KEGG   ENZYME: 1.3.99.27Help
Entry
EC 1.3.99.27                Enzyme                                 

Name
1-hydroxycarotenoid 3,4-desaturase;
CrtD;
hydroxyneurosporene desaturase;
carotenoid 3,4-dehydrogenase;
1-hydroxy-carotenoid 3,4-dehydrogenase
Class
Oxidoreductases;
Acting on the CH-CH group of donors;
With unknown physiological acceptors
BRITE hierarchy
Sysname
1-hydroxy-1,2-dihydrolycopene:acceptor oxidoreductase
Reaction(IUBMB)
1-hydroxy-1,2-dihydrolycopene + acceptor = 1-hydroxy-3,4-didehydro-1,2-dihydrolycopene + reduced acceptor [RN:R07520]
Reaction(KEGG)
R07520;
(other) R07523 R07534
Show
Substrate
1-hydroxy-1,2-dihydrolycopene [CPD:C19795];
acceptor [CPD:C00028]
Product
1-hydroxy-3,4-didehydro-1,2-dihydrolycopene [CPD:C15874];
reduced acceptor [CPD:C00030]
Comment
The enzymes from Rubrivivax gelatinosus and Rhodobacter sphaeroides prefer the acyclic carotenoids (e.g. 1-hydroxy-1,2-dihydroneurosporene, 1-hydroxy-1,2-dihydrolycopene) as substrates. The conversion rate for the 3,4-desaturation of the monocyclic 1'-hydroxy-1',2'-dihydro-gamma-carotene is lower [2,3]. The enzyme from the marine bacterium strain P99-3 shows high activity with the monocyclic carotenoid 1'-hydroxy-1',2'-dihydro-gamma-carotene [1]. The enzyme from Rhodobacter sphaeroides utilizes molecular oxygen as the electron acceptor in vitro [3]. However, oxygen is unlikely to be the natural electron acceptor under anaerobic conditions.
History
EC 1.3.99.27 created 2011
Pathway
ec00906  Carotenoid biosynthesis
ec01100  Metabolic pathways
ec01110  Biosynthesis of secondary metabolites
Orthology
K09845  1-hydroxycarotenoid 3,4-desaturase
Genes
ALV: Alvin_2566
TVI: Thivi_2015
TMB: Thimo_3009
MPUR: MARPU_13290
TSY: THSYN_31065 THSYN_31070
HHA: Hhal_1614
HHC: M911_10370
EBS: ECTOBSL9_3050
PDQ: CL55_00013470
RAC: RA876_06050
LIM: L103DPR2_00983(crtD)
LIH: L63ED372_00638(crtD)
HYR: BSY239_1370(crtI)
RGE: RGE_33590(crtD)
METR: BSY238_2435(crtI)
SCL: sce0585(crtD)
AGC: BSY240_3672(crtI)
BRA: BRADO1614(crtD)
BBT: BBta_6441(crtD)
BRS: S23_19140(crtD)
AOL: S58_59220
BRO: BRAD285_5786(crtD)
RPA: RPA1518(crtD)
RPB: RPB_4005
RPC: RPC_1289
RPD: RPD_3760
RPE: RPE_1323
RPT: Rpal_1706
BOS: BSY19_4059(crtI)
MEX: Mext_2726
MDI: METDI3492
MCH: Mchl_2953
MPO: Mpop_2848
MET: M446_3693
MOR: MOC_3187
META: Y590_13120
MSL: Msil_2048
RVA: Rvan_0207
BVR: BVIR_3174
RSP: RSP_0266(crtD)
RCP: RCAP_rcc00683(crtD)
JAN: Jann_0183(crtD)
RDE: RD1_0115(crtD)
RLI: RLO149_p940120(crtD)
DSH: Dshi_3513(crtD)
PTP: RCA23_c29580(crtD)
RSU: NHU_04283
RHC: RGUI_1965
RMM: ROSMUCSMR3_00909(crtD)
LVS: LOKVESSMR4R_03675(crtD)
SPHI: TS85_22890
SSAN: NX02_18465
BLAS: BSY18_2718(crtI)
AMV: ACMV_18830(crtD)
RRU: Rru_A2984
RRF: F11_15285
RCE: RC1_2090(crtD)
RPM: RSPPHO_02323(crtD)
 » show all
Taxonomy
Reference
1  [PMID:15251462]
  Authors
Teramoto M, Rahlert N, Misawa N, Sandmann G.
  Title
1-Hydroxy monocyclic carotenoid 3,4-dehydrogenase from a marine bacterium that produces myxol.
  Journal
FEBS Lett 570:184-8 (2004)
DOI:10.1016/j.febslet.2004.05.085
Reference
2  [PMID:10880364]
  Authors
Steiger S, Astier C, Sandmann G
  Title
Substrate specificity of the expressed carotenoid 3,4-desaturase from Rubrivivax  gelatinosus reveals the detailed reaction sequence to spheroidene and spirilloxanthin.
  Journal
Biochem J 349:635-40 (2000)
  Sequence
[rge:RGE_33590]
Reference
3  [PMID:9393712]
  Authors
Albrecht M, Ruther A, Sandmann G.
  Title
Purification and biochemical characterization of a hydroxyneurosporene desaturase involved in the biosynthetic pathway of the carotenoid spheroidene in Rhodobacter sphaeroides.
  Journal
J Bacteriol 179:7462-7 (1997)
DOI:10.1128/JB.179.23.7462-7467.1997
  Sequence
[rsp:RSP_0266]
Other DBs
ExplorEnz - The Enzyme Database: 1.3.99.27
IUBMB Enzyme Nomenclature: 1.3.99.27
ExPASy - ENZYME nomenclature database: 1.3.99.27
BRENDA, the Enzyme Database: 1.3.99.27

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