KEGG   ENZYME: 1.3.99.8Help
Entry
EC 1.3.99.8                 Enzyme                                 

Name
2-furoyl-CoA dehydrogenase;
furoyl-CoA hydroxylase;
2-furoyl coenzyme A hydroxylase;
2-furoyl coenzyme A dehydrogenase;
2-furoyl-CoA:(acceptor) 5-oxidoreductase (hydroxylating)
Class
Oxidoreductases;
Acting on the CH-CH group of donors;
With unknown physiological acceptors
BRITE hierarchy
Sysname
2-furoyl-CoA:acceptor 5-oxidoreductase (hydroxylating)
Reaction(IUBMB)
2-furoyl-CoA + H2O + acceptor = S-(5-hydroxy-2-furoyl)-CoA + reduced acceptor [RN:R02987]
Reaction(KEGG)
Substrate
2-furoyl-CoA [CPD:C00845];
H2O [CPD:C00001];
acceptor [CPD:C00028]
Product
S-(5-hydroxy-2-furoyl)-CoA [CPD:C03724];
reduced acceptor [CPD:C00030]
Comment
A copper protein. The oxygen atom of the -OH produced is derived from water, not O2; the actual oxidative step is probably dehydrogenation of a hydrated form -CHOH-CH2- to -C(OH)=CH-, which tautomerizes non-enzymically to -CO-CH2-, giving (5-oxo-4,5-dihydro-2-furoyl)-CoA. Methylene blue, nitro blue, tetrazolium and a membrane fraction from Pseudomonas putida can act as acceptors.
History
EC 1.3.99.8 created 1976
Pathway
ec00365  Furfural degradation
ec01120  Microbial metabolism in diverse environments
Orthology
K16877  2-furoyl-CoA dehydrogenase large subunit
K16878  2-furoyl-CoA dehydrogenase FAD binding subunit
K16879  2-furoyl-CoA dehydrogenase 2Fe-2S iron sulfur subunit
Genes
PPSE: BN5_2298(hmfA) BN5_2299(hmfB) BN5_2300(hmfC)
PMAN: OU5_0887 OU5_0888 OU5_0889
PSTT: CH92_11530 CH92_11535 CH92_11540
PPUU: PputUW4_02623 PputUW4_02624 PputUW4_02625
PSIL: PMA3_09770 PMA3_09780
ADI: B5T_02845 B5T_02846 B5T_02847
RPI: Rpic_2052 Rpic_2053 Rpic_2054
RPF: Rpic12D_1744 Rpic12D_1745 Rpic12D_1746
RPJ: N234_37060 N234_37065 N234_37070
REU: Reut_C5888 Reut_C5889 Reut_C5890
REH: H16_A1701(coxS3) H16_A1702(h16_A1702) H16_A1703(coxL3)
BJA: blr3533(cutL) blr3534(cutM) blr3535
MAQU: Maq22A_c13995(coxL)
DSH: Dshi_3719(coxL) Dshi_3720(coxM) Dshi_3721(coxS)
AZL: AZL_a11000 AZL_a11010(cutM) AZL_a11020(xdhB)
ANM: GFC28_297
PDX: Psed_5933
PSEA: WY02_08310
STO: STK_00930
PYR: P186_1143
POG: Pogu_1990
 » show all
Taxonomy
Reference
1  [PMID:4655411]
  Authors
Kitcher JP, Trudgill PW, Rees JS.
  Title
Purification and properties of 2-furoyl-coenzyme A hydroxylase from Pseudomonas putida F2.
  Journal
Biochem J 130:121-32 (1972)
Other DBs
ExplorEnz - The Enzyme Database: 1.3.99.8
IUBMB Enzyme Nomenclature: 1.3.99.8
ExPASy - ENZYME nomenclature database: 1.3.99.8
UM-BBD (Biocatalysis/Biodegradation Database): 1.3.99.8
BRENDA, the Enzyme Database: 1.3.99.8
CAS: 9068-18-2

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