KEGG   ENZYME: 2.1.1.188Help
Entry
EC 2.1.1.188                Enzyme                                 

Name
23S rRNA (guanine748-N1)-methyltransferase;
Rlma(II);
Rlma2;
23S rRNA m1G748 methyltransferase;
RlmaII;
Rlma II;
tylosin-resistance methyltransferase RlmA(II);
TlrB;
rRNA large subunit methyltransferase II
Class
Transferases;
Transferring one-carbon groups;
Methyltransferases
BRITE hierarchy
Sysname
S-adenosyl-L-methionine:23S rRNA (guanine748-N1)-methyltransferase
Reaction(IUBMB)
S-adenosyl-L-methionine + guanine748 in 23S rRNA = S-adenosyl-L-homocysteine + N1-methylguanine748 in 23S rRNA [RN:R07233]
Reaction(KEGG)
Substrate
S-adenosyl-L-methionine [CPD:C00019];
guanine748 in 23S rRNA
Product
S-adenosyl-L-homocysteine [CPD:C00021];
N1-methylguanine748 in 23S rRNA
Comment
The enzyme specifically methylates guanine748 at N1 in 23S rRNA. The methyltransferase RlmAII confers resistance to the macrolide antibiotic tylosin in the drug-producing strain Streptomyces fradiae [1].
History
EC 2.1.1.188 created 1976 as EC 2.1.1.51, part transferred 2010 to EC 2.1.1.188
Orthology
K14336  23S rRNA (guanine748-N1)-methyltransferase
Reference
1  [PMID:15046978]
  Authors
Douthwaite S, Crain PF, Liu M, Poehlsgaard J
  Title
The tylosin-resistance methyltransferase RlmA(II) (TlrB) modifies the N-1 position of 23S rRNA nucleotide G748.
  Journal
J Mol Biol 337:1073-7 (2004)
DOI:10.1016/j.jmb.2004.02.030
  Sequence
Reference
2  [PMID:10972803]
  Authors
Liu M, Kirpekar F, Van Wezel GP, Douthwaite S
  Title
The tylosin resistance gene tlrB of Streptomyces fradiae encodes a methyltransferase that targets G748 in 23S rRNA.
  Journal
Mol Microbiol 37:811-20 (2000)
DOI:10.1046/j.1365-2958.2000.02046.x
  Sequence
Reference
3  [PMID:12514124]
  Authors
Lebars I, Yoshizawa S, Stenholm AR, Guittet E, Douthwaite S, Fourmy D
  Title
Structure of 23S rRNA hairpin 35 and its interaction with the tylosin-resistance  methyltransferase RlmAII.
  Journal
EMBO J 22:183-92 (2003)
DOI:10.1093/emboj/cdg022
Reference
4  [PMID:17673230]
  Authors
Lebars I, Husson C, Yoshizawa S, Douthwaite S, Fourmy D
  Title
Recognition elements in rRNA for the tylosin resistance methyltransferase RlmA(II).
  Journal
J Mol Biol 372:525-34 (2007)
DOI:10.1016/j.jmb.2007.06.068
Reference
5  [PMID:18406425]
  Authors
Douthwaite S, Jakobsen L, Yoshizawa S, Fourmy D
  Title
Interaction of the tylosin-resistance methyltransferase RlmA II at its rRNA target differs from the orthologue RlmA I.
  Journal
J Mol Biol 378:969-75 (2008)
DOI:10.1016/j.jmb.2008.03.024
Reference
6  [PMID:11967079]
  Authors
Liu M, Douthwaite S
  Title
Methylation at nucleotide G745 or G748 in 23S rRNA distinguishes Gram-negative from Gram-positive bacteria.
  Journal
Mol Microbiol 44:195-204 (2002)
DOI:10.1046/j.1365-2958.2002.02866.x
Other DBs
ExplorEnz - The Enzyme Database: 2.1.1.188
IUBMB Enzyme Nomenclature: 2.1.1.188
ExPASy - ENZYME nomenclature database: 2.1.1.188
BRENDA, the Enzyme Database: 2.1.1.188

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