KEGG   ENZYME: 2.1.1.157Help
Entry
EC 2.1.1.157                Enzyme                                 

Name
sarcosine/dimethylglycine N-methyltransferase;
ApDMT;
sarcosine-dimethylglycine methyltransferase;
SDMT;
sarcosine dimethylglycine N-methyltransferase;
S-adenosyl-L-methionine:N,N-dimethylglycine N-methyltransferase
Class
Transferases;
Transferring one-carbon groups;
Methyltransferases
BRITE hierarchy
Sysname
S-adenosyl-L-methionine:sarcosine(or N,N-dimethylglycine) N-methyltransferase [N,N-dimethylglycine(or betaine)-forming]
Reaction(IUBMB)
2 S-adenosyl-L-methionine + sarcosine = 2 S-adenosyl-L-homocysteine + betaine (overall reaction) [RN:R10061];
(1a) S-adenosyl-L-methionine + sarcosine = S-adenosyl-L-homocysteine + N,N-dimethylglycine [RN:R07243];
(1b) S-adenosyl-L-methionine + N,N-dimethylglycine = S-adenosyl-L-homocysteine + betaine [RN:R07244]
Reaction(KEGG)
Substrate
S-adenosyl-L-methionine [CPD:C00019];
sarcosine [CPD:C00213];
N,N-dimethylglycine [CPD:C01026]
Product
S-adenosyl-L-homocysteine [CPD:C00021];
betaine [CPD:C00719];
N,N-dimethylglycine [CPD:C01026]
Comment
Cells of the oxygen-evolving halotolerant cyanobacterium Aphanocthece halophytica synthesize betaine from glycine by a three-step methylation process. The first enzyme, EC 2.1.1.156, glycine/sarcosine N-methyltransferase, leads to the formation of either sarcosine or N,N-dimethylglycine, which is further methylated to yield betaine (N,N,N-trimethylglycine) by the action of this enzyme. Both of these enzymes can catalyse the formation of N,N-dimethylglycine from sarcosine [3]. The reactions are strongly inhibited by S-adenosyl-L-homocysteine.
History
EC 2.1.1.157 created 2005, modified 2010
Pathway
ec00260  Glycine, serine and threonine metabolism
Orthology
K18897  sarcosine/dimethylglycine N-methyltransferase
Genes
TIG: THII_1899
TMB: Thimo_1799
MPUR: MARPU_03460
HHA: Hhal_1677
HHC: M911_05555
TGR: Tgr7_1180
TKM: TK90_0180
TNI: TVNIR_0235
TTI: THITH_16325
TVR: TVD_00880
HOH: Hoch_6747
PLA: Plav_2857
MAGQ: MGMAQ_3233
MMI: MMAR_0861
SEN: SACE_3901
SNA: Snas_2966
TER: Tery_2447
CTS: Ctha_2187
MMH: Mmah_0525
 » show all
Taxonomy
Reference
1  [PMID:10896953]
  Authors
Nyyssola A, Kerovuo J, Kaukinen P, von Weymarn N, Reinikainen T.
  Title
Extreme halophiles synthesize betaine from glycine by methylation.
  Journal
J Biol Chem 275:22196-201 (2000)
DOI:10.1074/jbc.M910111199
  Sequence
Reference
2  [PMID:11319079]
  Authors
Nyyssola A, Reinikainen T, Leisola M.
  Title
Characterization of glycine sarcosine N-methyltransferase and sarcosine dimethylglycine N-methyltransferase.
  Journal
Appl Environ Microbiol 67:2044-50 (2001)
DOI:10.1128/AEM.67.5.2044-2050.2001
  Sequence
Reference
3  [PMID:12466265]
  Authors
Waditee R, Tanaka Y, Aoki K, Hibino T, Jikuya H, Takano J, Takabe T, Takabe T.
  Title
Isolation and functional characterization of N-methyltransferases that catalyze betaine synthesis from glycine in a halotolerant photosynthetic organism Aphanothece halophytica.
  Journal
J Biol Chem 278:4932-42 (2003)
DOI:10.1074/jbc.M210970200
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 2.1.1.157
IUBMB Enzyme Nomenclature: 2.1.1.157
ExPASy - ENZYME nomenclature database: 2.1.1.157
BRENDA, the Enzyme Database: 2.1.1.157

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