KEGG   ENZYME: 1.3.99.30Help
Entry
EC 1.3.99.30                Enzyme                                 

Name
phytoene desaturase (3,4-didehydrolycopene-forming);
5-step phytoene desaturase;
five-step phytoene desaturase;
phytoene desaturase (ambiguous);
Al-1
Class
Oxidoreductases;
Acting on the CH-CH group of donors;
With unknown physiological acceptors
BRITE hierarchy
Sysname
15-cis-phytoene:acceptor oxidoreductase (3,4-didehydrolycopene-forming)
Reaction(IUBMB)
15-cis-phytoene + 5 acceptor = all-trans-3,4-didehydrolycopene + 5 reduced acceptor (overall reaction) [RN:R09703];
(1a) 15-cis-phytoene + acceptor = all-trans-phytofluene + reduced acceptor [RN:R09692];
(1b) all-trans-phytofluene + acceptor = all-trans-zeta-carotene + reduced acceptor [RN:R04787];
(1c) all-trans-zeta-carotene + acceptor = all-trans-neurosporene + reduced acceptor [RN:R04798];
(1d) all-trans-neurosporene + acceptor = all-trans-lycopene + reduced acceptor [RN:R04800];
(1e) all-trans-lycopene + acceptor = all-trans-3,4-didehydrolycopene + reduced acceptor [RN:R07518]
Reaction(KEGG)
Substrate
15-cis-phytoene [CPD:C05421];
acceptor [CPD:C00028];
all-trans-phytofluene [CPD:C05414];
all-trans-zeta-carotene [CPD:C05430];
all-trans-neurosporene [CPD:C05431];
all-trans-lycopene [CPD:C05432]
Product
all-trans-3,4-didehydrolycopene [CPD:C15867];
reduced acceptor [CPD:C00030];
all-trans-phytofluene [CPD:C05414];
all-trans-zeta-carotene [CPD:C05430];
all-trans-neurosporene [CPD:C05431];
all-trans-lycopene [CPD:C05432]
Comment
This enzyme is involved in carotenoid biosynthesis and catalyses up to five desaturation steps (cf. EC 1.3.99.28 [phytoene desaturase (neurosporene-forming)], EC 1.3.99.29 [phytoene desaturase (zeta-carotene-forming)] and EC 1.3.99.31 [phytoene desaturase (lycopene-forming)]).
History
EC 1.3.99.30 created 2011
Pathway
ec00906  Carotenoid biosynthesis
ec01100  Metabolic pathways
ec01110  Biosynthesis of secondary metabolites
Orthology
K15745  phytoene desaturase (3,4-didehydrolycopene-forming)
Genes
API: 100159050 100161380 100169110 100169245(tor)
DNX: 107165429 107165446 107172433
TUT: 107364307 107364309 107365911
VCN: VOLCADRAFT_96696
MNG: MNEG_6064
BPG: Bathy15g02080
MIS: MICPUN_85926
MPP: MICPUCDRAFT_36095
NCR: NCU00552(al-1)
NTE: NEUTE1DRAFT92886(NEUTE1DRAFT_92886)
SMP: SMAC_01244(putative al1)
MBE: MBM_00660
ANG: ANI_1_1482064(An07g01150)
ABE: ARB_03950
TVE: TRV_03238
PTE: PTT_09941
SMIN: v1.2.005646.t1(symbB.v1.2.005646.t1)
 » show all
Taxonomy
Reference
1  [PMID:11017770]
  Authors
Hausmann A, Sandmann G.
  Title
A single five-step desaturase is involved in the carotenoid biosynthesis pathway to beta-carotene and torulene in Neurospora crassa.
  Journal
Fungal Genet Biol 30:147-53 (2000)
DOI:10.1006/fgbi.2000.1212
  Sequence
[ncr:NCU00552]
Reference
2  [PMID:18812228]
  Authors
Estrada AF, Maier D, Scherzinger D, Avalos J, Al-Babili S
  Title
Novel apocarotenoid intermediates in Neurospora crassa mutants imply a new biosynthetic reaction sequence leading to neurosporaxanthin formation.
  Journal
Fungal Genet Biol 45:1497-505 (2008)
DOI:10.1016/j.fgb.2008.09.001
  Sequence
[ncr:NCU00552]
Other DBs
ExplorEnz - The Enzyme Database: 1.3.99.30
IUBMB Enzyme Nomenclature: 1.3.99.30
ExPASy - ENZYME nomenclature database: 1.3.99.30
BRENDA, the Enzyme Database: 1.3.99.30

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