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Database: ENZYME
Entry: 2.1.1.156
LinkDB: 2.1.1.156
ENTRY       EC 2.1.1.156                Enzyme
NAME        glycine/sarcosine N-methyltransferase;
            ApGSMT;
            glycine-sarcosine methyltransferase;
            GSMT;
            GMT;
            glycine sarcosine N-methyltransferase;
            S-adenosyl-L-methionine:sarcosine N-methyltransferase
CLASS       Transferases;
            Transferring one-carbon groups;
            Methyltransferases
SYSNAME     S-adenosyl-L-methionine:glycine(or sarcosine) N-methyltransferase [sarcosine(or N,N-dimethylglycine)-forming]
REACTION    2 S-adenosyl-L-methionine + glycine = 2 S-adenosyl-L-homocysteine + N,N-dimethylglycine (overall reaction) [RN:R10060];
            (1a) S-adenosyl-L-methionine + glycine = S-adenosyl-L-homocysteine + sarcosine [RN:R00367];
            (1b) S-adenosyl-L-methionine + sarcosine = S-adenosyl-L-homocysteine + N,N-dimethylglycine [RN:R07243]
ALL_REAC    R00367 R07243 R10060
SUBSTRATE   S-adenosyl-L-methionine [CPD:C00019];
            glycine [CPD:C00037];
            sarcosine [CPD:C00213]
PRODUCT     S-adenosyl-L-homocysteine [CPD:C00021];
            N,N-dimethylglycine [CPD:C01026];
            sarcosine [CPD:C00213]
COMMENT     Cells of the oxygen-evolving halotolerant cyanobacterium Aphanocthece halophytica synthesize betaine from glycine by a three-step methylation process. This is the first enzyme and it leads to the formation of either sarcosine or N,N-dimethylglycine, which is further methylated to yield betaine (N,N,N-trimethylglycine) by the action of EC 2.1.1.157, sarcosine/dimethylglycine N-methyltransferase. Differs from EC 2.1.1.20, glycine N-methyltransferase, as it can further methylate the product of the first reaction. Acetate, dimethylglycine and S-adenosyl-L-homocysteine can inhibit the reaction [3].
HISTORY     EC 2.1.1.156 created 2005
REFERENCE   1  [PMID:10896953]
  AUTHORS   Nyyssola A, Kerovuo J, Kaukinen P, von Weymarn N, Reinikainen T.
  TITLE     Extreme halophiles synthesize betaine from glycine by methylation.
  JOURNAL   J Biol Chem 275:22196-201 (2000)
            DOI:10.1074/jbc.M910111199
  SEQUENCE  [ag:AAF87202 AAF87204]
REFERENCE   2  [PMID:11319079]
  AUTHORS   Nyyssola A, Reinikainen T, Leisola M.
  TITLE     Characterization of glycine sarcosine N-methyltransferase and sarcosine dimethylglycine N-methyltransferase.
  JOURNAL   Appl Environ Microbiol 67:2044-50 (2001)
            DOI:10.1128/AEM.67.5.2044-2050.2001
  SEQUENCE  [ag:AAF87202]
REFERENCE   3  [PMID:12466265]
  AUTHORS   Waditee R, Tanaka Y, Aoki K, Hibino T, Jikuya H, Takano J, Takabe T, Takabe T.
  TITLE     Isolation and functional characterization of N-methyltransferases that catalyze betaine synthesis from glycine in a halotolerant photosynthetic organism Aphanothece halophytica.
  JOURNAL   J Biol Chem 278:4932-42 (2003)
            DOI:10.1074/jbc.M210970200
  SEQUENCE  [ag:BAC56939]
PATHWAY     ec00260  Glycine, serine and threonine metabolism
ORTHOLOGY   K18896  glycine/sarcosine N-methyltransferase
GENES       PFS: PFLU_2095
            PFW: PF1751_v1c19160
            PFF: PFLUOLIPICF727140
            PFX: A7318_08950
            PTV: AA957_04385
            PAZO: AYR47_17170
            TIG: THII_1900 THII_1901
            TMB: Thimo_1798 Thimo_2027
            MPUR: MARPU_03465
            TEE: Tel_06780
            HHA: Hhal_1068 Hhal_1678
            HHC: M911_05560 M911_09985
            TGR: Tgr7_1181
            TKM: TK90_0179
            TNI: TVNIR_0234
            TTI: THITH_16330
            TVR: TVD_00875
            EBS: ECTOBSL9_2257 ECTOBSL9_2476
            SALN: SALB1_0939
            TTC: FOKN1_2395
            HOH: Hoch_6748
            PLA: Plav_2856
            FIL: BN1229_v1_3116
            FIY: BN1229_v1_2804
            ELQ: Ga0102493_112272
            AAY: WYH_00219
            MAGQ: MGMAQ_3234
            NTH: Nther_0677
            MMI: MMAR_0861
            MLI: MULP_00886
            SVL: Strvi_1838
            SRC: M271_16300
            SGV: B1H19_33065
            SEN: SACE_3901 SACE_5862(gsmT)
            SVI: Svir_27050
            SNA: Snas_2966
            SYW: SYNW1914(gsmt)
            SYX: SynWH7803_0361
            SYNR: KR49_11730
            SYNW: SynWH8103_02187(gbmt1)
            DSL: Dacsa_3637
            PMT: PMT_0552
            PMF: P9303_17011
            HAO: PCC7418_3510
            KST: KSMBR1_0462
            PBU: L21SP3_00981
            PBP: STSP1_02231
            VBL: L21SP4_02069
            CTS: Ctha_2188
            MMH: Mmah_0524
            MHAZ: BHR79_00800
            MEV: Metev_0932
            AGW: QT03_C0001G0105
DBLINKS     ExplorEnz - The Enzyme Database: 2.1.1.156
            IUBMB Enzyme Nomenclature: 2.1.1.156
            ExPASy - ENZYME nomenclature database: 2.1.1.156
            BRENDA, the Enzyme Database: 2.1.1.156
            CAS: 294210-82-5
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