KEGG   ENZYME: 1.5.1.43Help
Entry
EC 1.5.1.43                 Enzyme                                 

Name
carboxynorspermidine synthase;
carboxynorspermidine dehydrogenase;
carboxyspermidine dehydrogenase;
CASDH;
CANSDH;
VC1624 (gene name)
Class
Oxidoreductases;
Acting on the CH-NH group of donors;
With NAD+ or NADP+ as acceptor
BRITE hierarchy
Sysname
carboxynorspermidine:NADP+ oxidoreductase
Reaction(IUBMB)
(1) carboxynorspermidine + H2O + NADP+ = L-aspartate 4-semialdehyde + propane-1,3-diamine + NADPH + H+ [RN:R09080];
(2) carboxyspermidine + H2O + NADP+ = L-aspartate 4-semialdehyde + putrescine + NADPH + H+ [RN:R09079]
Reaction(KEGG)
Substrate
carboxynorspermidine [CPD:C18174];
H2O [CPD:C00001];
NADP+ [CPD:C00006];
carboxyspermidine [CPD:C18172]
Product
L-aspartate 4-semialdehyde [CPD:C00441];
propane-1,3-diamine [CPD:C00986];
NADPH [CPD:C00005];
H+ [CPD:C00080];
putrescine [CPD:C00134]
Comment
The reaction takes place in the opposite direction. Part of a bacterial polyamine biosynthesis pathway. L-aspartate 4-semialdehyde and propane-1,3-diamine/putrescine form a Schiff base that is reduced to form carboxynorspermidine/carboxyspermidine, respectively [1]. The enzyme from the bacterium Vibrio cholerae is essential for biofilm formation [2]. The enzyme from Campylobacter jejuni only produces carboxyspermidine in vivo even though it also can produce carboxynorspermidine in vitro [3].
History
EC 1.5.1.43 created 2012
Pathway
ec00330  Arginine and proline metabolism
ec01100  Metabolic pathways
Orthology
K13746  carboxynorspermidine synthase
Genes
VCH: VC1624
VCF: IR04_13345
VCS: MS6_1406
VCE: Vch1786_I1124
VCQ: EN18_11315
VCJ: VCD_002750
VCI: O3Y_07890
VCO: VC0395_A1231
VCR: VC395_1742
VCM: VCM66_1564
VVU: VV1_3049
VVY: VV1237
VPA: VP1941
VPB: VPBB_1783
VAG: N646_1023
VSP: VS_1946
VAN: VAA_01989
VTA: A0392
PPR: PBPRA2229(RB1550)
PIN: Ping_2289
MVS: MVIS_2445
 » show all
Taxonomy
Reference
1  [PMID:1955861]
  Authors
Nakao H, Shinoda S, Yamamoto S
  Title
Purification and some properties of carboxynorspermidine synthase participating in a novel biosynthetic pathway for norspermidine in Vibrio alginolyticus.
  Journal
J Gen Microbiol 137:1737-42 (1991)
DOI:10.1099/00221287-137-7-1737
Reference
2  [PMID:19196710]
  Authors
Lee J, Sperandio V, Frantz DE, Longgood J, Camilli A, Phillips MA, Michael AJ.
  Title
An alternative polyamine biosynthetic pathway is widespread in bacteria and essential for biofilm formation in Vibrio cholerae.
  Journal
J Biol Chem 284:9899-907 (2009)
DOI:10.1074/jbc.M900110200
  Sequence
[vch:VC1624]
Reference
3  [PMID:22025614]
  Authors
Hanfrey CC, Pearson BM, Hazeldine S, Lee J, Gaskin DJ, Woster PM, Phillips MA, Michael AJ
  Title
Alternative spermidine biosynthetic route is critical for growth of Campylobacter jejuni and is the dominant polyamine pathway in human gut microbiota.
  Journal
J Biol Chem 286:43301-12 (2011)
DOI:10.1074/jbc.M111.307835
Other DBs
ExplorEnz - The Enzyme Database: 1.5.1.43
IUBMB Enzyme Nomenclature: 1.5.1.43
ExPASy - ENZYME nomenclature database: 1.5.1.43
BRENDA, the Enzyme Database: 1.5.1.43

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