KEGG   ENZYME: 2.1.1.179Help
Entry
EC 2.1.1.179                Enzyme                                 

Name
16S rRNA (guanine1405-N7)-methyltransferase;
methyltransferase Sgm;
m7G1405 Mtase;
Sgm Mtase;
Sgm;
sisomicin-gentamicin methyltransferase;
sisomicin-gentamicin methylase;
GrmA;
RmtB;
RmtC;
ArmA
Class
Transferases;
Transferring one-carbon groups;
Methyltransferases
BRITE hierarchy
Sysname
S-adenosyl-L-methionine:16S rRNA (guanine1405-N7)-methyltransferase
Reaction(IUBMB)
S-adenosyl-L-methionine + guanine1405 in 16S rRNA = S-adenosyl-L-homocysteine + N7-methylguanine1405 in 16S rRNA
Substrate
S-adenosyl-L-methionine [CPD:C00019];
guanine1405 in 16S rRNA
Product
S-adenosyl-L-homocysteine [CPD:C00021];
N7-methylguanine1405 in 16S rRNA
Comment
The enzyme from the antibiotic-producing bacterium Micromonospora zionensis specifically methylates guanine1405 at N7 in 16S rRNA, thereby rendering the ribosome resistant to 4,6-disubstituted deoxystreptamine aminoglycosides, which include gentamicins and kanamycins [2].
History
EC 2.1.1.179 created 2010
Orthology
K18845  16S rRNA (guanine(1405)-N(7))-methyltransferase
Genes
KPM: KPHS_p300550
KPJ: N559_5243
KPX: PMK1_ndm00227(rmtB)
KOE: A225_NDM1p0335 A225_R1p0460 A225_R1p0745
CIE: AN232_27610(rmt) AN232_29300
PRG: RB151_017530(rmtB)
ABZ: ABZJ_p00016
ABR: ABTJ_01332
ABH: M3Q_2657
ABJ: BJAB07104_p0094
CTH: Cthe_3180
KAL: KALB_7876
AFS: AFR_30330
RCA: Rcas_3259
CAU: Caur_2845
CAG: Cagg_1115
NEQ: NEQ545
NAA: Nps_01100
 » show all
Taxonomy
Reference
1  [PMID:20194115]
  Authors
Husain N, Tkaczuk KL, Tulsidas SR, Kaminska KH, Cubrilo S, Maravic-Vlahovicek G, Bujnicki JM, Sivaraman J
  Title
Structural basis for the methylation of G1405 in 16S rRNA by aminoglycoside resistance methyltransferase Sgm from an antibiotic producer: a diversity of active sites in m7G methyltransferases.
  Journal
Nucleic Acids Res 38:4120-32 (2010)
DOI:10.1093/nar/gkq122
Reference
2  [PMID:19589804]
  Authors
Savic M, Lovric J, Tomic TI, Vasiljevic B, Conn GL
  Title
Determination of the target nucleosides for members of two families of 16S rRNA methyltransferases that confer resistance to partially overlapping groups of aminoglycoside antibiotics.
  Journal
Nucleic Acids Res 37:5420-31 (2009)
DOI:10.1093/nar/gkp575
  Sequence
Reference
3  [PMID:18930134]
  Authors
Tomic TI, Moric I, Conn GL, Vasiljevic B
  Title
Aminoglycoside resistance genes sgm and kgmB protect bacterial but not yeast small ribosomal subunits in vitro despite high conservation of the rRNA A-site.
  Journal
Res Microbiol 159:658-62 (2008)
DOI:10.1016/j.resmic.2008.09.006
Reference
4  [PMID:18586937]
  Authors
Savic M, Ilic-Tomic T, Macmaster R, Vasiljevic B, Conn GL
  Title
Critical residues for cofactor binding and catalytic activity in the aminoglycoside resistance methyltransferase Sgm.
  Journal
J Bacteriol 190:5855-61 (2008)
DOI:10.1128/JB.00076-08
Reference
5  [PMID:18343347]
  Authors
Maravic Vlahovicek G, Cubrilo S, Tkaczuk KL, Bujnicki JM
  Title
Modeling and experimental analyses reveal a two-domain structure and amino acids  important for the activity of aminoglycoside resistance methyltransferase Sgm.
  Journal
Biochim Biophys Acta 1784:582-90 (2008)
DOI:10.1016/j.bbapap.2007.09.009
Reference
6  [PMID:1447159]
  Authors
Kojic M, Topisirovic L, Vasiljevic B
  Title
Cloning and characterization of an aminoglycoside resistance determinant from Micromonospora zionensis.
  Journal
J Bacteriol 174:7868-72 (1992)
DOI:10.1128/JB.174.23.7868-7872.1992
Reference
7  [PMID:19303884]
  Authors
Schmitt E, Galimand M, Panvert M, Courvalin P, Mechulam Y
  Title
Structural bases for 16 S rRNA methylation catalyzed by ArmA and RmtB methyltransferases.
  Journal
J Mol Biol 388:570-82 (2009)
DOI:10.1016/j.jmb.2009.03.034
Reference
8  [PMID:20722735]
  Authors
Wachino J, Shibayama K, Kimura K, Yamane K, Suzuki S, Arakawa Y
  Title
RmtC introduces G1405 methylation in 16S rRNA and confers high-level aminoglycoside resistance on Gram-positive microorganisms.
  Journal
FEMS Microbiol Lett 311:56-60 (2010)
DOI:10.1111/j.1574-6968.2010.02068.x
  Sequence
Reference
9  [PMID:16626740]
  Authors
Liou GF, Yoshizawa S, Courvalin P, Galimand M
  Title
Aminoglycoside resistance by ArmA-mediated ribosomal 16S methylation in human bacterial pathogens.
  Journal
J Mol Biol 359:358-64 (2006)
DOI:10.1016/j.jmb.2006.03.038
Other DBs
ExplorEnz - The Enzyme Database: 2.1.1.179
IUBMB Enzyme Nomenclature: 2.1.1.179
ExPASy - ENZYME nomenclature database: 2.1.1.179
BRENDA, the Enzyme Database: 2.1.1.179

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