KEGG   ENZYME: 1.7.1.14Help
Entry
EC 1.7.1.14                 Enzyme                                 

Name
nitric oxide reductase [NAD(P)+, nitrous oxide-forming];
fungal nitric oxide reductase;
cytochrome P450nor;
NOR (ambiguous)
Class
Oxidoreductases;
Acting on other nitrogenous compounds as donors;
With NAD+ or NADP+ as acceptor
BRITE hierarchy
Sysname
nitrous oxide:NAD(P) oxidoreductase
Reaction(IUBMB)
N2O + NAD(P)+ + H2O = 2 NO + NAD(P)H + H+ [RN:R02492 R09446]
Reaction(KEGG)
Substrate
N2O [CPD:C00887];
NAD+ [CPD:C00003];
NADP+ [CPD:C00006];
H2O [CPD:C00001]
Product
NO [CPD:C00533];
NADH [CPD:C00004];
NADPH [CPD:C00005];
H+ [CPD:C00080]
Comment
A heme-thiolate protein (P-450). The enzyme from Fusarium oxysporum utilizes only NADH, but the isozyme from Trichosporon cutaneum utilizes both NADH and NADPH. The electron transfer from NAD(P)H to heme occurs directly, not requiring flavin or other redox cofactors.
History
EC 1.7.1.14 created 2011
Pathway
ec00910  Nitrogen metabolism
ec01120  Microbial metabolism in diverse environments
Orthology
K15877  fungal nitric oxide reductase
Genes
CRE: CHLREDRAFT_196664(CYP55B1)
CVR: CHLNCDRAFT_51513
NCR: NCU06137
NTE: NEUTE1DRAFT117740(NEUTE1DRAFT_117740)
SMP: SMAC_06378
PAN: PODANSg1411
TTT: THITE_2117644
FGR: FGSG_11585
FVR: FVEG_10773
FOX: FOXG_12350 FOXG_17502
MBE: MBM_04680
ANG: ANI_1_2092064(An07g08930)
ABE: ARB_00546
TVE: TRV_00378
 » show all
Taxonomy
Reference
1  [PMID:2040619]
  Authors
Shoun H, Tanimoto T
  Title
Denitrification by the fungus Fusarium oxysporum and involvement of cytochrome P-450 in the respiratory nitrite reduction.
  Journal
J Biol Chem 266:11078-82 (1991)
  Sequence
Reference
2  [PMID:7829493]
  Authors
Shiro Y, Fujii M, Iizuka T, Adachi S, Tsukamoto K, Nakahara K, Shoun H
  Title
Spectroscopic and kinetic studies on reaction of cytochrome P450nor with nitric oxide. Implication for its nitric oxide reduction mechanism.
  Journal
J Biol Chem 270:1617-23 (1995)
DOI:10.1074/jbc.270.4.1617
  Sequence
Reference
3  [PMID:12105197]
  Authors
Zhang L, Kudo T, Takaya N, Shoun H
  Title
The B' helix determines cytochrome P450nor specificity for the electron donors NADH and NADPH.
  Journal
J Biol Chem 277:33842-7 (2002)
DOI:10.1074/jbc.M203923200
  Sequence
Reference
4  [PMID:15313618]
  Authors
Oshima R, Fushinobu S, Su F, Zhang L, Takaya N, Shoun H
  Title
Structural evidence for direct hydride transfer from NADH to cytochrome P450nor.
  Journal
J Mol Biol 342:207-17 (2004)
DOI:10.1016/j.jmb.2004.07.009
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 1.7.1.14
IUBMB Enzyme Nomenclature: 1.7.1.14
ExPASy - ENZYME nomenclature database: 1.7.1.14
BRENDA, the Enzyme Database: 1.7.1.14

DBGET integrated database retrieval system