD2)
STW: Y1U_C1109
STHE: T303_06890
SSA: SSA_0373(pyrDA)
SSB: SSUBM407_0226(pyrD)
SSI: SSU0235(pyrD)
SSS: SSUSC84_0224(pyrD)
SUP: YYK_01095
SSUY: YB51_1255
SSUT: TL13_0279(pyrD)
SSUI: T15_0241(pyrD)
SGO: SGO_0277(pyrA)
SEZ: Sez_0633
SEQ: SZO_13640
SEZO: SeseC_00765(pyrD)
SEQU: Q426_06160
SEU: SEQ_0655
SUB: SUB1264(pyrD)
SDS: SDEG_1471(pyrD)
SDA: GGS_1341(pyrD)
SDC: SDSE_1579(pyrD)
SDQ: SDSE167_1579(pyrD)
SGA: GALLO_0639(pyrD)
SGG: SGGBAA2069_c05820(pyrD)
SGT: SGGB_0614(pyrD1)
SMB: smi_0815(pyrD2)
SOR: SOR_0779(pyrD)
STK: STP_1098
STB: SGPB_0508(pyrD.1)
SCF: Spaf_1800(pyrD3)
SSR: SALIVB_1200(pyrD)
STF: Ssal_01281(pyrD)
STJ: SALIVA_0882(pyrD)
SSAH: HSISS4_00782(pyrDa)
SMN: SMA_0601(pyrD)
SIF: Sinf_0520(pyrD)
SIE: SCIM_0207(pyrD)
SIB: SIR_0264(pyrDa)
SIU: SII_0247(pyrDa)
SANG: SAIN_1602(pyrDa)
SANC: SANR_1834(pyrDa)
SANS: DK43_01485
SCG: SCI_0264(pyrDa)
SCON: SCRE_0244(pyrDa)
SCOS: SCR2_0244(pyrDa)
SIK: K710_0597
SLU: KE3_0599
STRN: SNAG_0804(pyrDA)
LJO: LJ_1281
LJF: FI9785_918(pyrD)
LJH: LJP_0899
LAC: LBA1384(pyrD)
LAD: LA14_1382
LAF: SD55_1367(pyrD)
LSA: LCA_0446
LSL: LSL_0194(pyrD)
LSI: HN6_00184(pyrD)
LSJ: LSJ_0237(pyrD)
LDB: Ldb1530(pyrD2) Ldb2114(pyrD1)
LDE: LDBND_1465(pyrD2)
LCA: LSEI_1732
LPAP: LBPC_1652
LCB: LCABL_19520(pyrDA)
LCS: LCBD_1929
LCE: LC2W_1908
LCW: BN194_19150(pyrDA)
LGA: LGAS_1092
LHE: lhv_2937
LHL: LBHH_0706
LHV: lhe_1366
LHH: LBH_1217
LHD: HUO_04845
LFE: LAF_1201
LFR: LC40_0780
LFF: LBFF_1312
LRH: LGG_01789(pyrD)
LRG: LRHM_1725
LRL: LC705_01772(pyrD)
LRA: LRHK_1764(ura1)
LCR: LCRIS_01374(pyrD)
LAM: LA2_07985
LBH: Lbuc_0385
LBN: LBUCD034_0423(pyrD)
LKE: WANG_0334
LRM: LRC_09270
LSN: LSA_05870(pyrDA)
LAE: LBAT_0593
EFA: EF0285(pyrD-1)
EFL: EF62_0674(pyrD-1)
EFI: OG1RF_10228(pyrDA)
EFS: EFS1_0226(pyrD-1)
EFN: DENG_00270(pyrD)
EFQ: DR75_2333(ura1)
EFC: EFAU004_00893(pyrDA)
EFAU: EFAU085_01453(pyrDA)
EFU: HMPREF0351_11431(pyrD2)
EFM: M7W_1926
EHR: EHR_11760
ECAS: ECBG_02253
EMU: EMQU_1428(pyrDA)
EDU: LIU_08370
THL: TEH_14370(pyrD)
OOE: OEOE_0264
LME: LEUM_1279
LMM: MI1_05585
LMK: LMES_1061
LKI: LKI_09935
LGS: LEGAS_1084(pyrDA)
WCE: WS08_0824
WCT: WS74_0890
CBI: CLJ_0029
CDF: CD630_20770(pyrD)
PDC: CDIF630_02300(preA1)
CDC: CD196_1941(pyrD)
CDL: CDR20291_1984(pyrD)
PDF: CD630DERM_20770(pyrD1)
MMC: Mmcs_4864
MKM: Mkms_4953
MJL: Mjls_5232
MMI: MMAR_3409
MVA: Mvan_4110
SBH: SBI_02815
PAC: PPA0161
PAW: PAZ_c01720(pyrD1)
PACC: PAC1_00855
PACH: PAGK_0189
CACN: RN83_01250
CGRN: 4412665_00536(pyrD)
ACIJ: JS278_02796(preA)
TFU: Tfu_2378
FAL: FRAAL1902
BLO: BL1213
BLJ: BLD_0976(pyrD2)
BLN: Blon_2065
BLON: BLIJ_2142
BLF: BLIF_0413
BLL: BLJ_0451
BLB: BBMN68_979(pyrD2)
BLM: BLLJ_0396
BLG: BIL_14510
BAD: BAD_1334
BADL: BADO_1392
BLA: BLA_0559
BBB: BIF_00336
BBC: BLC1_1312
BLV: BalV_1312
BLW: W7Y_1359
BLS: W91_1392
BANI: Bl12_1271
BANL: BLAC_06785
BANM: EN10_06860
BDE: BDP_1799(pyrD2)
BDN: BBDE_1702
BBI: BBIF_0429(pyrD2)
BBP: BBPR_0404(pyrD)
BBF: BBB_0380(pyrD)
BBRU: Bbr_0489(pyrD2)
BBRE: B12L_0408
BBRV: B689b_0468
BBRJ: B7017_0444
BBRC: B7019_0442
BBRN: B2258_0442
BBRS: BS27_0480(pyrD2)
BBRD: BBBR_0412
BTP: D805_0440
BKS: BBKW_1566
BPSP: AH67_07345
BANG: BBAG_1255
BCAT: BBCT_1364
BPSC: BBPC_1463
BSCA: BBSC_2076
SIJ: SCIP_1083
PDO: PSDT_0374
SYN: sll0744
SYY: SYNGTS_2808(sll0744)
SYT: SYNGTI_2807(sll0744)
SYS: SYNPCCN_2806(sll0744)
SYQ: SYNPCCP_2806(sll0744)
SYC: syc1720_c
AMR: AM1_D0141(pyrD)
MAR: MAE_38150
MPK: VL20_5511
CYT: cce_3252
ARP: NIES39_L04100(pyrD)
ANA: alr1912
AVA: Ava_3045
ANB: ANA_C11950(pyrD)
CTHE: Chro_4406
STAN: STA3757_05380(pyrD_1)
RCA: Rcas_2064
CAU: Caur_2081
CAG: Cagg_2635
ATM: ANT_28310
TBC: A0O31_00009(pyrD)
OTE: Oter_0606
OBG: Verru16b_00598(preA_1) Verru16b_01854(preA_2)
CAA: Caka_1151
MIN: Minf_1387(pyrD) Minf_2385(pyrD)
RBA: RB4738 RB4752(pyrD) RB9748
PLH: VT85_05270(preA)
FMR: Fuma_01332(preA)
TTF: THTE_4057
VBL: L21SP4_00337(pyrD_1)
ABAS: ACPOL_5878
SUS: Acid_6995
ABAC: LuPra_04112(preA_2)
LBA: Lebu_2234
BTH: BT_1333
BTHO: Btheta7330_01888(preA)
BFR: BF2950
BVU: BVU_3869
BXY: BXY_43000
BOA: Bovatus_01352(preA)
BCEL: BcellWH2_05077(preA)
PGI: PG_2055
PGN: PGN_1999
PCRE: NCTC12858_00853(pyrD_1)
PMUC: ING2E5A_0046(URA1) ING2E5A_0807(pyrD1) ING2E5A_2689(pyrD5)
PDI: BDI_0543
TFO: BFO_0242
AFD: Alfi_1991
BLQ: L21SP5_00505(pyrD_2) L21SP5_00918(preA) L21SP5_01040(pyrDA_1) L21SP5_02712(pyrDA_2)
SGN: SGRA_p0002(pyrD)
EAO: BD94_0307
ELB: VO54_02286(pyrDA)
FBU: UJ101_01334(pyrDI)
BPI: BPLAN_127(pyrD)
BMM: MADAR_234(pyrD)
BCP: BLBCPU_481(pyrD)
BBG: BGIGA_124(pyrD)
BLP: BPAA_124(pyrD)
BLU: K645_2431
CLI: Clim_2445
PVI: Cvib_1724
PLT: Plut_2084
PAA: Paes_2219
PROC: Ptc2401_02228(pyrD_2)
CTS: Ctha_0742
IAL: IALB_2950(pyrD)
MRO: MROS_2662
TLE: Tlet_1878
TME: Tmel_0121
CABY: Cabys_261
DTU: Dtur_0444
NDE: NIDE1091(pyrD)
NMV: NITMOv2_0967(pyrD)
NIO: NITINOP_2763(pyrD)
NJA: NSJP_2098(pyrDA)
SAAL: L336_0761
STO: STK_14770(pyrD)
SSO: SSO0610(pyrD)
SOL: Ssol_1674
SSOA: SULA_1702
SSOL: SULB_1703
SSOF: SULC_1701
SAI: Saci_1592(pyrD)
SID: M164_1519
SII: LD85_1729
SIH: SiH_1488
SIR: SiRe_1397
SIC: SiL_1400
MSE: Msed_1960
MCN: Mcup_0324
AHO: Ahos_1133
PAI: PAE3263
PIS: Pisl_0507
PCL: Pcal_1778
PAS: Pars_1692
PYR: P186_1337
POG: Pogu_0443
TNE: Tneu_1590
CMA: Cmaq_0564
TUZ: TUZN_0505
TTN: TTX_0796(pyrD)
VDI: Vdis_1107
VMO: VMUT_1972
 » show all
Taxonomy
Reference
1  [PMID:9405053]
  Authors
Bjornberg O, Rowland P, Larsen S, Jensen KF
  Title
Active site of dihydroorotate dehydrogenase A from Lactococcus lactis investigated by chemical modification and mutagenesis.
  Journal
Biochemistry 36:16197-205 (1997)
DOI:10.1021/bi971628y
  Sequence
[llm:llmg_0952]
Reference
2  [PMID:9655329]
  Authors
Rowland P, Bjornberg O, Nielsen FS, Jensen KF, Larsen S
  Title
The crystal structure of Lactococcus lactis dihydroorotate dehydrogenase A complexed with the enzyme reaction product throws light on its enzymatic function.
  Journal
Protein Sci 7:1269-79 (1998)
DOI:10.1002/pro.5560070601
  Sequence
[llm:llmg_0952]
Reference
3  [PMID:12732650]
  Authors
Norager S, Arent S, Bjornberg O, Ottosen M, Lo Leggio L, Jensen KF, Larsen S
  Title
Lactococcus lactis dihydroorotate dehydrogenase A mutants reveal important facets of the enzymatic function.
  Journal
J Biol Chem 278:28812-22 (2003)
DOI:10.1074/jbc.M303767200
  Sequence
[llm:llmg_0952]
Reference
4  [PMID:17617217]
  Authors
Zameitat E, Pierik AJ, Zocher K, Loffler M
  Title
Dihydroorotate dehydrogenase from Saccharomyces cerevisiae: spectroscopic investigations with the recombinant enzyme throw light on catalytic properties and metabolism of fumarate analogues.
  Journal
FEMS Yeast Res 7:897-904 (2007)
DOI:10.1111/j.1567-1364.2007.00275.x
  Sequence
[sce:YKL216W]
Reference
5  [PMID:18808149]
  Authors
Inaoka DK, Sakamoto K, Shimizu H, Shiba T, Kurisu G, Nara T, Aoki T, Kita K, Harada S
  Title
Structures of Trypanosoma cruzi dihydroorotate dehydrogenase complexed with substrates and products: atomic resolution insights into mechanisms of dihydroorotate oxidation and fumarate reduction.
  Journal
Biochemistry 47:10881-91 (2008)
DOI:10.1021/bi800413r
  Sequence
[tcr:508375.50]
Reference
6  [PMID:19932077]
  Authors
Cheleski J, Wiggers HJ, Citadini AP, da Costa Filho AJ, Nonato MC, Montanari CA
  Title
Kinetic mechanism and catalysis of Trypanosoma cruzi dihydroorotate dehydrogenase enzyme evaluated by isothermal titration calorimetry.
  Journal
Anal Biochem 399:13-22 (2010)
DOI:10.1016/j.ab.2009.11.018
Other DBs
ExplorEnz - The Enzyme Database: 1.3.98.1
IUBMB Enzyme Nomenclature: 1.3.98.1
ExPASy - ENZYME nomenclature database: 1.3.98.1
BRENDA, the Enzyme Database: 1.3.98.1
CAS: 9029-03-2

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