KEGG   ENZYME: 1.5.1.28Help
Entry
EC 1.5.1.28                 Enzyme                                 

Name
opine dehydrogenase;
(2S)-2-{[1-(R)-carboxyethyl]amino}pentanoate dehydrogenase (NAD+, L-aminopentanoate-forming)
Class
Oxidoreductases;
Acting on the CH-NH group of donors;
With NAD+ or NADP+ as acceptor
BRITE hierarchy
Sysname
(2S)-2-{[1-(R)-carboxyethyl]amino}pentanoate:NAD+ oxidoreductase (L-aminopentanoate-forming)
Reaction(IUBMB)
(2S)-2-{[1-(R)-carboxyethyl]amino}pentanoate + NAD+ + H2O = L-2-aminopentanoic acid + pyruvate + NADH + H+ [RN:R03732]
Reaction(KEGG)
Substrate
(2S)-2-{[1-(R)-carboxyethyl]amino}pentanoate [CPD:C06326];
NAD+ [CPD:C00003];
H2O [CPD:C00001]
Product
L-2-aminopentanoic acid [CPD:C01826];
pyruvate [CPD:C00022];
NADH [CPD:C00004];
H+ [CPD:C00080]
Comment
In the forward direction, the enzyme from Arthrobacter sp. acts also on secondary amine dicarboxylates such as N-(1-carboxyethyl)methionine and N-(1-carboxyethyl)phenylalanine. Dehydrogenation forms an imine, which dissociates to the amino acid and pyruvate. In the reverse direction, the enzyme acts also on neutral amino acids as an amino donor. They include L-amino acids such as 2-aminopentanoic acid, 2-aminobutyric acid, 2-aminohexanoic acid, 3-chloroalanine, O-acetylserine, methionine, isoleucine, valine, phenylalanine, leucine and alanine. The amino acceptors include 2-oxoacids such as pyruvate, oxaloacetate, glyoxylate and 2-oxobutyrate.
History
EC 1.5.1.28 created 1999
Orthology
K04940  opine dehydrogenase
Genes
KPY: KPNIH31_18350
DDA: Dd703_1794
PSTA: BGK56_13885
VSC: VSVS12_01102
PPR: PBPRB0771
PGB: H744_2c1740
PDS: CAY62_19020
PPU: PP_4452
PPJ: RK21_02019
PSW: LK03_15960
LLO: LLO_2372
BTQ: BTQ_3630
BTJ: BTJ_4666
BTZ: BTL_5454
BTV: BTHA_4749
BTHE: BTN_5124
BTHM: BTRA_5393
BTHA: DR62_5254
BTHL: BG87_5460
BOK: DM82_6318
BOC: BG90_4356
BCN: Bcen_4737
BCJ: BCAM0655
BCEN: DM39_4502
BCEO: I35_4551
BAM: Bamb_5362
BMU: Bmul_5067
BMK: DM80_4242
BMUL: NP80_3656
BCED: DM42_4435
BCON: NL30_22575
BTEI: WS51_01775
BSEM: WJ12_23245
BPSL: WS57_10650
BMEC: WJ16_22265
AXX: ERS451415_04062(odh)
AFQ: AFA_01515
LIM: L103DPR2_02654(odh)
GLO: Glov_2392
DRT: Dret_0075
MES: Meso_2393
AMIH: CO731_00337(odh_1) CO731_05356(odh_2) CO731_05401(odh_3)
SMER: DU99_31935
SHZ: shn_28825
BJA: blr6086
BRA: BRADO2316
BBT: BBta_2679
BRS: S23_21890
BRAD: BF49_5547
SIL: SPO1466
RDE: RD1_0281
AHT: ANTHELSMS3_01768(odh)
SWI: Swit_0736
SPHD: HY78_23015
BCER: BCK_09040
AXL: AXY_19850
SAU: SA2095
SAV: SAV2302
SAW: SAHV_2286
SAM: MW2220
SAS: SAS2192
SAR: SAR2386
SAC: SACOL2293
SAE: NWMN_2201
SAD: SAAV_2363
SUZ: MS7_2319
SUG: SAPIG2352
SAUA: SAAG_00127
SAUS: SA40_2047
SAUU: SA957_2131
SAUG: SA268_2202
SAUF: X998_2283
SAB: SAB2174c
SAUB: C248_2341
SAUC: CA347_2380
SAUR: SABB_01371
SAUI: AZ30_12130
SAUD: CH52_07465
SAMS: NI36_11355
SEP: SE1874
SER: SERP1882
SEPP: SEB_01886
SEPS: DP17_824
SHA: SH0757
SSP: SSP0611
SCA: SCA_1793
SDT: SPSE_0521
SPAS: STP1_0780
SCAP: AYP1020_1476(odh)
SSCH: LH95_02730
SSCZ: RN70_03005
SAGQ: EP23_07195
PSAB: PSAB_03580
LFF: LBFF_0980
LRG: LRHM_0461
JDA: BW727_101397(odh_1) BW727_101917(odh_2)
AMT: Amet_2956
HSD: SD1D_0903
STH: STH257
ELM: ELI_3684
AWO: Awo_c27890(odh)
TMR: Tmar_0905
TTE: TTE0923
THX: Thet_0827
TIT: Thit_0861
TKI: TKV_c09360(odh)
TAE: TepiRe1_0401(odh)
TOC: Toce_0231
TTM: Tthe_0328
TSH: Tsac_0905
AIN: Acin_2030
ERH: ERH_0138
LPIL: LIP_3237
NFA: NFA_20460
NFR: ERS450000_02063(odh)
GOR: KTR9_4000
BCV: Bcav_1678
MPH: MLP_28670(odh)
RCA: Rcas_2335
ATM: ANT_01050
TLE: Tlet_0900
TME: Tmel_0083
TAF: THA_300
PMO: Pmob_0984
DTN: DTL3_0117
KOL: Kole_0924
LOKI: Lokiarch_08200(odh)
 » show all
Taxonomy
Reference
1  [PMID:2753861]
  Authors
Asano Y, Yamaguchi K, Kondo K.
  Title
A new NAD+-dependent opine dehydrogenase from Arthrobacter sp. strain 1C.
  Journal
J Bacteriol 171:4466-71 (1989)
DOI:10.1128/JB.171.8.4466-4471.1989
Reference
2  [PMID:7487048]
  Authors
Dairi T, Asano Y.
  Title
Cloning, nucleotide sequencing, and expression of an opine dehydrogenase gene from Arthrobacter sp. strain 1C.
  Journal
Appl Environ Microbiol 61:3169-71 (1995)
  Sequence
Reference
3
  Authors
Kato, Y., Yamada, H. and Asano, Y.
  Title
Stereoselective synthesis of opine-type secondary amine carboxylic acids by a new enzyme opine dehydrogenase. Use of recombinant enzymes.
  Journal
J Mol Catal, B Enzym 1:151-160 (1996)
Other DBs
ExplorEnz - The Enzyme Database: 1.5.1.28
IUBMB Enzyme Nomenclature: 1.5.1.28
ExPASy - ENZYME nomenclature database: 1.5.1.28
BRENDA, the Enzyme Database: 1.5.1.28
CAS: 108281-02-3

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