KEGG   ENZYME: 2.1.1.180Help
Entry
EC 2.1.1.180                Enzyme                                 

Name
16S rRNA (adenine1408-N1)-methyltransferase;
kanamycin-apramycin resistance methylase;
16S rRNA:m1A1408 methyltransferase;
KamB;
NpmA;
16S rRNA m1A1408 methyltransferase
Class
Transferases;
Transferring one-carbon groups;
Methyltransferases
BRITE hierarchy
Sysname
S-adenosyl-L-methionine:16S rRNA (adenine1408-N1)-methyltransferase
Reaction(IUBMB)
S-adenosyl-L-methionine + adenine1408 in 16S rRNA = S-adenosyl-L-homocysteine + N1-methyladenine1408 in 16S rRNA
Substrate
S-adenosyl-L-methionine [CPD:C00019];
adenine1408 in 16S rRNA
Product
S-adenosyl-L-homocysteine [CPD:C00021];
N1-methyladenine1408 in 16S rRNA
Comment
The enzyme provides a panaminoglycoside-resistant nature through interference with the binding of aminoglycosides toward the A site of 16S rRNA through N1-methylation at position adenine1408 [4].
History
EC 2.1.1.180 created 2010
Orthology
K18846  16S rRNA (adenine(1408)-N(1))-methyltransferase
Genes
SCL: sce2330
SCU: SCE1572_13735
CCRO: CMC5_062560
ASF: SFBM_1345
ASM: MOUSESFB_1254(npmA)
ASO: SFBmNL_01416
ASB: RATSFB_1173(npmA)
TCU: Tcur_0890
AHM: TL08_05840
ACTI: UA75_05815
ACAD: UA74_05810
CAI: Caci_9046
 » show all
Taxonomy
Reference
1  [PMID:2441068]
  Authors
Beauclerk AA, Cundliffe E
  Title
Sites of action of two ribosomal RNA methylases responsible for resistance to aminoglycosides.
  Journal
J Mol Biol 193:661-71 (1987)
DOI:10.1016/0022-2836(87)90349-4
Reference
2  [PMID:17495534]
  Authors
Koscinski L, Feder M, Bujnicki JM
  Title
Identification of a missing sequence and functionally important residues of 16S rRNA:m(1)A1408 methyltransferase KamB that causes bacterial resistance to aminoglycoside antibiotics.
  Journal
Cell Cycle 6:1268-71 (2007)
DOI:10.4161/cc.6.10.4231
Reference
3  [PMID:1840536]
  Authors
Holmes DJ, Drocourt D, Tiraby G, Cundliffe E
  Title
Cloning of an aminoglycoside-resistance-encoding gene, kamC, from Saccharopolyspora hirsuta: comparison with kamB from Streptomyces tenebrarius.
  Journal
Gene 102:19-26 (1991)
DOI:10.1016/0378-1119(91)90532-G
Reference
4  [PMID:17875999]
  Authors
Wachino J, Shibayama K, Kurokawa H, Kimura K, Yamane K, Suzuki S, Shibata N, Ike Y, Arakawa Y
  Title
Novel plasmid-mediated 16S rRNA m1A1408 methyltransferase, NpmA, found in a clinically isolated Escherichia coli strain resistant to structurally diverse aminoglycosides.
  Journal
Antimicrob Agents Chemother 51:4401-9 (2007)
DOI:10.1128/AAC.00926-07
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 2.1.1.180
IUBMB Enzyme Nomenclature: 2.1.1.180
ExPASy - ENZYME nomenclature database: 2.1.1.180
BRENDA, the Enzyme Database: 2.1.1.180

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