| Entry |
|
|---|
| Name |
factor-independent urate hydroxylase;
uric acid oxidase;
uricase;
uricase II;
urate oxidase
|
|---|
| Class |
Oxidoreductases;
Acting on other nitrogenous compounds as donors;
With oxygen as acceptor
 |
|---|
| Sysname |
urate:oxygen oxidoreductase
|
|---|
| Reaction(IUBMB) |
urate + O2 + H2O = 5-hydroxyisourate + H2O2 [RN: R02106]
|
|---|
| Reaction(KEGG) |
|
|---|
| Substrate |
|
|---|
| Product |
|
|---|
| Comment |
This enzyme was previously thought to be a copper protein, but it is now known that the enzymes from soy bean (Glycine max), the mould Aspergillus flavus and Bacillus subtilis contains no copper nor any other transition-metal ion. The 5-hydroxyisourate formed decomposes spontaneously to form allantoin and CO2, although there is an enzyme-catalysed pathway in which EC 3.5.2.17, hydroxyisourate hydrolase, catalyses the first step. The enzyme is different from EC 1.14.13.113 (FAD-dependent urate hydroxylase).
|
|---|
| History |
EC 1.7.3.3 created 1961, modified 2002, modified 2005, modified 2010
|
|---|
| Pathway |
| ec01120 | Microbial metabolism in diverse environments |
|
|---|
| Orthology |
| K16838 | urate oxidase / 2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase |
|
|---|
| Genes |
» show all
 |
|---|
| Reference |
|
|---|
| Authors |
HUDSON PB, LONDON M. |
|---|
| Title |
Purification and properties of solubilized uricase. |
|---|
| Journal |
|
|---|
| Reference |
|
|---|
| Authors |
MAHLER HR, HUBSCHER G, BAUM R. |
|---|
| Title |
Studies on uricase. I. Preparation, purification, and properties of a cuproprotein. |
|---|
| Journal |
J Biol Chem 216:625-41 (1955) |
|---|
| Reference |
|
|---|
| Authors |
ROBBINS KC, BARNETT EL, GRANT NH. |
|---|
| Title |
Partial pruficiation of procine liver uricase. |
|---|
| Journal |
J Biol Chem 216:27-35 (1955) |
|---|
| Reference |
|
|---|
| Authors |
Kahn K, Tipton PA. |
|---|
| Title |
Spectroscopic characterization of intermediates in the urate oxidase reaction. |
|---|
| Journal |
|
|---|
| Reference |
|
|---|
| Authors |
Colloc'h N, el Hajji M, Bachet B, L'Hermite G, Schiltz M, Prange T, Castro B, Mornon JP. |
|---|
| Title |
Crystal structure of the protein drug urate oxidase-inhibitor complex at 2.05 A resolution. |
|---|
| Journal |
Nat Struct Biol 4:947-52 (1997) |
|---|
| Sequence |
|
|---|
| Reference |
|
|---|
| Authors |
Imhoff RD, Power NP, Borrok MJ, Tipton PA. |
|---|
| Title |
General base catalysis in the urate oxidase reaction: evidence for a novel Thr-Lys catalytic diad. |
|---|
| Journal |
|
|---|
| Other DBs |
| ExplorEnz - The Enzyme Database: | 1.7.3.3 |
| ExPASy - ENZYME nomenclature database: | 1.7.3.3 |
| UM-BBD (Biocatalysis/Biodegradation Database): | 1.7.3.3 |
| BRENDA, the Enzyme Database: | 1.7.3.3 |
|
|---|
