KEGG   ENZYME: 1.3.99.5
Entry
EC 1.3.99.5                 Enzyme                                 
Name
3-oxo-5alpha-steroid 4-dehydrogenase (acceptor);
steroid 5alpha-reductase;
3-oxosteroid Delta4-dehydrogenase;
3-oxo-5alpha-steroid Delta4-dehydrogenase;
steroid Delta4-5alpha-reductase;
Delta4-3-keto steroid 5alpha-reductase;
Delta4-3-oxo steroid reductase;
Delta4-3-ketosteroid5alpha-oxidoreductase;
Delta4-3-oxosteroid-5alpha-reductase;
3-keto-Delta4-steroid-5alpha-reductase;
5alpha-reductase;
testosterone 5alpha-reductase;
4-ene-3-ketosteroid-5alpha-oxidoreductase;
Delta4-5alpha-dehydrogenase;
3-oxo-5alpha-steroid:(acceptor) Delta4-oxidoreductase;
tesI (gene name)
Class
Oxidoreductases;
Acting on the CH-CH group of donors;
With unknown physiological acceptors
Sysname
3-oxo-5alpha-steroid:acceptor Delta4-oxidoreductase
Reaction(IUBMB)
a 3-oxo-5alpha-steroid + acceptor = a 3-oxo-Delta4-steroid + reduced acceptor [RN:R02643]
Reaction(KEGG)
R02643 > R01834 R04827
Substrate
3-oxo-5alpha-steroid [CPD:C02940];
acceptor [CPD:C00028]
Product
3-oxo-Delta4-steroid [CPD:C00619];
reduced acceptor [CPD:C00030]
Comment
A flavoprotein. This bacterial enzyme, characterized from Comamonas testosteroni, is involved in androsterone degradation. cf. EC 1.3.1.22, 3-oxo-5alpha-steroid 4-dehydrogenase (NADP+).
History
EC 1.3.99.5 created 1965, modified 2012
Pathway
ec00984  Steroid degradation
ec01120  Microbial metabolism in diverse environments
Orthology
K16051  3-oxo-5alpha-steroid 4-dehydrogenase
Genes
RGLCS053_01900
PREPCA10_43670
PFUWKF707C_11860
PPUTL483_14100
PPUNPP4_27190
PPUUPputUW4_01680
PKCPKB_4321
PFZAV641_10570
PLQAA042_20375
PPSYAOC04_07870
PSETTHL1_4409
PSILPMA3_11575
PKEDLD99_14665
PUMHGP31_20980
PPSHG5J76_13525
PLALFXN65_21135
PAZEKSS91_19195
PTAENCTC10697_02064(ifcA_1)
PCAVD3880_17460
CBERB5D82_11210
PHAPSHAa2146
PTNPTRA_a2599
PNGPNIG_a2794
SNANI6N98_14785
HJABST95_03540
HALCEY643_04225 EY643_18950
AFUSEYZ66_02430 EYZ66_13095
KAKKalk_07950
MSECLN244_04835
ENWMJO57_21290
SDFACG33_03810
SOKD0B54_05270 D0B54_08595 D0B54_16855
SINIGT972_08465 GT972_09470
FCEJN531_005455
GPBHDN1F_19870(tesI)
RINACS15_4365
REHH16_B0641(h16_B0641)
CNCCNE_2c05860 CNE_BB1p04270(ifcA1) CNE_BB1p06940(fccA)
CUHBJN34_30935
CTIRALTA_B0490
CBWRR42_s2342
COXE0W60_08150
BCENDM39_3421
BCEOI35_5434
BCEDDM42_6385
BCEPAPZ15_31415
BPYRABD05_28050
BCONNL30_04390
BDFWI26_20960
BTEIWS51_05515
BPSLWS57_06230
BMECWJ16_26540
BSTLBBJ41_21090
BANNJFN94_25425
BURKDM992_32830
BFNOI25_4613
CABASBC2_48770
CTTCtCNB1_1358
CTESO987_07765
DIHG7047_09730
DOEDENOEST_0485 DENOEST_3316 DENOEST_3670 DENOEST_3673
THUAC731_008850
GLOGlov_1298
NTDEGO55_03145 EGO55_05895 EGO55_13615 EGO55_13660
TSVDSM104635_00611
NARSaro_3488 Saro_3686 Saro_3721
NPPPP1Y_AT13465 PP1Y_Mpl3136 PP1Y_Mpl3259
NOTC7W88_20740
SWISwit_1005 Swit_1860 Swit_3335 Swit_3339
SPHDHY78_08855 HY78_08875 HY78_16270 HY78_21490
RDICMV14_19635
SPHMG432_01970 G432_02370 G432_07425
SSANNX02_12695 NX02_12730
SPKCKC8_03545 KC8_05240 KC8_10970
SPHAD3Y57_03785 D3Y57_15195
SLANGV829_01840
SPMIK663_02990
SPHBEP837_01509(tesI)
SPHGAZE99_00535
SFLASPHFLASMR4Y_02380(ifcA)
PHZCHX26_05335
DSYDSY0764 DSY3803
DHDDhaf_0773
DDHDesde_0646
MTVRVBD_1817
MRAMRA_1829
MTFTBFG_11847
MTBTBMG_02177
MTKTBSG_02188
MTZTBXG_002159
MTIMRGA423_11360
MTECCDC5079_1674
MTURCFBS_1909
MTLCCDC5180_1654
MTOMTCTRI2_1849
MTDUDA_1817
MTNERDMAN_2007
MTJJ112_09705
MTUBMT7199_1843
MTUCJ113_12610
MTUEJ114_09700
MTXM943_09450
MTUHI917_12910
MTULTBHG_01774
MTUTHKBT1_1906
MTUUHKBT2_1914
MTQHKBS1_1910
MBOBQ2027_MB1848
MBBBCG_1852
MBTJTY_1836
MBMBCGMEX_1833
MBKK60_019030
MAFMAF_18390
MMICRN08_2021
MCEMCAN_18331
MCQBN44_40077
MCVBN43_30955
MCXBN42_30078
MCZBN45_50080
MPAMAP_1447c MAP_1530
MAOMAP4_2309 MAP4_2397
MAVIRC58_11475 RC58_11915
MAVURE97_11485 RE97_11925
MAVMAV_2897 MAV_3022
MITOCO_28580
MIAOCU_28450
MIDMIP_03716
MYOOEM_27040
MCHIAN480_14940
MIROCQ_25850 OCQ_27160
MMALCKJ54_12985
MLPMLM_2334 MLM_2414
MMANMMAN_10300
MSAMycsm_03249 Mycsm_04160
MULMUL_3063 MUL_4900
MMCMmcs_0302 Mmcs_0316
MKMMkms_0312 Mkms_0326
MJLMjls_0293 Mjls_0307
MMIMMAR_0205 MMAR_2694
MMAEMMARE11_01890 MMARE11_26150
MMMW7S_13850
MLIMULP_00179 MULP_02440
MKNMKAN_15315
MYVG155_02520 G155_04245
MYEAB431_15170 AB431_15230 AB431_17075
MGOAFA91_21605
MHADB586_11860
MDXBTO20_15410
MSHGMSG_02470 MSG_02584
MFJMFLOJ_06530 MFLOJ_07950 MFLOJ_21820
MSTOMSTO_07790 MSTO_09190 MSTO_25520
MSIMMSIM_53030
MSAKMSAS_06930
MKUI2456_01305 I2456_01760 I2456_13340
MGROFZ046_19800
MXEMYXE_06650 MYXE_25900
MNVMNVI_11580 MNVI_40650
MPAGC0J29_01190 C0J29_15540
MNMMNVM_19340 MNVM_40340 MNVM_41400
MGORH0P51_01160 H0P51_14660 H0P51_17860
MCOOMCOO_08830 MCOO_26130
MBAIMB901379_00137 MB901379_02490(fccA)
MSEOMSEO_12700 MSEO_14010
MPSEMPSD_27690 MPSD_53970
MSHOMSHO_12420 MSHO_40180
MHEKJMUB5695_00574 JMUB5695_02538
MGAUMGALJ_22860
MLJMLAC_17620 MLAC_19640
MBRDMBRA_21110 MBRA_39620
MSHJMSHI_01900 MSHI_13780
MKRMKOR_07390 MKOR_08240
MDFK0O62_10315 K0O62_15920
MMAMK3U93_11790 K3U93_12395
MHOLK3U96_09630
MHERK3U94_10960 K3U94_11545
MPAEK0O64_14650 K0O64_14695 K0O64_16250
MSPGF6B93_00910 F6B93_11130
MOTLTS72_10745 LTS72_24685
MVMMJO54_11545
MPAAMKK62_02905
MSMMSMEG_2873
MSGMSMEI_2799 MSMEI_3570
MSBLJ00_14290 LJ00_18180
MSNLI99_14295 LI99_18185
MSHLI98_14300 LI98_18190
MVAMvan_2890 Mvan_3165
MGIMflv_3411 Mflv_4550 Mflv_4564
MSPMspyr1_27430 Mspyr1_39500 Mspyr1_39640
MCBMycch_1589 Mycch_2743
MNED174_15875
MYNMyAD_15600
MFTXA26_04570 XA26_08060
MPHLMPHLCCUG_02346(ifcA) MPHLCCUG_02608
MVQMYVA_1640 MYVA_2914 MYVA_5433
MLLB1R94_12245 B1R94_13935 B1R94_20090
MRHMycrhN_4258 MycrhN_4805
MTHN4412656_00946(ifcA_1) 4412656_02236(ifcA_2)
MHASMHAS_01796(fccA) MHAS_02476
MDUMDUV_13340 MDUV_26670
MCHTMCHIJ_12690 MCHIJ_37430
MAUUNCTC10437_02738(ifcA_1) NCTC10437_03040(ifcA_2)
MMAGMMAD_30650
MMORMMOR_26790 MMOR_32850
MFXMFAL_10920 MFAL_17780
MAICMAIC_26780 MAIC_26920 MAIC_29760
MIJMINS_21440 MINS_28360
MALVMALV_05930 MALV_29240 MALV_32080
MTYMTOK_49090
MPSCMPSYJ_36770
MARZMARA_10100
MGADMGAD_01090 MGAD_07420
MHEVMHEL_50110 MHEL_50210 MHEL_53560
MSARMSAR_38820 MSAR_41360
MANYMANY_09470 MANY_15450
MAUBMAUB_04580 MAUB_07440 MAUB_15650 MAUB_54780
MPOFMPOR_33230
MPHUMPHO_08050 MPHO_14380 MPHO_55420
MMUCC1S78_011090 C1S78_014220 C1S78_015715 C1S78_019255 C1S78_019905
MBOKMBOE_16350 MBOE_20100
MFGK6L26_27690
MFLVNCTC10271_02169(ifcA_1) NCTC10271_02186(ifcA_2)
MCEEMCEL_06110
MABMAB_0623
MMVMYCMA_0323 MYCMA_1290
MABBMASS_0593 MASS_2307
MABLMMASJCM_0612 MMASJCM_2330
MCHEBB28_02985 BB28_11950
MIZBAB75_03735 BAB75_12875
MSTEMSTE_00582 MSTE_02324
MSAOMYCSP_02685 MYCSP_11035
MSALDSM43276_00541 DSM43276_02107
MJDJDM601_1914 JDM601_2019 JDM601_4251
MTER4434518_01865(ifcA) 4434518_01971
MMINMMIN_35580 MMIN_36500
MHIBMHIB_10920 MHIB_13900 MHIB_14890
MPAKMIU77_08035 MIU77_08480
NFANFA_30830 NFA_4640 NFA_46590
NFRERS450000_00982(ifcA_1) ERS450000_03773(ifcA_3) ERS450000_05269(ifcA_4)
NCYNOCYR_0491 NOCYR_3994 NOCYR_4393
NBRO3I_002615 O3I_020135 O3I_020920
NNONONO_c03420
NSLBOX37_16630 BOX37_17180
NSRNS506_00426(tesI) NS506_06811
NTPCRH09_02420
NOZDMB37_00255
NODFOH10_02275 FOH10_22680 FOH10_29845
NAHF5544_02605
NADNCTC11293_03870(ifcA_1) NCTC11293_04109(ifcA_2)
NWLNWFMUON74_03310
NIEKV110_02995 KV110_20565
NTCKHQ06_07725 KHQ06_35220 KHQ06_36955
NHUH0264_02930 H0264_34360 H0264_36175
NYALTV02_29815 LTV02_37155 LTV02_38585
NGPLTT66_15370 LTT66_28710 LTT66_35130
RHARHA1_ro05698
RERRER_07380 RER_33860 RER_43230
REYO5Y_03585 O5Y_15605 O5Y_20260
REBXU06_03825 XU06_15840 XU06_20430
RQIC1M55_04070 C1M55_16855 C1M55_21775
ROPROP_57660
ROAPd630_LPD02312
REQREQ_07930 REQ_08850 REQ_12430 REQ_13730
RPYY013_20155
RBYCEJ39_06655
RAVAAT18_05750 AAT18_07275
RHWBFN03_01840
RRZCS378_20980 CS378_22345
RHQIM25_09985
RHODAOT96_01820 AOT96_14465 AOT96_27040
RRT4535765_00633(ifcA_1) 4535765_00838(ifcA_2)
RCRNCTC10994_01879(ifcA_1) NCTC10994_02087(ifcA_2) NCTC10994_03292(ifcA_5)
RTMG4H71_04820 G4H71_07430
RKOJWS14_31210
RGOKYT97_05695 KYT97_15530 KYT97_16405 KYT97_23105 KYT97_28850
GORKTR9_4518
GOQACH46_10190 ACH46_19775
GTABCM27_04400 BCM27_23650
GOCCXX93_03785
GITC6V83_00900
GRUGCWB2_04130(ifcA1) GCWB2_22595(ifcA3)
GOMD7316_02580(urdA) D7316_03032(ifcA)
GAVC5O27_05830
GODGKZ92_21595
GAMGII34_01515
SPINKV203_10735
TPRTpau_3846
TSMASU32_08060 ASU32_21910
DTMBJL86_0019
DITC3V38_06510
DIZCT688_00070
DPCA6048_00070
DLUA6035_00120
CBQAL705_03940
TOYFO059_01500 FO059_06620 FO059_16680
SALUDC74_8142
SALLSAZ_42040
SNKCP967_03275
SXNIAG42_07600
AARCG127AT_04070
ARLAFL94_08135
ARYATC04_09765
AJGKKR91_06375
ASUNKG104_04150
GLUF0M17_08150
GPRJQN66_08300
NCANoca_0296
NDKI601_0577(urdA) I601_1513(ifcA_1)
NBEBack2_26880
NANOG5V58_21245
NAROCFH99_22010
NPIG7071_12495
PSIMKR76_13710 KR76_24505
AEBC6I20_03515
TCUTcur_3515
ACTWF7P10_05400 F7P10_28365
FALFRAAL3038
AMDAMED_2521
AMNRAM_12815
AMMAMES_2493
AMZB737_2494
AOIAORI_2483
AJAAJAP_26410
AMQAMETH_4839
AMYCCU254_10455 CU254_23445
AMYBBKN51_05015
AABA4R43_16050
AMYYYIM_14445 YIM_36750(urdA)
AORISD37_13480
AACDLWP59_23765 LWP59_29205
KALKALB_5139
KPHYAOZ06_42835
LEDBBK82_38945
ALOCRK59602
PMADBAY61_10950
SACEGIY23_13360
STPStrop_2675
SAQSare_2883
AMSAMIS_19130
AIHAiant_18970
ABAIIMCC26256_11535
EGDGS424_005060 GS424_006825
AHATADCFC_00230 ADCFC_06820 ADCFC_14480
RTSCE91St31_06850
 » show all
Reference
1  [PMID:13673006]
  Authors
LEVY HR, TALALAY P.
  Title
Bacterial oxidation of steroids. II. Studies on the enzymatic mechanism of ring A dehydrogenation.
  Journal
J Biol Chem 234:2014-21 (1959)
Reference
2  [PMID:8655514]
  Authors
Florin C, Kohler T, Grandguillot M, Plesiat P
  Title
Comamonas testosteroni 3-ketosteroid-delta 4(5 alpha)-dehydrogenase: gene and protein characterization.
  Journal
J Bacteriol 178:3322-30 (1996)
DOI:10.1128/JB.178.11.3322-3330.1996
  Sequence
Reference
3  [PMID:12902225]
  Authors
Horinouchi M, Hayashi T, Yamamoto T, Kudo T
  Title
A new bacterial steroid degradation gene cluster in Comamonas testosteroni TA441 which consists of aromatic-compound degradation genes for seco-steroids and 3-ketosteroid dehydrogenase genes.
  Journal
Appl Environ Microbiol 69:4421-30 (2003)
DOI:10.1128/AEM.69.8.4421-4430.2003
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 1.3.99.5
IUBMB Enzyme Nomenclature: 1.3.99.5
ExPASy - ENZYME nomenclature database: 1.3.99.5
BRENDA, the Enzyme Database: 1.3.99.5
CAS: 9036-43-5

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