KEGG   ENZYME: 1.5.99.14Help
Entry
EC 1.5.99.14                Enzyme                                 

Name
6-hydroxypseudooxynicotine dehydrogenase
Class
Oxidoreductases;
Acting on the CH-NH group of donors;
With unknown physiological acceptors
BRITE hierarchy
Sysname
1-(6-hydroxypyridin-3-yl)-4-(methylamino)butan-1-one:acceptor 6-oxidoreductase (hydroxylating)
Reaction(IUBMB)
1-(6-hydroxypyridin-3-yl)-4-(methylamino)butan-1-one + acceptor + H2O = 1-(2,6-dihydroxypyridin-3-yl)-4-(methylamino)butan-1-one + reduced acceptor [RN:R07514]
Reaction(KEGG)
Substrate
1-(6-hydroxypyridin-3-yl)-4-(methylamino)butan-1-one [CPD:C01297];
acceptor [CPD:C00028];
H2O [CPD:C00001]
Product
1-(2,6-dihydroxypyridin-3-yl)-4-(methylamino)butan-1-one [CPD:C15986];
reduced acceptor [CPD:C00030]
Comment
Contains a cytidylyl molybdenum cofactor [3]. The enzyme, which participates in the nicotine degradation pathway, has been characterized from the soil bacterium Arthrobacter nicotinovorans [1,2].
History
EC 1.5.99.14 created 2012
Pathway
ec00760  Nicotinate and nicotinamide metabolism
ec01120  Microbial metabolism in diverse environments
Orthology
K19185  6-hydroxypseudooxynicotine dehydrogenase subunit alpha
K19186  6-hydroxypseudooxynicotine dehydrogenase subunit beta
K19187  6-hydroxypseudooxynicotine dehydrogenase subunit gamma
Genes
ROP: ROP_27460(kdhL) ROP_27510(kdhM) ROP_27520(kdhB)
ROA: Pd630_LPD07471 Pd630_LPD07476 Pd630_LPD07477
NCA: Noca_0606 Noca_0607 Noca_0612
Taxonomy
Reference
1
  Authors
Freudenberg, W., Konig, K. and Andreesen, J. R.
  Title
Nicotine dehydrogenase from Arthrobacter oxidans: A molybdenum-containing hydroxylase.
  Journal
FEMS Microbiology Letters 52:13-18 (1988)
Reference
2  [PMID:7815950]
  Authors
Grether-Beck S, Igloi GL, Pust S, Schilz E, Decker K, Brandsch R
  Title
Structural analysis and molybdenum-dependent expression of the pAO1-encoded nicotine dehydrogenase genes of Arthrobacter nicotinovorans.
  Journal
Mol Microbiol 13:929-36 (1994)
DOI:10.1111/j.1365-2958.1994.tb00484.x
Reference
3  [PMID:16820521]
  Authors
Sachelaru P, Schiltz E, Brandsch R
  Title
A functional mobA gene for molybdopterin cytosine dinucleotide cofactor biosynthesis is required for activity and holoenzyme assembly of the heterotrimeric nicotine dehydrogenases of Arthrobacter nicotinovorans.
  Journal
Appl Environ Microbiol 72:5126-31 (2006)
DOI:10.1128/AEM.00437-06
Other DBs
ExplorEnz - The Enzyme Database: 1.5.99.14
IUBMB Enzyme Nomenclature: 1.5.99.14
ExPASy - ENZYME nomenclature database: 1.5.99.14
UM-BBD (Biocatalysis/Biodegradation Database): 1.5.99.14
BRENDA, the Enzyme Database: 1.5.99.14

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