Oxidoreductases;
Acting on other nitrogenous compounds as donors;
With oxygen as acceptor
BRITE hierarchy

urate:oxygen oxidoreductase

urate + O2 + H2O = 5-hydroxyisourate + H2O2 [RN:R02106]

R02106;
(other) R07981
Reaction

urate [CPD:C00366];
O2 [CPD:C00007];
H2O [CPD:C00001]

This enzyme was previously thought to be a copper protein, but it is now known that the enzymes from soy bean (Glycine max), the mould Aspergillus flavus and Bacillus subtilis contains no copper nor any other transition-metal ion. The 5-hydroxyisourate formed decomposes spontaneously to form allantoin and CO2, although there is an enzyme-catalysed pathway in which EC 3.5.2.17, hydroxyisourate hydrolase, catalyses the first step. The enzyme is different from EC 1.14.13.113 (FAD-dependent urate hydroxylase).

EC 1.7.3.3 created 1961, modified 2002, modified 2005, modified 2010

1  [PMID:13363909]

HUDSON PB, LONDON M.

Purification and properties of solubilized uricase.

Biochim Biophys Acta 21:290-8 (1956)
DOI:10.1016/0006-3002(56)90010-5

2  [PMID:13271340]

MAHLER HR, HUBSCHER G, BAUM R.

Studies on uricase.  I.  Preparation, purification, and properties of a cuproprotein.

J Biol Chem 216:625-41 (1955)

3  [PMID:13252004]

ROBBINS KC, BARNETT EL, GRANT NH.

Partial pruficiation of procine liver uricase.

J Biol Chem 216:27-35 (1955)

4  [PMID:9709003]

Kahn K, Tipton PA.

Spectroscopic characterization of intermediates in the urate oxidase reaction.

Biochemistry 37:11651-9 (1998)
DOI:10.1021/bi980446g

5  [PMID:9360612]

Colloc'h N, el Hajji M, Bachet B, L'Hermite G, Schiltz M, Prange T, Castro B, Mornon JP

Crystal structure of the protein drug urate oxidase-inhibitor complex at 2.05 A resolution.

Nat Struct Biol 4:947-52 (1997)
DOI:10.1038/nsb1197-947

[afv:AFLA_044090]

6  [PMID:12680763]

Imhoff RD, Power NP, Borrok MJ, Tipton PA.

General base catalysis in the urate oxidase reaction: evidence for a novel Thr-Lys catalytic diad.

Biochemistry 42:4094-100 (2003)
DOI:10.1021/bi027377x