KEGG   ENZYME: 2.1.1.202Help
Entry
EC 2.1.1.202                Enzyme                                 

Name
multisite-specific tRNA:(cytosine-C5)-methyltransferase;
multisite-specific tRNA:m5C-methyltransferase;
TRM4 (gene name, gene corresponding to ORF YBL024w)
Class
Transferases;
Transferring one-carbon groups;
Methyltransferases
BRITE hierarchy
Sysname
S-adenosyl-L-methionine:tRNA (cytosine-C5)-methyltransferase
Reaction(IUBMB)
(1) S-adenosyl-L-methionine + cytosine34 in tRNA precursor = S-adenosyl-L-homocysteine + 5-methylcytosine34 in tRNA precursor [RN:R00595];
(2) S-adenosyl-L-methionine + cytosine40 in tRNA precursor = S-adenosyl-L-homocysteine + 5-methylcytosine40 in tRNA precursor;
(3) S-adenosyl-L-methionine + cytosine48 in tRNA = S-adenosyl-L-homocysteine + 5-methylcytosine48 in tRNA;
(4) S-adenosyl-L-methionine + cytosine49 in tRNA = S-adenosyl-L-homocysteine + 5-methylcytosine49 in tRNA
Reaction(KEGG)
Substrate
S-adenosyl-L-methionine [CPD:C00019];
cytosine34 in tRNA precursor;
cytosine40 in tRNA precursor;
cytosine48 in tRNA;
cytosine49 in tRNA
Product
S-adenosyl-L-homocysteine [CPD:C00021];
5-methylcytosine34 in tRNA precursor;
5-methylcytosine40 in tRNA precursor;
5-methylcytosine48 in tRNA;
5-methylcytosine49 in tRNA
Comment
The enzyme from Saccharomyces cerevisiae is responsible for complete 5-methylcytosine methylations of yeast tRNA. The incidence of modification depends on the cytosine position in tRNA. At positions 34 and 40, 5-methylcytosine is found only in two yeast tRNAs (tRNALeu(CUA) and tRNAPhe(GAA), respectively), whereas most other elongator yeast tRNAs bear either 5-methylcytosine48 or 5-methylcytosine49, but never both in the same tRNA molecule [1]. The formation of 5-methylcytosine34 and 5-methylcytosine40 is a strictly intron-dependent process, whereas the formation of 5-methylcytosine48 and 5-methylcytosine49 is an intron-independent process [2,3].
History
EC 2.1.1.202 created 1976 as EC 2.1.1.29, part transferred 2011 to EC 2.1.1.202
Orthology
K15334  multisite-specific tRNA:(cytosine-C5)-methyltransferase
Genes
RSS: 109440423
ATH: AT2G22400 AT4G40000
ALY: ARALYDRAFT_320078 ARALYDRAFT_656157
CRB: 17880038 17888772
CSAT: 104702776 104713682 104720928 104751878
EUS: EUTSA_v10000050mg EUTSA_v100000640m EUTSA_v100000641m EUTSA_v10024482mg
BRP: 103837897 103854554 103864345
BNA: 106363702 106406275 106415936 106439372 106446409 111215813
BOE: 106292165 106312686 106342432
THJ: 104807678
CPAP: 110814304
CIT: 102621904
TCC: 18611524
GRA: 105787805
DZI: 111291693
LJA: Lj0g3v0259609.1(Lj0g3v0259609.1) Lj6g3v0550640.1(Lj6g3v0550640.1) Lj6g3v0550640.2(Lj6g3v0550640.2) Lj6g3v0550640.3(Lj6g3v0550640.3)
ADU: 107481819
AIP: 107631683
FVE: 101312383
PPER: 18772788
PMUM: 103333881
MDM: 103444818
PXB: 103936336
ZJU: 107425311
CSV: 101206452
CMO: 103484954
MCHA: 111005554
RCU: 8273526
JCU: 105641640
POP: 7490197
JRE: 109014563
VVI: 100247473
SIND: 105178292
OEU: 111367543
HAN: 110868434
LSV: 111901523
DCR: 108204226
BVG: 104896018
SOE: 110791853
NNU: 104604502
DOSA: Os08t0484400-01(Os08g0484400) Os09t0471900-01(Os09g0471900)
ATS: 109733225(LOC109733225) 109751909(LOC109751909)
ZMA: 100272508(gpm647) 103641349
PDA: 103707955
EGU: 105058442
MUS: 103992235
DCT: 110094233
AOF: 109832768
ATR: 18423173
PPP: 112292744
APRO: F751_0192
SCE: YBL024W(NCL1)
ERC: Ecym_2353
KMX: KLMA_30139(NCL1)
NCS: NCAS_0B05640(NCAS0B05640)
NDI: NDAI_0B01830(NDAI0B01830) NDAI_0B02980(NDAI0B02980)
TPF: TPHA_0C02870(TPHA0C02870) TPHA_0H00150(TPHA0H00150)
TBL: TBLA_0A08740(TBLA0A08740)
TDL: TDEL_0B04390(TDEL0B04390)
KAF: KAFR_0B02220(KAFR0B02220)
CAL: CAALFM_CR04300WA(CaO19.518)
CAUR: QG37_06780
SLB: AWJ20_2842(NCL1)
NCR: NCU05301
NTE: NEUTE1DRAFT80343(NEUTE1DRAFT_80343)
MGR: MGG_13217
SSCK: SPSK_01433
MAW: MAC_01383
MAJ: MAA_06410
BFU: BCIN_06g07260(Bcncl1)
MBE: MBM_04174
ANI: AN0757.2
ANG: ANI_1_1640014(An01g12180)
ABE: ARB_00062
TVE: TRV_01308
PTE: PTT_10834
CNE: CND00130
CNB: CNBD6080
MGL: MGL_1048
 » show all
Taxonomy
Reference
1  [PMID:10445884]
  Authors
Motorin Y, Grosjean H
  Title
Multisite-specific tRNA:m5C-methyltransferase (Trm4) in yeast Saccharomyces cerevisiae: identification of the gene and substrate specificity of the enzyme.
  Journal
RNA 5:1105-18 (1999)
  Sequence
[sce:YBL024W]
Reference
2  [PMID:9207014]
  Authors
Jiang HQ, Motorin Y, Jin YX, Grosjean H
  Title
Pleiotropic effects of intron removal on base modification pattern of yeast tRNAPhe: an in vitro study.
  Journal
Nucleic Acids Res 25:2694-701 (1997)
DOI:10.1093/nar/25.14.2694
Reference
3  [PMID:3537724]
  Authors
Strobel MC, Abelson J
  Title
Effect of intron mutations on processing and function of Saccharomyces cerevisiae SUP53 tRNA in vitro and in vivo.
  Journal
Mol Cell Biol 6:2663-73 (1986)
DOI:10.1128/MCB.6.7.2663
Reference
4  [PMID:17475914]
  Authors
Walbott H, Husson C, Auxilien S, Golinelli-Pimpaneau B
  Title
Cysteine of sequence motif VI is essential for nucleophilic catalysis by yeast tRNA m5C methyltransferase.
  Journal
RNA 13:967-73 (2007)
DOI:10.1261/rna.515707
Other DBs
ExplorEnz - The Enzyme Database: 2.1.1.202
IUBMB Enzyme Nomenclature: 2.1.1.202
ExPASy - ENZYME nomenclature database: 2.1.1.202
BRENDA, the Enzyme Database: 2.1.1.202

DBGET integrated database retrieval system