KEGG   ENZYME: 2.1.1.200Help
Entry
EC 2.1.1.200                Enzyme                                 

Name
tRNA (cytidine32/uridine32-2'-O)-methyltransferase;
YfhQ;
tRNA:Cm32/Um32 methyltransferase;
TrMet(Xm32);
TrmJ
Class
Transferases;
Transferring one-carbon groups;
Methyltransferases
BRITE hierarchy
Sysname
S-adenosyl-L-methionine:tRNA (cytidine32/uridine32-2'-O)-methyltransferase
Reaction(IUBMB)
(1) S-adenosyl-L-methionine + cytidine32 in tRNA = S-adenosyl-L-homocysteine + 2'-O-methylcytidine32 in tRNA;
(2) S-adenosyl-L-methionine + uridine32 in tRNA = S-adenosyl-L-homocysteine + 2'-O-methyluridine32 in tRNA
Substrate
S-adenosyl-L-methionine [CPD:C00019];
cytidine32 in tRNA;
uridine32 in tRNA
Product
S-adenosyl-L-homocysteine [CPD:C00021];
2'-O-methylcytidine32 in tRNA;
2'-O-methyluridine32 in tRNA
Comment
In Escherichia coli YfhQ is the only methyltransferase responsible for the formation of 2'-O-methylcytidine32 in tRNA. No methylation of cytosine34 in tRNALeu(CAA). In vitro the enzyme 2-O-methylates cytidine32 of tRNASer1 and uridine32 of tRNAGln2.
History
EC 2.1.1.200 created 2011
Orthology
K15396  tRNA (cytidine32/uridine32-2'-O)-methyltransferase
Genes
ECO: b2532(trmJ)
ECJ: JW2516(yfhQ)
ECD: ECDH10B_2699(yfhQ)
EBW: BWG_2296(trmJ)
ECOK: ECMDS42_2076(yfhQ)
ECE: Z3799
ECS: ECs3398
ECF: ECH74115_3764
ETW: ECSP_3476(trmJ)
ELX: CDCO157_3165
EOJ: ECO26_3579(yfhQ)
EOI: ECO111_3256(yfhQ)
EOH: ECO103_3049(yfhQ)
ECG: E2348C_2815(trmJ)
EOK: G2583_3062(trmJ)
ESO: O3O_18895
ESM: O3M_06800
ESL: O3K_06755
ELH: ETEC_2689
ECC: c3058(yfhQ)
ECP: ECP_2537
ECI: UTI89_C2854(yfhQ)
ECV: APECO1_3993(yfhQ)
ECY: ECSE_2819
ECR: ECIAI1_2584(yfhQ)
ECQ: ECED1_2963(yfhQ)
ECK: EC55989_2817(yfhQ)
ECT: ECIAI39_2733(yfhQ)
EOC: CE10_2963(trmJ)
EUM: ECUMN_2852(yfhQ)
ECZ: ECS88_2708(yfhQ)
ESE: ECSF_2376
EBR: ECB_02424(yfhQ)
EBD: ECBD_1152
EKF: KO11_10160(trmJ)
EAB: ECABU_c28380(yfhQ)
EDJ: ECDH1ME8569_2459(yfhQ)
ENA: ECNA114_2611(yfhQ)
ELW: ECW_m2758(trmJ)
ELL: WFL_13505(trmJ)
ELC: i14_2853(yfhQ)
ELD: i02_2853(yfhQ)
EBL: ECD_02424(trmJ)
EBE: B21_02388(trmJ)
ELF: LF82_2308(trmJ)
ECOI: ECOPMV1_02718(trmJ)
ECOJ: P423_13890
ECOS: EC958_2843(yfhQ)
EFE: EFER_0640(yfhQ)
STY: STY2791
STT: t0311
STM: STM2545
SENI: CY43_13585
SPT: SPA0321
SEK: SSPA0303
SEI: SPC_1106
SEC: SCH_2539(yfhQ)
SHB: SU5_03142
SENS: Q786_12600
SED: SeD_A2920
SEG: SG2580
SEL: SPUL_0330
SET: SEN2525
SENA: AU38_12795
SENO: AU37_12805
SENV: AU39_12805
SENQ: AU40_14390
SENL: IY59_13145
SEEP: I137_01505
SENE: IA1_12725
SBG: SBG_2320
SBZ: A464_2658
SFL: SF2579
SFX: S2751
SFV: SFV_2580
SFE: SFxv_2835(trmJ)
SFN: SFy_3665
SFS: SFyv_3742
SFT: NCTC1_02835(trmJ)
SSN: SSON_2614
SBO: SBO_2556
SDY: SDY_2728
ENC: ECL_03881
ECLO: ENC_37220
EEC: EcWSU1_03352(trmJ)
ECLX: LI66_16295
ECLY: LI62_18070
ECLZ: LI64_15770
ESA: ESA_00719
CSK: ES15_0991(trmJ)
CTU: CTU_31320(trmJ)
KPN: KPN_02864(yfhQ)
KPU: KP1_4116(yfhQ)
KPP: A79E_1238
KPE: KPK_1255
KPR: KPR_1411(trmJ)
KPJ: N559_1391
KPX: PMK1_00361(trmJ)
KPNU: LI86_06725
KPNK: BN49_4072(trmJ)
KVA: Kvar_1195
KOX: KOX_27370
KOE: A225_4389
EAE: EAE_00795
EAR: CCG33962
CKO: CKO_00249
CRO: ROD_24761
CAMA: F384_13915
HDE: HDEF_1743
EBT: EBL_c10260(yfhQ)
EBF: D782_1139
PSTS: E05_26880
YPE: YPO2898
YPK: y1332
YPH: YPC_3094(trmJ)
YPA: YPA_2339
YPN: YPN_1239
YPM: YP_2556(lasT)
YPZ: YPZ3_2432
YPD: YPD4_2279
YPX: YPD8_2412
YPW: CH59_3174
YPJ: CH55_1415
YPV: BZ15_631
YPL: CH46_2204
YPS: YPTB2861
YPO: BZ17_3770
YPY: YPK_1274
YPB: YPTS_2969
YPQ: DJ40_3683(trmJ)
YPU: BZ21_2163
YPR: BZ20_3348
YPC: BZ23_2445
YPF: BZ19_2230
YEN: YE1055
YEY: Y11_42691
YEW: CH47_470(trmJ)
YET: CH48_557
YAL: AT01_3527
YFR: AW19_195(trmJ)
YIN: CH53_648
YKR: CH54_1469
YRO: CH64_1484
YRU: BD65_1050
SPE: Spro_3629
SRL: SOD_c35610(trmJ)
SPLY: Q5A_019190(trmJ_2)
SSZ: SCc_566(trmJ)
SMAF: D781_3377
SMW: SMWW4_v1c37330(trmJ)
SMAR: SM39_3230(trmJ)
SMAC: SMDB11_3020(trmJ)
SERF: L085_09950
RAA: Q7S_04940
ECA: ECA3239
PATR: EV46_16055
PATO: GZ59_32540
PCT: PC1_3033
PEC: W5S_1152
SGL: SG1771
SOD: Sant_1123(trmJ)
DDD: Dda3937_02375(trmJ)
DDQ: DDI_2999
ETA: ETA_10180
EPY: EpC_10270
EPR: EPYR_01089(yfhQ)
EAM: EAMY_2599(yfhQ)
EAY: EAM_2494
EBI: EbC_33880
EGE: EM595_2685(trmJ)
PAM: PANA_2870(yfhQ)
PLF: PANA5342_1174(yfhQ)
PAJ: PAJ_2158(yfhQ)
PVA: Pvag_2291(yfhQ)
PSTW: DSJ_07480
TPTY: NCTC11468_02811(trmJ_1)
PLU: plu3285
PAY: PAU_01360(trmJ)
PMR: PMI1862
XBO: XBJ1_3030(yfhQ)
XBV: XBW1_1774(trmJ)
XNE: XNC1_3296(yfhQ)
XNM: XNC2_3173(yfhQ)
XDO: XDD1_2789(trmJ)
XPO: XPG1_1088(trmJ)
PSI: S70_03025
PSX: DR96_3777(trmJ)
PRG: RB151_028040(trmJ)
PHEI: NCTC12003_01438(trmJ)
MMK: MU9_1695
ETR: ETAE_2818
ETD: ETAF_2556
ETE: ETEE_1037
LRI: NCTC12151_00863(trmJ)
PSHI: SAMEA2665130_0562(trmJ)
HIN: HI0380
HIT: NTHI0500
HIU: HIB_04910
HIE: R2846_0200(trmJ)
HIZ: R2866_0195(trmJ)
HIA: H733_0274
HDU: HD_1664
HAP: HAPS_0165(yfiF)
HPAZ: K756_06240
HPAS: JL26_05195
HPAK: JT17_02765
HSO: HS_0279
HSM: HSM_0152
PMU: PM0316
PMV: PMCN06_0336(lasT)
PMP: Pmu_03800
PMUL: DR93_1090
MSU: MS1728(lasT)
MHAT: B824_10730
MHAE: F382_13180
MHAM: J450_11795
MHAO: J451_13415
MHAL: N220_05370
MHAQ: WC39_08580
MHAY: VK67_08580
MVR: X781_9010
MVI: X808_7730
MVG: X874_7840
APL: APL_0843
APJ: APJL_0851
APA: APP7_0900
ASU: Asuc_0862
AAT: D11S_0881
AAN: D7S_00702
AACN: AANUM_1224
BTO: WQG_10700
BTRE: F542_11360
BTRH: F543_12840
BTRA: F544_11080
XFA: XF_2475
XFT: PD_1492
XCC: XCC2276
XCB: XC_1839
XCP: XCR_2547
XCV: XCV2581
XAX: XACM_2388
XAC: XAC2384
XCI: XCAW_02049(lasT)
XFU: XFF4834R_chr23950(trmJ)
XOO: XOO2710(LasT)
XOM: XOO2555(XOO2555)
XOP: PXO_00387
XOR: XOC_2118
XAL: XALC_1924
XPH: XppCFBP6546_01095(XppCFBP6546P_01095)
SML: Smlt3320
SMT: Smal_2746
SMZ: SMD_2892(trmJ)
PSUW: WQ53_13980
PSD: DSC_05885
LEZ: GLE_3507
LEM: LEN_1629
DKO: I596_3011
VCH: VC0746
VCS: MS6_0558
VCI: O3Y_03465
VVU: VV1_0440
VVY: VV0753
VPA: VP0594
VPB: VPBB_0566
VAG: N646_2752
VSP: VS_0605
VNI: VIBNI_A2789(trmJ)
VAN: VAA_00521
VAU: VANGNB10_cI0661(trmJ)
VSC: VSVS12_00805(trmJ)
VTA: A2494(yfhQ)
VFI: VF_0615(trmJ)
VSA: VSAL_I0715(yfhQ)
AWD: AWOD_I_0590(trmJ)
PPR: PBPRA0748(Y1332)
PAE: PA3817
PAEV: N297_3942(trmJ)
PAEI: N296_3942(trmJ)
PAEP: PA1S_05970
PAEM: U769_05785
PAEL: T223_05690
PAEG: AI22_27625
PAEC: M802_3940
PAEO: M801_3808(trmJ)
PMY: Pmen_3514
PMK: MDS_3779
PRE: PCA10_11310(trmJ)
PPSE: BN5_3271(trmJ)
PCQ: PcP3B5_49970(trmJ)
PPU: PP_0839(trmJ)
PPF: Pput_0869
PPT: PPS_0905
PPI: YSA_06766
PPX: T1E_4163(yfhQ)
PPUH: B479_04690
PPUT: L483_04245
PPUN: PP4_44360(trmJ)
PPUD: DW66_0868
PMON: X969_02770
PMOT: X970_02745
PSYR: N018_19525
PFL: PFL_4968(trmJ)
PPRC: PFLCHA0_c49520(trmJ)
PPRO: PPC_4978
PFS: PFLU_5071
PFC: PflA506_4378(trmJ)
PFW: PF1751_v1c45560(trmJ)
PFB: VO64_1953
PMAN: OU5_4642
PEN: PSEEN1006
PSTT: CH92_15460
PPUU: PputUW4_00925(trmH)
PKC: PKB_1035(trmJ)
PSES: PSCI_0570
PSEM: TO66_25340
PSEC: CCOS191_0928(trmJ)
PSOS: POS17_4947
PANR: A7J50_4839
PSET: THL1_1182
PSIL: PMA3_24265
AVN: Avin_40430(trmH)
AVL: AvCA_40430(trmH)
AVD: AvCA6_40430(trmH)
ACX: Achr_10020(trmH)
PAR: Psyc_1178
PALI: A3K91_1182
PSYA: AOT82_306
ACB: A1S_1464
ABM: ABSDF1558
ABY: ABAYE2190
ABN: AB57_1695
ABX: ABK1_1957
ABAD: ABD1_14620
ABAZ: P795_9900
ABAU: IX87_02910
ABAA: IX88_09270
ACC: BDGL_000845(trmJ)
ACI: ACIAD2090
MCT: MCR_1179
MCS: DR90_732
SON: SO_2261(trmJ)
SDN: Sden_1455
SFR: Sfri_2427
SAZ: Sama_1290
SBL: Sbal_2400
SLO: Shew_2320
SSE: Ssed_2874
SPL: Spea_1485
SHL: Shal_1569
SWD: Swoo_1773
SWP: swp_1691
SVO: SVI_1588
SPSW: Sps_03595
ILO: IL2042
CPS: CPS_1129
PHA: PSHAa2673
PAT: Patl_1235
PSM: PSM_A0423
PSEO: OM33_00205
PTN: PTRA_a3208(trmJ)
PEA: PESP_a0436(trmJ)
PSPO: PSPO_a2555(trmJ)
PART: PARC_a0531(trmJ)
PTU: PTUN_a3598(trmJ)
PNG: PNIG_a3412(trmJ)
PTD: PTET_a0468(trmJ)
PSEN: PNC201_15175(trmJ)
MAQ: Maqu_1119
MHC: MARHY2162(yfhQ)
MAD: HP15_1484
MBS: MRBBS_1669(trmJ) MRBBS_1670(trmJ)
AMAL: I607_13060
AMAE: I876_13445
AMAO: I634_13305
AMAD: I636_13435
AMAI: I635_13805
AMAG: I533_13040
AMAC: MASE_12915
AAUS: EP12_13650
ASP: AOR13_773
GNI: GNIT_1016(lasT)
GPS: C427_4119
SALH: HMF8227_02280(trmJ)
PIN: Ping_1322
FBL: Fbal_2762
MVS: MVIS_0686
MYA: MORIYA_2278(yfhQ)
CJA: CJA_1464
SDE: Sde_1411
TTU: TERTU_2650(trmJ)
SAGA: M5M_04765
MICC: AUP74_01838(trmJ)
CBU: CBU_1131
CBD: CBUD_1230
CBG: CbuG_0879
CBC: CbuK_0998
LPN: lpg1747
LPH: LPV_2015(yfhQ)
LPO: LPO_1792(yfhQ)
LPM: LP6_1724
LPF: lpl1711
LPP: lpp1711
LPC: LPC_1187
LPA: lpa_02531
LPE: lp12_1685
LLO: LLO_2307(yfhQ)
LFA: LFA_1779(yfhQ)
LHA: LHA_2203(yfhQ)
LOK: Loa_02134
LCD: clem_04355(trmJ)
LLG: 44548918_00801(trmJ)
TMC: LMI_1150(yfhQ)
MCA: MCA2610
MEJ: Q7A_1497
MEC: Q7C_314
CYQ: Q91_1262
TIG: THII_2055
NOC: Noc_1646
NHL: Nhal_1927
NWA: Nwat_1482
TEE: Tel_11085
NTT: TAO_1003
AEH: Mlg_1245
HHA: Hhal_1795
TKM: TK90_0827
TNI: TVNIR_0869(trmJ_[H])
TVR: TVD_04860
GAI: IMCC3135_25645(trmJ)
HCH: HCH_04464
HCO: LOKO_00243(trmJ_1) LOKO_01608(trmJ_2)
HBE: BEI_0422(trmJ1) BEI_1491(trmJ2)
ABO: ABO_0513
ADI: B5T_03666
APAC: S7S_03635
AXE: P40_17195
KKO: Kkor_1509
KGE: TQ33_0938
TOL: TOL_0944
AHA: AHA_1745
ASA: ASA_2614
AVR: B565_2382
AMED: B224_2788
ASR: WL1483_570(trmJ)
ACAV: VI35_12360
TAU: Tola_2021
OCE: GU3_06525
SLIM: SCL_1689
SVA: SVA_1569
SALN: SALB1_3546
TBN: TBH_C2201
RMA: Rmag_0492
VOK: COSY_0453
EBH: BSEPE_0965(trmJ)
ENM: EBS_0961(lasT)
PLG: NCTC10937_01349(trmJ)
CCX: COCOR_05657(trmJ)
AFR: AFE_0670
ACU: Atc_0824
GBA: J421_3635
CABY: Cabys_205(trmJ)
 » show all
Taxonomy
Reference
1  [PMID:16848900]
  Authors
Purta E, van Vliet F, Tkaczuk KL, Dunin-Horkawicz S, Mori H, Droogmans L, Bujnicki JM
  Title
The yfhQ gene of Escherichia coli encodes a tRNA:Cm32/Um32 methyltransferase.
  Journal
BMC Mol Biol 7:23 (2006)
DOI:10.1186/1471-2199-7-23
  Sequence
[eco:b2532]
Other DBs
ExplorEnz - The Enzyme Database: 2.1.1.200
IUBMB Enzyme Nomenclature: 2.1.1.200
ExPASy - ENZYME nomenclature database: 2.1.1.200
BRENDA, the Enzyme Database: 2.1.1.200

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